Modifications of the 5’ untranslated region of foot-and-mouth disease virus after prolonged persistence in cell culture
Abstract
The nucleotide sequence of the 5'-untranslated region (5'UTR) of the genome of foot-and-mouth disease virus (FMDV) R100, rescued after 100 passages of persistently infected BHK-21 cells, has been compared with that of the parental FMDV C-S8c1. The nucleotide sequence divergence between the two viruses in heteropolymeric regions is 1%. The few mutations located at the 5'-most terminal region (S fragment) and at the internal ribosome entry site (IRES) do not appear to affect significantly the tight secondary structure predicted for these RNA segments. Comparison of the 5'UTR of C-S8c1 or R100 RNA with that of other FMDV serotypes and subtypes indicates the presence of block deletions (or insertions) which do not correlate with the serological classification of FMDV. Remarkably, FMDV R100, a virus highly attenuated for mice and cattle, contains a polyribocytidylate (poly C) tract of about 420 nucleotides, 145 residues longer than its parental, virulent FMDV C-S8c1. This long poly C of R100 RNA includes a few uridine residues interspersed at fairly regular intervals. This is the longest highly homopolymeric tract described in a viral genome and, to our knowledge, in any informational biomolecule.
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There is the potential for the length of the polyC tract to be artificially altered by cell culture passaging.
“However, as there is the potential for the length of the polyC tract to be artificially altered by cell culture passaging [37,40], future attempts at direct RNA sequencing should be made using fresh tissue when available.”
From Zoological transmission of encephalomyocarditis virus in the United States: Virus evolution, host ecology, and capsid antigenicity derived from an outbreak (2026) · cites this paper as [40]
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- Authors
- Escarmís, C., Toja, M., Medina, M., & Domingo, E.
- Affiliations
- Consejo Superior de Investigaciones Científicas (ES)
- Corresponding author
- Domingo, Esteban
- Venue
- Virus Research
- Publisher
- Elsevier BV
- Published
- 1992-11
- Volume
- 26
- Issue
- 2
- Pages
- 113-125
- ISSN
- 0168-1702 (ISSN-L)
- OpenAlex
- W1978205060
- Cited by
- 77
- References
- 49
- Citations by year
- 2026: 1 · 2024: 1 · 2023: 1 · 2021: 1 · 2019: 3 · 2015: 3 · 2014: 2 · 2013: 3 · 2012: 1
- FWCI
- 14.13
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Virus Research (publishedVersion)
- PubMed (publishedVersion)
- DIGITAL.CSIC (Spanish National Research Council (CSIC)) (submittedVersion)
- Funders
- Ministerio de Economía y Competitividad
- Topics
- Life Sciences › Agricultural and Biological Sciences › Agronomy and Crop Science › Animal Disease Management and Epidemiology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA and protein synthesis mechanisms
- Life Sciences › Agricultural and Biological Sciences › Ecology, Evolution, Behavior and Systematics › Vector-Borne Animal Diseases
- Keywords
- foot-and-mouth disease virus, 5' untranslated region, viral persistence, internal ribosome entry site, RNA secondary structure, poly(C) tract, attenuated virus, BHK-21 cells
- MeSH terms
- Animals, Base Sequence, Cell Line, DNA, Viral, Aphthovirus, Cricetinae, Molecular Sequence Data, Nucleic Acid Conformation, Poly C, RNA, Viral, Time Factors, Protein Biosynthesis, Virus Cultivation, Virus Replication
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