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Cited by 1,338

The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.

Muscarinic activation of ionic currents measured by a new whole-cell recording method.

Horn, R, Marty, A·1988·The Journal of general physiology
DOI 10.1085/jgp.92.2.145PMC PMC2228899
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Abstract

A new method is described as an alternative to whole-cell recording in order to prevent "wash-out" of the muscarinic response to acetylcholine (ACh) in rat lacrimal gland cells. The membrane of a cell-attached patch is permeabilized by nystatin in the patch pipette, thus providing electrical continuity between the pipette and the cytoplasm of the cell without the loss or alteration of cytoplasmic compounds necessary for the maintenance of the response to ACh. With normal whole-cell recording in these cells, the response to ACh, seen as the activation of Ca-activated K and Cl currents, lasts for approximately 5 min. With the nystatin method, the response is not diminished after 1 h. Nystatin, applied extracellularly, is shown to cause a rapid and reversible increase of membrane conductance to cations. In the absence of wash-out, we were able to obtain dose-response curves for the effect of ACh on Ca-activated K currents. An increase of [ACh] caused an increase in the K current, with apparent saturation at concentrations above approximately 1 microM ACh. The delay between ACh application and the activation of K current was inversely related to [ACh] and reached a minimum value of 0.7-1.0 s at high [ACh].

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  • Pyramidal neurons expressing eGFP were subjected to whole-cell clamping in the perforated-patch mode to prevent washout of intracellular proteins.

    “Pyramidal neurons expressing eGFP were subjected to whole-cell clamping in the perforated-patch mode to prevent washout of intracellular proteins13.”

    From Structural basis of long-term potentiation in single dendritic spines (2004) · cites this paper as [13]

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Metadata

Authors
Horn, R., & Marty, A.
Affiliations
Laboratoire de Neurobiologie, Ecole Normale Supèrieure, Paris, France.
Venue
The Journal of general physiology
Publisher
Rockefeller University Press
Published
1988-08-01
Volume
92
Issue
2
Pages
145-159
ISSN
  • 0022-1295
  • 1540-7748
DOI
10.1085/jgp.92.2.145
PMC
PMC2228899
Cited by
1,338
References
1
Language
en

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