A novel arachidonic acid-selective cytosolic PLA2 contains a Ca2+-dependent translocation domain with homology to PKC and GAP
Abstract
We report the cloning and expression of a cDNA encoding a high molecular weight (85.2 kd) cytosolic phospholipase A2 (cPLA2) that has no detectable sequence homology with the secreted forms of PLA2. We show that cPLA2 selectively cleaves arachidonic acid from natural membrane vesicles and demonstrate that cPLA2 translocates to membrane vesicles in response to physiologically relevant changes in free calcium. Moreover, we demonstrate that an amino-terminal 140 amino acid fragment of cPLA2 translocates to natural membrane vesicles in a Ca(2+)-dependent fashion. Interestingly, we note that this 140 amino acid domain of cPLA2 contains a 45 amino acid region with homology to PKC, p65, GAP, and PLC. We suggest that this homology delineates a Ca(2+)-dependent phospholipid-binding motif, providing a mechanism for the second messenger Ca2+ to translocate and activate cytosolic proteins.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Variation in nanomolar concentrations of intracellular Ca2+ impairs cPLA2 translocation to the cellular membrane and its subsequent activation.
“Since variation in nanomolar concentrations of intracellular Ca2+ impairs cPLA2 translocation to the cellular membrane and its subsequent activation (37)”
From Secreted Apoe rewires melanoma cell state vulnerability to ferroptosis (2024) · cites this paper as [37]
Cited by
Sorted by citations
Phospholipase A2 structure/function, mechanism, and signaling
Burke, J. E., & Dennis, E. A. (2009) · Journal of Lipid Research · doi:10.1194/jlr.r800033-jlr200 · Cited by 933 · see citing works
Lipoxygenase and leukotriene pathways: biochemistry, biology, and roles in disease
Haeggström, J. Z., & Funk, C. D. (2011) · Chemical Reviews · doi:10.1021/cr200246d · Cited by 875 · see citing works
TNF receptor subtype signalling: Differences and cellular consequences
MacEwan, D. J. (2002) · Cellular Signalling · doi:10.1016/s0898-6568(01)00262-5 · Cited by 610 · see citing works
Luminal trypsin may regulate enterocytes through proteinase-activated receptor 2
Kong, W., McConalogue, K., Khitin, L. M., Hollenberg, M. D., Payan, D. G., Böhm, S. K., & Bunnett, N. W. (1997) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.94.16.8884 · Cited by 318 · see citing works
Riehl, T. E., Alvarado, D., Ee, X., Zuckerman, A., Foster, L., Kapoor, V., Thotala, D., Ciorba, M. A., & Stenson, W. F. (2019) · Gut · doi:10.1136/gutjnl-2018-316226 · Cited by 228 · see citing works
Arachidonic acid and free fatty acids as second messengers and the role of protein kinase C
Khan, W. A., Blobe, G. C., & Hannun, Y. A. (1995) · Cellular Signalling · doi:10.1016/0898-6568(94)00089-t · Cited by 225 · see citing works
Plant adaptogens III. Earlier and more recent aspects and concepts on their mode of action
Panossian, A., Wikman, G., & Wagner, H. (1999) · Phytomedicine · doi:10.1016/s0944-7113(99)80023-3 · Cited by 211 · see citing works
Kurrasch‐Orbaugh, D. M., Parrish, J. C., Watts, V. J., & Nichols, D. E. (2003) · Journal of Neurochemistry · doi:10.1046/j.1471-4159.2003.01921.x · Cited by 138 · see citing works
Mouchlis, V. D., Bucher, D., McCammon, J. A., & Dennis, E. A. (2015) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1424651112 · Cited by 97 · see citing works
Secreted Apoe rewires melanoma cell state vulnerability to ferroptosis
More, S., Bonnereau, J., Wouters, D., Spotbeen, X., Karras, P., Rizzollo, F., Killian, T., Venken, T., Naulaerts, S., Vervoort, E., et al. (2024) · Science Advances · doi:10.1126/sciadv.adp6164 · Cited by 25 · see citing works
- Authors
- Clark, J. D., Lin, L.-L., Kriz, R. W., Ramesha, C. S., Sultzman, L. A., Lin, A. Y., Milona, N., & Knopf, J. L.
- Venue
- Cell
- Publisher
- Elsevier BV
- Published
- 1991-06
- Volume
- 65
- Issue
- 6
- Pages
- 1043-1051
- ISSN
- 0092-8674 (ISSN-L)
- OpenAlex
- W2051958752
- Cited by
- 1,688
- References
- 0
- Citations by year
- 2026: 8 · 2025: 6 · 2024: 12 · 2023: 16 · 2022: 14 · 2021: 5 · 2020: 15 · 2019: 14 · 2018: 12 · 2017: 24 · 2016: 23 · 2015: 22 · 2014: 25 · 2013: 20 · 2012: 29
- FWCI
- 43.26
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Cell (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Kinase Regulation and GTPase Signaling
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Sphingolipid Metabolism and Signaling
- Keywords
- cytosolic phospholipase A2, arachidonic acid, translocation domain, membrane vesicles, protein activation, cDNA cloning
- MeSH terms
- Amino Acid Sequence, Arachidonic Acids, Base Sequence, Binding Sites, Calcium, Cell Compartmentation, Cell Membrane, Cloning, Molecular, Cytosol, Humans, Membrane Lipids, Molecular Sequence Data, Type C Phospholipases, Phospholipases A, Phospholipids, Protein Kinase C, Proteins, Substrate Specificity, Arachidonic Acid, GTPase-Activating Proteins, Phospholipases A2
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
