Stimulatory effect on bone formation exerted by a modified chitosan
Abstract
A novel modified chitosan carrying covalently linked imidazole groups (average molecular weight 700,000, degree of substitution 0.28, degree of acetylation 0.08) was used to stimulate bone formation in an animal model. Lesions (7 mm diameter) were surgically made in the femoral condyle of sheep and treated with the modified chitosan. Within 40 d after surgery, the neoformed tissue occluded the surgical hole and assumed a trabecular structure in the peripheral area of the lesion, while looking like a mineralization nodule in the central part in association with a fibrous component. In the control, no sign of osteoinduction or reparative process was observed and bone marrow was rich in adipocytes.
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Novel 3D cell culture platforms are being created to better mimic in vivo conditions and are sometimes called spheroid or organoid culture.
“To overcome this limitation, novel 3D cell culture platforms are being created to better mimic in vivo conditions and are sometimes called spheroid or organoid culture, as described below (37,61,84,98,117,118).”
From Modeling physiological events in 2D vs. 3D cell culture (2017) · cites this paper as [84]
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Modeling physiological events in 2D vs. 3D cell culture
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- Authors
- Muzzarelli, R. A. A., Mattioli-Belmonte, M., Tietz, C., Biagini, R., Ferioli, G., Brunelli, M. A., Fini, M., Giardino, R., Ilari, P., & Biagini, G.
- Affiliations
- Istituto Ortopedico Rizzoli (IT)
- University of Bologna (IT)
- Venue
- Biomaterials
- Publisher
- Elsevier BV
- Published
- 1994-10
- Volume
- 15
- Issue
- 13
- Pages
- 1075-1081
- ISSN
- 0142-9612 (ISSN-L)
- OpenAlex
- W2005361414
- Cited by
- 284
- References
- 22
- Citations by year
- 2026: 1 · 2025: 4 · 2024: 6 · 2023: 7 · 2022: 6 · 2021: 4 · 2020: 10 · 2019: 7 · 2018: 13 · 2017: 14 · 2016: 12 · 2015: 10 · 2014: 17 · 2013: 9 · 2012: 13
- FWCI
- 4.37
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Biomaterials (publishedVersion)
- PubMed (publishedVersion)
- Università Politecnica delle Marche (Università Politecnica delle Marche) (submittedVersion)
- Topics
- Physical Sciences › Engineering › Biomedical Engineering › Bone Tissue Engineering Materials
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bone Metabolism and Diseases
- Health Sciences › Medicine › Urology › Periodontal Regeneration and Treatments
- Keywords
- modified chitosan, osteoinduction, bone formation, bone regeneration, bone defect repair, sheep model, trabecular bone formation, mineralization, bone marrow adipocytes
- MeSH terms
- Animals, Bone Development, Bone Marrow, Bone Marrow Cells, Chitin, Femur, Imidazoles, Microscopy, Electron, Models, Biological, Molecular Weight, Sheep, Fracture Healing, Adipocytes, Chitosan
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