The glutathione thiol-disulfide status in the sea urchin egg during fertilization and the first cell division cycle
Abstract
The intracellular levels of GSH, GSSG, and protein-glutathione disulfide (protein-SSG) have been measured in the eggs and developing embryos of the sea urchins Lytechinus pictus and Stronglyocentrotus purpuratus. Total cellular glutathione is maintained in a very highly reduced state during these initial stages of development. Thus for unfertilized eggs of L. pictus the results (mumol/g dry weight) were 11 +/- 1 for GSH, 0.02 +/- 0.01 for GSSG, and 0.07 +/- 0.02 for protein-SSG. No significant change in these values was observed upon fertilization of the eggs or during the first cell division cycle. The values obtained with S. purpuratus were somewhat greater, but were also found to exhibit no significant variations upon fertilization or cell division. These observations indicate that changes in the total cellular glutathione thiol-disulfide status are not involved in the control mechanisms which operate during fertilization of the first cell division cycle in the sea urchin egg.
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There is no significant change in overall GSH and GSSG concentrations during fertilization and early development.
“although there is no significant change in the overall GSH and GSSG concentrations during fertilization and early development (Fahey et al., 1976)”
From The chemical defensome: Environmental sensing and response genes in the Strongylocentrotus purpuratus genome (2006) · cites this paper as Fahey et al., 1976
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Goldstone, J. V., Hamdoun, A., Cole, B. J., Howard-Ashby, M., Nebert, D. W., Scally, M., Dean, M., Epel, D., Hahn, M. E., & Stegeman, J. J. (2006) · Developmental Biology · doi:10.1016/j.ydbio.2006.08.066 · Cited by 280 · see citing works
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- Authors
- Fahey, R. C., Mikolajczyk, S. D., Meier, G. P., Epel, D., & Carroll, E. J.
- Affiliations
- University of California San Diego (US)
- Scripps Institution of Oceanography (US)
- Venue
- Biochimica et Biophysica Acta (BBA) - General Subjects
- Publisher
- Elsevier BV
- Published
- 1976-07
- Volume
- 437
- Issue
- 2
- Pages
- 445-453
- ISSN
- 0304-4165 (ISSN-L)
- OpenAlex
- W2081812526
- Cited by
- 23
- References
- 19
- Citations by year
- 2019: 1 · 2013: 1
- FWCI
- 1.41
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Biochimica et Biophysica Acta (BBA) - General Subjects (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Science Foundation
- U.S. Public Health Service
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Sulfur Compounds in Biology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Redox biology and oxidative stress
- Health Sciences › Medicine › Public Health, Environmental and Occupational Health › Reproductive Biology and Fertility
- Keywords
- glutathione, glutathione disulfide, sea urchin eggs, fertilisation (biology), first cell division, early embryonic development, Lytechinus pictus, Strongylocentrotus purpuratus, redox state
- MeSH terms
- Animals, Cell Division, Disulfides, Female, Fertilization, Glutathione, Ovum, Oxidation-Reduction, Sea Urchins, Seasons, Species Specificity, Protein Biosynthesis
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