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Isolation and characterization of DNA from the plasma of cancer patients
Abstract
Unlabelled Ten out of 37 patients with advanced malignant diseases were found to have extractable amounts of DNA in their plasma whereas no DNA could be detected in 50 normal controls. After its purification from the original nucleoprotein complex, DNA plasma levels ranging from 0.15 to 12 micrograms/ml were measured, the lowest concentration detectable with our method being 0.1 microgram/ml. Knowing from recovery experiments performed with 32P-DNA that the loss of DNA during the extraction procedure is about 65%, the real concentration of DNA in the plasma corresponds to about 3 times the given figures. The purified DNA was shown to be double-stranded and composed of fractions ranging from 21 kb to less than 0.5 kb, as determined by agarose gel electrophoresis. All these fractions hybridized with a 32P-labelled human DNA probe indicating the human origin of the bulk of the circulating DNA.In conclusion the finding of extractable amounts of DNA in the plasma of 27% of the investigated cancer patients, and its absence from the controls, suggests some correlation with malignancy.
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“The discovery of cell free DNA (cfDNA) in blood plasma has provided a simple source of genetic material for pre-natal and tumour diagnosis (13–18) that could potentially enable routine minimally invasive sampling for subsequent CNV analysis.”
From Comparison of microfluidic digital PCR and conventional quantitative PCR for measuring copy number variation (2012) · cites this paper as [14]
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Metadata
- Authors
- Stroun, M., Anker, P., Lyautey, J., Lederrey, C., & Maurice, P. A.
- Affiliations
- University of Geneva (CH)
- University Hospital of Geneva (CH)
- Venue
- European Journal of Cancer and Clinical Oncology
- Publisher
- Elsevier BV
- Published
- 1987-06
- Volume
- 23
- Issue
- 6
- Pages
- 707-712
- ISSN
- 0277-5379 (ISSN-L)
- OpenAlex
- W2058440954
- Cited by
- 373
- References
- 17
- Citations by year
- 2026: 8 · 2025: 9 · 2024: 7 · 2023: 18 · 2022: 16 · 2021: 21 · 2020: 21 · 2019: 29 · 2018: 27 · 2017: 13 · 2016: 13 · 2015: 10 · 2014: 15 · 2013: 11 · 2012: 18
- FWCI
- 0.00
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- European Journal of Cancer and Clinical Oncology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Cancer Genomics and Diagnostics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Advanced biosensing and bioanalysis techniques
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Extracellular vesicles in disease
- Keywords
- circulating tumor DNA, plasma DNA, cancer patients, DNA extraction, DNA purification, double-stranded DNA, DNA fragment size distribution, DNA hybridization, malignancy marker
- MeSH terms
- DNA, Neoplasm, Electrophoresis, Agar Gel, Humans, Neoplasms, Nucleic Acid Hybridization
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