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Contrasting effects of HEPES vs HCO3−-buffered media on whole-cell currents in cultured chemoreceptors of the rat carotid body
Abstract
In this study we compared the effects of physiological bicarbonate/CO2-buffered media (BBM) with the commonly used N-2-hydroxyethylpiperazine-N'-2-ethane-sulfonic acid (HEPES)-buffered media (HBM) on whole-cell currents in cultured rat arterial chemoreceptors (i.e. glomus cells) using the perforated-patch technique. Two separate effects were observed on switching from HBM to BBM. First, in the majority of cells tested (31 of 36) there was an increase in the leakage conductance (ca. 5 fold) and a concomitant increase in channel noise, which in preliminary studies appears to arise from the opening of large-conductance anion channels. Second, there was a reversible decrease in voltage-activated outward K+ current which we attribute to cytoplasmic acidification, catalysed by carbonic anhydrase in glomus cells.
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Existing evidence, although limited, indicates that NVBs could induce toxicity and anomalous cellular responses in particular cell types.
“However, it is vital to consider how NVBs introduce active molecules and acid-base reactions absent from mammalian fluids and existing evidence, although limited, indicates that NVBs could induce toxicity and anomalous cellular responses in particular cell types (Good et al., 1966; Lepe-Zuniga et al., 1987; Hanrahan and Tabcharani, 1990; Stea and Nurse, 1991).”
From Toward Best Practices for Controlling Mammalian Cell Culture Environments (2022) · cites this paper as Stea and Nurse, 1991
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References 17 · Cited by 27
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Metadata
- Authors
- Stea, A., & Nurse, C. A.
- Affiliations
- McMaster University (CA)
- Corresponding author
- Nurse, C.A.
- ORCID
- Nurse, C.A.
- Venue
- Neuroscience Letters
- Publisher
- Elsevier BV
- Published
- 1991-11
- Volume
- 132
- Issue
- 2
- Pages
- 239-242
- ISSN
- 0304-3940 (ISSN-L)
- OpenAlex
- W2006124657
- Cited by
- 27
- References
- 17
- Citations by year
- 2023: 1 · 2022: 1 · 2021: 2 · 2019: 1 · 2015: 1
- FWCI
- 1.20
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Neuroscience Letters (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health (1r01hl043412-01)
- National Heart, Lung, and Blood Institute (1 R01 HL 43412)
- Topics
- Life Sciences › Neuroscience › Endocrine and Autonomic Systems › Neuroscience of respiration and sleep
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Ion channel regulation and function
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Keywords
- HEPES buffer, carotid body, glomus cells, arterial chemoreceptors, whole-cell currents, leak conductance, channel noise, cytoplasmic acidification, carbonic anhydrase
- MeSH terms
- Animals, Bicarbonates, Buffers, Carotid Body, Cells, Cultured, Chemoreceptor Cells, Culture Media, Electric Conductivity, Electrophysiology, HEPES, Ions, Potassium, Rats
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