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Cited by 486

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Evidence for Trans splicing in trypanosomes

Open access
Sutton, Richard E., Boothroyd, John C.·1986·Cell
DOI 10.1016/0092-8674(86)90617-3
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Abstract

The 5' ends of trypanosome mRNAs consist of an identical sequence of 35 nucleotides. This "mini-exon" sequence is derived from the 5' end of a 137 nucleotide RNA (medRNA). The remainder of each mRNA is derived from a protein-coding exon that is not linked to the mini-exon. We propose that medRNA is spliced in trans to de-novo-initiated transcripts of protein-coding genes. This trans splicing model predicts that the downstream portion of medRNA will be part of a branched structure and then be released as a free product (minRNA). We demonstrate that significant levels of minRNA exist in trypanosome RNA. Furthermore, minRNA can be released from high molecular weight RNA by a HeLa cell S100 "debranching" extract. We conclude that trans splicing is the physiological process by which mature mRNA molecules are synthesized in trypanosomes.

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Metadata

Authors
Sutton, R. E., & Boothroyd, J. C.
Venue
Cell
Publisher
Elsevier BV
Published
1986-11
Volume
47
Issue
4
Pages
527-535
ISSN
0092-8674 (ISSN-L)
DOI
10.1016/0092-8674(86)90617-3
OpenAlex
W2066140926
Cited by
486
References
0
FWCI
12.57
Type
journal article
Language
en
Open access
hybrid

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CoChat. (2026). Citation check: Evidence for Trans splicing in trypanosomes. https://app.cochat.ai/papers/10.1016/0092-8674(86)90617-3
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Sutton, R. E., & Boothroyd, J. C. (1986). Evidence for Trans splicing in trypanosomes. Cell. https://doi.org/10.1016/0092-8674(86)90617-3

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Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.