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Cited by 262Genomics and Chromatin DynamicsDevelopmental Biology and Gene RegulationNeurobiology and Insect Physiology Research

Spatial and temporal patterns of E74 transcription during Drosophila development

Thummel, Carl S., Burtis, Kenneth C., Hogness, David S.·1990·Cell
DOI 10.1016/0092-8674(90)90218-4
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Abstract

The E74 gene occupies one of the early puff loci (74EF) central to the Ashburner model for the ecdysone-induced puffing pattern in Drosophila. In support of this model, we show that the E74A promoter is directly activated by ecdysone and is subsequently repressed by ecdysone-induced proteins. Further support derives from the correspondence observed between 74EF puff size and the accumulation of nascent transcripts on the E74A unit. These transcripts elongate at 1.1 kb/min so that this 60 kb unit acts as a timer, delaying the appearance of its mRNA by 1 hr. E74A transcription is induced in a variety of ecdysone target tissues in late third instar larvae and during each of the ecdysone pulses that mark the six stages of Drosophila development. These results support an extension of the Ashburner model in which ecdysone pulses coordinate tissue development. The temporal pattern of E74B transcription overlaps but is distinct from that of E74A.

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Crossref · 10.1016/0092-8674(90)90218-4

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27 of 27 references verified0 citation errors · 0 not checkable

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  • Pioneering biochemical studies estimated average RNA Pol II speed between 1.1 kb/min and 4.8 kb/min on a few genes.

    “By monitoring the appearance of RNA by in situ hybridization, nuclear run‐on, slot blot, or RT–PCR after expression activation, RNA Pol II speed was estimated between 1.1 kb/min and 4.8 kb/min (Ucker & Yamamoto, 1984; Thummel et al, 1990; Shermoen & O'Farrell, 1991; O'Brien & Lis, 1993; Tennyson et al, 1995; Femino et al, 1998; Femino et al, 2003; Hanisch et al, 2013).”

    From RNA polymerase II speed: a key player in controlling and adapting transcriptome composition (2021) · cites this paper as Thummel et al, 1990

  • RNA Pol II velocity has been suggested to affect the timing of mRNA accumulation and could thus play a role in the timing of some developmental regulatory processes.

    “Nevertheless, RNA Pol II velocity has also been suggested to affect the timing of mRNA accumulation and could thus play a role in the timing of some developmental regulatory processes (Thummel et al, 1990; Fukaya et al, 2017; Maslon et al, 2019).”

    From RNA polymerase II speed: a key player in controlling and adapting transcriptome composition (2021) · cites this paper as Thummel et al, 1990

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Authors
Thummel, C. S., Burtis, K. C., & Hogness, D. S.
Affiliations
Stanford Medicine (US)
ORCID
Venue
Cell
Publisher
Elsevier BV
Published
1990-04
Volume
61
Issue
1
Pages
101-111
ISSN
0092-8674 (ISSN-L)
DOI
10.1016/0092-8674(90)90218-4
OpenAlex
W2052316171
Cited by
262
References
27
Citations by year
2026: 1 · 2025: 6 · 2024: 1 · 2022: 5 · 2021: 12 · 2020: 2 · 2019: 5 · 2018: 4 · 2017: 5 · 2016: 2 · 2015: 4 · 2014: 2 · 2013: 5 · 2012: 7
FWCI
9.56
Type
journal article
Language
en
Open access
closed
Licence
www.elsevier.com/tdm/userlicense/1.0/ (tdm)
Locations
  • Cell (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neurobiology and Insect Physiology Research
Keywords
puffing, ecdysone, transcriptional regulation, nascent RNA, Drosophila development, larval development
MeSH terms
Aging, Animals, Chromosomes, Drosophila melanogaster, Ecdysone, Gene Expression Regulation, Larva, Nucleic Acid Hybridization, Organ Culture Techniques, Promoter Regions, Genetic, RNA, RNA, Messenger, Salivary Glands, Transcription, Genetic, Blotting, Northern

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