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A protein factor, ASF, controls cell-specific alternative splicing of SV40 early pre-mRNA in vitro
Open accessAbstract
SV40 early pre-mRNA is alternatively spliced by utilization of two different 5' splice sites and a shared 3' splice site to produce large T and small t mRNAs. The ratio of small t to large T mRNAs produced in human embryonic kidney 293 cells is 10- to 20-fold greater than in other mammalian cells, suggesting the existence of a 293 cell-specific factor that modulates alternative splicing. Here we show that nuclear extracts from 293 cells give rise to significantly more small t splicing than do extracts from HeLa cells. Using an in vitro complementation assay, we have characterized and extensively purified a factor from 293 extracts that brings about striking increases in small t splicing with concomitant decreases in large T splicing. The factor is heat sensitive and micrococcal nuclease resistant, suggesting that it is a protein lacking an accessible RNA component. Purification of the alternative splicing factor indicates that the activity is contained in one of several possibly related polypeptides of 30-35 kd.
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Cited by
Birney, E., Kumar, S., & Krainer, A. R. (1993) · Nucleic Acids Research · doi:10.1093/nar/21.25.5803 · Cited by 668
Protein–protein interactions and 5'-splice-site recognition in mammalian mRNA precursors
Kohtz, J. D., Jamison, S. F., Will, C. L., Zuo, P., Lührmann, R., Garcia-Blanco, M. A., & Manley, J. L. (1994) · Nature · doi:10.1038/368119a0 · Cited by 623
Self-citationSharp, P. (1994) · Cell · doi:10.1016/0092-8674(94)90130-9 · Cited by 567
Mahendroo, M. S., Mendelson, C. R., & Simpson, E. R. (1993) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)36538-x · Cited by 304
Sun, Q., Mayeda, A., Hampson, R. K., Krainer, A. R., & Rottman, F. M. (1993) · Genes & Development · doi:10.1101/gad.7.12b.2598 · Cited by 284
Metadata
- Authors
- Ge, H., & Manley, J. L.
- Venue
- Cell
- Publisher
- Elsevier BV
- Published
- 1990-07
- Volume
- 62
- Issue
- 1
- Pages
- 25-34
- ISSN
- 0092-8674 (ISSN-L)
- OpenAlex
- W2057479447
- Cited by
- 477
- References
- 0
- FWCI
- 15.67
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- CoChat. (2026). Citation check: A protein factor, ASF, controls cell-specific alternative splicing of SV40 early pre-mRNA in vitro. https://app.cochat.ai/papers/10.1016/0092-8674(90)90236-8
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- Ge, H., & Manley, J. L. (1990). A protein factor, ASF, controls cell-specific alternative splicing of SV40 early pre-mRNA in vitro. Cell. https://doi.org/10.1016/0092-8674(90)90236-8
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