Changes in the modulations of kinetics and allosteric properties of muscle phosphofructokinase of young and old rats
Abstract
In vitro studies on various modulations in kinetics and allosteric properties of muscle phosphofructokinase (EC 2.7.1.11) were undertaken, using purified enzyme from young (25-weeks) and old (100-weeks) albino rats. In comparison to normal K0.5 values for fructose-6-phosphate, increase in this value in response to ATP and citrate inhibition, decrease in K0.5 due to AMP activation and extent of ATP inhibition with increase in pH, were observed to be decreased more markedly with the enzyme of old than with that of young rats. Extent of citrate inhibition, reversals of ATP and citrate effects in response to AMP activation, and synergism of citrate and ATP inhibitions were also seen to be decreased considerably with muscle PFK of old in comparison to that of young rats. Such age-related changes in muscle PFK suggest the alterations in allosteric regulation of this enzyme during aging of the animal.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
The exercise-dependent increase in skeletal muscle AMP is thought to allosterically activate glycolysis and activate AMP-activated protein kinase (AMPK).
“The exercise dependent increase in skeletal muscle AMP [10] is thought to not only allosterically activate glycolysis [11] but also activate the evolutionary conserved fuel gauge protein AMP-activated protein kinase (AMPK) [12].”
From New strategies in sport nutrition to increase exercise performance (2016) · cites this paper as [11]
Cites
PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENT
Lowry, O. H., Rosebrough, N., Farr, A. L., & Randall, R. (1951) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)52451-6 · Cited by 315,771 · see citing works
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
LAEMMLI, U. K. (1970) · Nature · doi:10.1038/227680a0 · Cited by 249,961 · see citing works
GLUCONEOGENESIS AND RELATED ASPECTS OF GLYCOLYSIS
Hers, H. G., & Hue, L. (1983) · Annual Review of Biochemistry · doi:10.1146/annurev.bi.52.070183.003153 · Cited by 534 · see citing works
Control of liver 6-phosphofructokinase by fructose 2,6-bisphosphate and other effectors.
SCHAFTINGEN, E. V., Jett, M. F., Hue, L., & Hers, H. G. (1981) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.78.6.3483 · Cited by 246 · see citing works
Uyeda, K., Furuya, E., & Luby, L. J. (1981) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)68856-3 · Cited by 195 · see citing works
Allosteric regulatory properties of muscle phosphofructokinase
Kemp, R. G., & Foe, L. G. (1983) · Molecular and Cellular Biochemistry · doi:10.1007/bf00849191 · Cited by 145 · see citing works
Properties of phospho and dephospho forms of muscle phosphofructokinase.
Foe, L. G., & Kemp, R. G. (1982) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(20)65150-x · Cited by 77 · see citing works
Furuya, E., & Uyeda, K. (1980) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)70181-1 · Cited by 63 · see citing works
Sakakibara, R., & Uyeda, K. (1983) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)32107-0 · Cited by 56 · see citing works
Factors affecting the activation of rabbit muscle phosphofructokinase by actin
Kuo, H. J., Malencik, D. A., Liou, R. S., & Anderson, S. R. (1986) · Biochemistry · doi:10.1021/bi00354a013 · Cited by 54 · see citing works
Self-association of rabbit muscle phosphofructokinase: effects of ligands
Hesterberg, L. K., & Lee, J. C. (1982) · Biochemistry · doi:10.1021/bi00531a003 · Cited by 53 · see citing works
Kanungo, M. S. (1980) · Medical Entomology and Zoology · Cited by 52 · see citing works
Significance of phosphorylation of phosphofructokinase.
Kitajima, S., Sakakibara, R., & Uyeda, K. (1983) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(17)44115-9 · Cited by 35 · see citing works
Adelman, R. C., & Roth, G. S. (1983) · CRC Press eBooks · Cited by 26 · see citing works
Effects of Age on Rat Liver Enzymes
Wilson, P. D., Watson, R., & Knook, D. L. (2009) · Gerontology · doi:10.1159/000212509 · Cited by 18 · see citing works
The effect of aging on rat liver phosphoglycerate kinase and comparison with the muscle enzyme
Hiremath, L., & Rothstein, M. (1982) · Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology · doi:10.1016/0167-4838(82)90179-0 · Cited by 17 · see citing works
PHYSIOLOGICAL SIGNIFICANCE OF PHOSPHOFRUCTOKINASE ISOZYMES
Tsai, M. Y., González, F., & Kemp, R. G. (1975) · Elsevier eBooks · doi:10.1016/b978-0-12-472702-1.50055-4 · Cited by 16 · see citing works
Effect of age on the crystalline rat liver lactate dehydrogenase
Prabhakaram, M., & SINGH, S. (1986) · Archives of Gerontology and Geriatrics · doi:10.1016/0167-4943(86)90008-7 · Cited by 4 · see citing works
Self-citationAlterations in rat lactate dehydrogenase: age-related comparison of M- and H-LDH.
Prabhakaram, M., & Sn, S. (1984) · PubMed · Cited by 4 · see citing works
Differential expressions of phosphofructokinase isozymes in different tissues of young and old rats.
Sk, T., & Sn, S. (1987) · PubMed · Cited by 4 · see citing works
Metabolite-induced activation of hepatic phosphofructokinase
Proud, C. G. (1984) · Biochemical and Biophysical Research Communications · doi:10.1016/0006-291x(84)91340-8 · Cited by 3 · see citing works
Trigun, S. K., & SINGH, S. (1987) · PubMed · Cited by 1 · see citing works
Self-citation
Flagged first, then by citations
1 of the 23 references couldn’t be matched to a known paper.
Cited by
Sorted by citations
New strategies in sport nutrition to increase exercise performance
Close, G. L., Hamilton, D. L., Philp, A., Burke, L. M., & Morton, J. P. (2016) · Free Radical Biology and Medicine · doi:10.1016/j.freeradbiomed.2016.01.016 · Cited by 221 · see citing works
Mulligan, J. D., Gonzalez, A. A., Kumar, R., Davis, A. J., & Saupe, K. W. (2005) · The Journals of Gerontology Series A · doi:10.1093/gerona/60.1.21 · Cited by 50 · see citing works
Molecular Basis of Canine Muscle Type Phosphofructokinase Deficiency
Smith, B. F., Stedman, H. H., Rajpurohit, Y. R., Henthorn, P. S., Wolfe, J. H. N., Patterson, D. F., & Giger, U. (1996) · Journal of Biological Chemistry · doi:10.1074/jbc.271.33.20070 · Cited by 44 · see citing works
Gonzalez, A. A., Kumar, R., Mulligan, J. D., Davis, A. J., & Saupe, K. W. (2004) · American Journal of Physiology-Regulatory, Integrative and Comparative Physiology · doi:10.1152/ajpregu.00409.2004 · Cited by 43 · see citing works
Wang, J., Zhang, J., Qu, X., Liu, B., Song, N., Liu, Y., Wang, W., Ding, M., Wang, L., Zhang, J., et al. (2025) · Chemical Engineering Journal · doi:10.1016/j.cej.2025.161578 · Cited by 7 · see citing works
- Authors
- Trigun, S. K., & Singh, S. N.
- Affiliations
- Banaras Hindu University (IN)
- Corresponding author
- Trigun, S.K.
- ORCID
- Trigun, S.K.
- Venue
- Archives of Gerontology and Geriatrics
- Publisher
- Elsevier BV
- Published
- 1988-12
- Volume
- 7
- Issue
- 4
- Pages
- 239-247
- ISSN
- 0167-4943 (ISSN-L)
- OpenAlex
- W2087821771
- Cited by
- 5
- References
- 23
- Citations by year
- 2025: 1 · 2016: 1
- FWCI
- 0.00
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Archives of Gerontology and Geriatrics (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Biochemical and Molecular Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Metabolism, Diabetes, and Cancer
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Mitochondrial Function and Pathology
- Keywords
- enzyme kinetics, allosteric regulation, aging, age-related changes, ATP inhibition, citrate inhibition, AMP activation, fructose-6-phosphate, pH dependence
- MeSH terms
- Aging, Allosteric Regulation, Animals, Electrophoresis, Polyacrylamide Gel, Female, Glycolysis, Isoenzymes, Kinetics, Muscles, Phosphofructokinase-1, Rats, Inbred Strains, Rats, In Vitro Techniques
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
