Establishment of human microglial cell lines after transfection of primary cultures of embryonic microglial cells with the SV40 large T antigen
Abstract
Four continuous cell lines of human microglial cells were obtained by transfection of enriched cultures of human embryonic brain-derived macrophages with a plasmid encoding for the large T antigen of SV40. The transformed cells had the macrophagic characteristics of adherence and intra-cytoplasmic non-specific esterase activity. They could phagocytize zymosan particles but the phagocytic activity remained low. They expressed several macrophagic antigens but not the monocytic markers CD14, CD4, CD68/Ki-M6 and CD11c. The cells could be activated to express class II major histocompatibility complex antigens after interferon-gamma activation. Finally, interleukin-6 was produced spontaneously by the cells and this production was further increased after interleukin-1 alpha stimulation.
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HMC3 cells were originally immortalized by transfecting human embryonic microglial cells with a plasmid encoding the cDNA of the viral SV40 large T antigen.
“HMC3 cells were originally immortalized by transfecting human embryonic microglial cells with a plasmid encoding the cDNA of the viral SV40 large T antigen78.”
HMC3 cells do not secrete LOAD-relevant tumor necrosis factor α (TNF-α) or IL1-β.
“they do not secrete LOAD-relevant tumor necrosis factor α (TNF-α) or IL1-β78”
HMC3 cells maintain much of the characteristic antigenic profile of primary human microglia, including IBA1 at rest and CD68 in activated conditions.
“While these cells maintain much of the characteristic antigenic profile of primary human microglia, including IBA1 at rest and CD68 in activated conditions”
Cites
Activation of microglial cells by β-amyloid protein and interferon-γ
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Downregulation of microglial activation by apolipoprotein E and apoE-mimetic peptides
Laskowitz, D. T., Thekdi, A. D., Thekdi, S. D., Han, S. K. D., Myers, J. K., Pizzo, S. V., & Bennett, E. R. (2001) · Experimental Neurology · doi:10.1006/exnr.2001.7541 · Cited by 206 · see citing works
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Incorporation of 5 methylcytidine alleviates innate immune response to self-amplifying RNA vaccine
Komori, M., Morey, A. L., Quiñones-Molina, A. A., Fofana, J., Romero, L., Peters, E., Matsuda, K., Gummuluru, S., Smith, J. F., Akahata, W., et al. (2023) · doi:10.1101/2023.11.01.565056 · Cited by 21 · see citing works
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- Authors
- Janabi, N., Peudenier, S., Héron, B., Ng, K. H., & Tardieu, M.
- Affiliations
- Université Paris-Sud (FR)
- Inserm (FR)
- Corresponding author
- Janabi, Nazila
- Venue
- Neuroscience Letters
- Publisher
- Elsevier BV
- Published
- 1995-08
- Volume
- 195
- Issue
- 2
- Pages
- 105-108
- ISSN
- 0304-3940 (ISSN-L)
- OpenAlex
- W2071620927
- Cited by
- 280
- References
- 1
- Citations by year
- 2026: 16 · 2025: 23 · 2024: 18 · 2023: 14 · 2022: 21 · 2021: 21 · 2020: 20 · 2019: 11 · 2018: 10 · 2017: 11 · 2016: 10 · 2015: 9 · 2014: 7 · 2013: 7 · 2012: 6
- FWCI
- 1.87
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Neuroscience Letters (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Neuroscience › Neurology › Neuroinflammation and Neurodegeneration Mechanisms
- Life Sciences › Neuroscience › Developmental Neuroscience › Neurogenesis and neuroplasticity mechanisms
- Health Sciences › Medicine › Pathology and Forensic Medicine › Multiple Sclerosis Research Studies
- Keywords
- SV40 large T antigen, macrophage transformation, phagocytosis, zymosan, macrophage antigens, MHC class II expression, interferon-gamma, interleukin-6, interleukin-1 alpha
- MeSH terms
- Antibodies, Monoclonal, Antigens, Polyomavirus Transforming, Cell Line, Cell Transformation, Neoplastic, Fetus, Humans, Macrophages, Transfection, Biomarkers, Tumor, Blotting, Southern, Antigens, CD, Interleukin-6, Microglia
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