Development and advantages of serum-free, chemically defined nutrient media for culturing of nerve tissue
Abstract
This paper describes in a historical perspective the development of serum-free nutrient media suitable for long-term culturing of nerve tissue. Several disadvantages of the use of serum are discussed, coupled with an acknowledgement that it is not always advisable to replace a routinely used serum-supplemented medium by a chemically defined medium with the expectation of immediate success. Therefore a strategy is given on how to develop a chemically defined medium that is thoroughly tuned to the specific needs of the cell type to be cultured. It is argued that such a medium has several substantial advantages over the use of serum.
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Cell culture supplements provide essential nutrients and growth factors for serum-free cell culture that support cell survival, proliferation, and differentiation.
“Cell culture supplements provide essential nutrients and growth factors for serum-free cell culture that support cell survival, proliferation, and differentiation (Romijn et al., 1984; Romijn, 1988).”
From Challenges and solutions in transitioning to animal-free standards: a comprehensive analysis of components in human cell-based developmental neurotoxicity assays (2026) · cites this paper as Romijn, 1988
Cites
Observations of the living developing nerve fiber
Harrison, R. G., Greenman, M. J., Mall, F. P., & Jackson, C. M. (1907) · The Anatomical Record · doi:10.1002/ar.1090010503 · Cited by 515 · see citing works
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Spänle, J. V., Haiber, L. M., & Seeger, B. (2026) · Frontiers in Toxicology · doi:10.3389/ftox.2026.1800157 · Cited by 1 · see citing works
- Authors
- Romijn, H.
- Venue
- Biology of the Cell
- Publisher
- Wiley
- Published
- 1988
- Volume
- 63
- Issue
- 3
- Pages
- 263-268
- ISSN
- 0248-4900
- Cited by
- 44
- References
- 1
- Language
- en
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