Chemical characterization of proteases extracted from wild thistle (Cynara cardunculus)
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The enzyme preparation is heterogeneous in that three active proteinases (cynarases or cyprosins 1, 2 and 3) could be isolated, purified and partly characterised.
“This enzyme preparation was further shown to be heterogeneous in that three active proteinases (initially referred to as cynarases or cyprosins 1, 2 and 3) could be isolated, purified and partly characterised in terms of activity [6,13,19,27].”
From Advances in the role of a plant coagulant ( <i>Cynara cardunculus</i> ) in vitro and during ripening of cheeses from several milk species (2002) · cites this paper as [6]
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Roseiro, L. B., Barbosa, M., Ames, J. M., & Wilbey, R. A. (2003) · International Journal of Dairy Technology · doi:10.1046/j.1471-0307.2003.00080.x · Cited by 200 · see citing works
Sousa, M. J., & Malcata, F. X. (2002) · Le Lait · doi:10.1051/lait:2002001 · Cited by 84 · see citing works
Hydrolysis of α-lactalbumin by cardosin A immobilized on highly activated supports
Barros, R. M., Extremina, C. I., Gonçalves, I. C., Braga, B. O., Balcão, V. M., & Malcata, F. X. (2003) · Enzyme and Microbial Technology · doi:10.1016/s0141-0229(03)00244-8 · Cited by 35 · see citing works
Liburdi, K., Emiliani Spinelli, S., Benucci, I., Lombardelli, C., & Esti, M. (2018) · Food Chemistry · doi:10.1016/j.foodchem.2017.06.093 · Cited by 34 · see citing works
- Authors
- Campos, R., Guerra, R., Aguilar, M., Ventura, O., & Camacho, L.
- Affiliations
- University of Chile (CL)
- Venue
- Food Chemistry
- Publisher
- Elsevier BV
- Published
- 1990-01
- Volume
- 35
- Issue
- 2
- Pages
- 89-97
- ISSN
- 0308-8146 (ISSN-L)
- OpenAlex
- W2021459873
- Cited by
- 45
- References
- 0
- Citations by year
- 2025: 1 · 2023: 1 · 2021: 2 · 2020: 1 · 2019: 1 · 2018: 2 · 2017: 3 · 2016: 1 · 2015: 1 · 2014: 2
- FWCI
- 0.94
- Type
- journal article
- Language
- en
- Open access
- Licence
- cc-by-nc-nd
- www.elsevier.com/tdm/userlicense/1.0/ (tdm)
- Locations
- Food Chemistry (publishedVersion)
- Universidad de Chile (submittedVersion, cc-by-nc-nd)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biotechnology › Enzyme Production and Characterization
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Cynara cardunculus studies
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Pineapple and bromelain studies
- Keywords
- proteolytic enzymes, Cynara cardunculus, milk-clotting activity, proteolytic activity, ammonium sulfate precipitation, rennet, Mucor miehei, protease purification, electrophoretic patterns, pH and temperature optimum
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