Trypanosoma cruzi receptors for human transferrin and their role
Abstract
Trypanosoma cruzi amastigotes present receptors for human transferrin as indicated by the saturable binding of 125I-transferrin to this form of the parasite. Computerized Scatchard analysis revealed one class of receptors present at 8.1 X 10(4) receptors per amastigote with a Kd of 2.82 microM. Immunofluorescence studies indicate that more than 90% of amastigotes bind human transferrin, whereas trypomastigotes do not. Iron is required for amastigote growth in cell-free medium since deferoxamine, an iron chelator, inhibits amastigote growth. Amastigote growth is restored when deferoxamine is removed from the medium. 59Fe-transferrin, which bound to amastigotes at 4 degrees C for 1 h, was readily dissociated from the parasite surface upon treatment with acid. However, this treatment did not disrupt binding that occurred at 37 degrees C for 1 h. Amastigote growth in cell-free medium is inhibited in ferrotransferrin-depleted serum, and addition of ferrotransferrin but not apotransferrin restores parasite growth. Western blots of solubilized amastigote membranes probed with anti-human transferrin receptor antibody recognize a protein of 200 kDa. This protein is present on the amastigote cell surface; therefore, human transferrin seems to interact with a 200-kDa surface amastigote protein receptor. Iron, which is essential for amastigote growth, thus appears to be delivered to T. cruzi amastigotes by transferrin receptor-mediated endocytosis.
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T. cruzi amastigotes take up Fe-loaded Tf when grown in vitro.
“The amastigotes of the intracellular parasite Trypanosoma cruzi take up Fe-loaded Tf when grown in vitro, but the physiological significance of this process is unclear [91].”
From Strategies of intracellular pathogens for obtaining iron from the environment (2015) · cites this paper as [91]
A different mechanism, a different type of transacting factor, is responsible for Fe sensing and regulation of transferrin receptor mRNA in T. brucei.
“The T. brucei genome contains only one IRP-related gene, which suggests that a different mechanism, a different type of transacting factor, is responsible for Fe sensing and regulation of transferrin receptor mRNA in this protozoan [91, 92].”
From Strategies of intracellular pathogens for obtaining iron from the environment (2015) · cites this paper as [91]
The T. brucei genome contains only one IRP-related gene.
“The T. brucei genome contains only one IRP-related gene, which suggests that a different mechanism, a different type of transacting factor, is responsible for Fe sensing and regulation of transferrin receptor mRNA in this protozoan [91, 92].”
From Strategies of intracellular pathogens for obtaining iron from the environment (2015) · cites this paper as [91]
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- Authors
- LIMA, M., & VILLALTA, F.
- Affiliations
- Meharry Medical College (US)
- Corresponding author
- VILLALTA, F
- ORCID
- Venue
- Molecular and Biochemical Parasitology
- Publisher
- Elsevier BV
- Published
- 1990-01-15
- Volume
- 38
- Issue
- 2
- Pages
- 245-252
- ISSN
- 0166-6851 (ISSN-L)
- OpenAlex
- W2157517610
- Cited by
- 58
- References
- 25
- Citations by year
- 2026: 2 · 2025: 2 · 2024: 1 · 2023: 1 · 2022: 2 · 2020: 2 · 2018: 1 · 2017: 1 · 2016: 2 · 2015: 3 · 2014: 1 · 2013: 1 · 2012: 2
- FWCI
- 4.10
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Molecular and Biochemical Parasitology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- United States Agency for International Development
- National Institutes of Health (5r29ai025637-02)
- U.S. Public Health Service
- National Institute of Allergy and Infectious Diseases (AI-07181-04, AI-25637-02)
- Topics
- Health Sciences › Medicine › Epidemiology › Trypanosoma species research and implications
- Health Sciences › Medicine › Public Health, Environmental and Occupational Health › Research on Leishmaniasis Studies
- Health Sciences › Medicine › Hematology › Iron Metabolism and Disorders
- Keywords
- Trypanosoma cruzi, human transferrin, transferrin receptor, amastigotes, iron acquisition, deferoxamine, endocytosis, trypomastigotes, iron chelation, parasite growth
- MeSH terms
- Animals, Cross Reactions, Deferoxamine, Humans, Iron, Iron Radioisotopes, Protein Binding, Receptors, Transferrin, Transferrin, Trypanosoma cruzi
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