Progestin receptor cells in mouse cerebral cortex during early postnatal development: a comparison with preoptic area and central hypothalamus using autoradiography with [125I]progestin

Shughrue, Paul J.; Stumpf, Walter E.; Elger, Walter; Schulze, Paul-Eberhard; Sar, Madhabananda

doi:10.1016/0165-3806(91)90094-y

Cited by 17Estrogen and related hormone effectsHypothalamic control of reproductive hormonesSexual Differentiation and Disorders

Progestin receptor cells in mouse cerebral cortex during early postnatal development: a comparison with preoptic area and central hypothalamus using autoradiography with [125I]progestin

Shughrue, Paul J., Stumpf, Walter E., Elger, Walter, Schulze, Paul-Eberhard, Sar, Madhabananda·1991·Developmental Brain Research

DOI 10.1016/0165-3806(91)90094-y

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Abstract

The distribution of progestin target cells in the cerebral cortex and the effect of estrogen treatment was assessed during the critical period of brain development and compared with the preoptic/central hypothalamic regions. [125I]progestin was injected into 0, 2, 8, and 12 day postnatal mice pretreated for 3 days with oil, 5 micrograms/100 g b, wt., or 100 micrograms/100 g b. wt. of estradiol dissolved in oil. Two hours after injection of radiolabeled ligand, brains were frozen and processed for thaw-mount autoradiography. At birth, labeled cells were detected in the deep (lamina VI) and intermediate (lamina V) layers of the lateral cortical regions, increased in laminae V-VI of the lateral cortex and laminae II-VI of the cingulate/paracingulate cortex at days 2 and 8, and decreased throughout the cortex by day 12. Pretreatment of animals with estradiol had no noticeable effect on the nuclear concentration of [125I]progestin in cortical cells, while estrogen weakly enhanced labeling in preoptic/central hypothalamic regions at day 2 and markedly augmented labeling in the 8 and 12 day brain. The results demonstrated that progestin receptor cells are present in the postnatal dorsal cortex, preoptic area, and hypothalamus and that the topography of cortical progestin target cells differs in part from that of estrogen target cells reported earlier.

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Blaustein, J. D., & Turcotte, J. C. (2008) · Neuroendocrinology · doi:10.1159/000125152 · Cited by 189 · see citing works

Authors: Shughrue, P. J., Stumpf, W. E., Elger, W., Schulze, P.-E., & Sar, M.
Affiliations: University of North Carolina at Chapel Hill (US)
Venue: Developmental Brain Research
Publisher: Elsevier BV
Published: 1991-04
Volume: 59
Issue: 2
Pages: 143-155
ISSN: 0165-3806 (ISSN-L)
DOI: 10.1016/0165-3806(91)90094-y
OpenAlex: W1974097905
Cited by: 17
References: 42
Citations by year: 2019: 1 · 2016: 1 · 2014: 1
FWCI: 0.86
Type: journal article
Language: en
Open access: closed
Licence: www.elsevier.com/tdm/userlicense/1.0/ (tdm)
Locations: Developmental Brain Research (publishedVersion)
PubMed (publishedVersion)
Funders: National Institutes of Health (5r01ns017479-11, 5r01ns009914-18)
National Institute of Neurological Disorders and Stroke (NS-17479, NS-09914)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Estrogen and related hormone effects
Health Sciences › Medicine › Reproductive Medicine › Hypothalamic control of reproductive hormones
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Sexual Differentiation and Disorders
Keywords: progestin receptor, cerebral cortex, postnatal brain development, preoptic area, estradiol, estrogen, cortical layers, cingulate cortex, paracingulate cortex, receptor topography
MeSH terms: Animals, Autoradiography, Brain Mapping, Cerebral Cortex, Hypothalamus, Iodine Radioisotopes, Mice, Inbred ICR, Preoptic Area, Progestins, Receptors, Progesterone, Mice

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Progestin receptor cells in mouse cerebral cortex during early postnatal development: a comparison with preoptic area and central hypothalamus using autoradiography with [125I]progestin

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