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Progression of the cell cycle through mitosis leads to abortion of nascent transcripts
Open accessAbstract
Nascent transcripts of the Drosophila Ubx gene were detected by in situ hybridization. Following onset of expression, the progress of RNA polymerase (1.4 kb/min) across the gene was visualized as the successive appearance of hybridization signals from different positions within the transcription unit. Nascent transcripts disappeared at mitosis. Hybridization signals reappeared in the next cell cycle first with a 5' probe, and later, following a delay consistent with the transcription rate, with a 3' probe. Nascent transcripts were observed continuously in expressing cells of a mutant embryo in which cells are blocked in interphase. We conclude that progression through mitosis causes abortion of nascent transcripts and suggest that periodic abortion of transcription contributes to regulation of expression of large genes.
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“By monitoring the appearance of RNA by in situ hybridization, nuclear run‐on, slot blot, or RT–PCR after expression activation, RNA Pol II speed was estimated between 1.1 kb/min and 4.8 kb/min (Ucker & Yamamoto, 1984; Thummel et al, 1990; Shermoen & O'Farrell, 1991; O'Brien & Lis, 1993; Tennyson et al, 1995; Femino et al, 1998; Femino et al, 2003; Hanisch et al, 2013).”
From RNA polymerase II speed: a key player in controlling and adapting transcriptome composition (2021) · cites this paper as Shermoen & O'Farrell, 1991
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Metadata
- Authors
- Shermoen, A. W., & O'Farrell, P. H.
- Affiliations
- University of California, San Francisco (US)
- Venue
- Cell
- Publisher
- Elsevier BV
- Published
- 1991-10-18
- Volume
- 67
- Issue
- 2
- Pages
- 303-310
- ISSN
- 0092-8674 (ISSN-L)
- PMC
- PMC2755073
- OpenAlex
- W1984688123
- Cited by
- 360
- References
- 32
- Citations by year
- 2026: 1 · 2025: 3 · 2024: 5 · 2023: 11 · 2022: 13 · 2021: 10 · 2020: 15 · 2019: 14 · 2018: 9 · 2017: 8 · 2016: 12 · 2015: 9 · 2014: 14 · 2013: 17 · 2012: 8
- FWCI
- 7.01
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Cell (publishedVersion)
- PubMed (publishedVersion)
- PubMed Central (acceptedVersion)
- Funders
- McKnight Foundation
- National Institutes of Health (5p01hl043821-07)
- National Heart, Lung, and Blood Institute (P0 1 HL 43821)
- National Institute of General Medical Sciences (R01 GM37193, R01GM037193)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Research and Splicing
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Nuclear Structure and Function
- Keywords
- nascent RNA, mitosis, cell cycle, RNA polymerase, Drosophila, transcriptional regulation, gene expression regulation
- MeSH terms
- Alkaline Phosphatase, Animals, Cell Nucleus, Digoxigenin, Drosophila melanogaster, Gene Expression Regulation, Genes, Homeobox, Mitosis, Mutation, Nucleic Acid Hybridization, Transcription, Genetic, DNA Probes
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