Hydrolysis of α-lactalbumin by cardosin A immobilized on highly activated supports
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Cardoon flowers are traditionally used in the Mediterranean region for cheesemaking.
“Cardoon (Cynara cardunculus) flowers are traditionally used in the Mediterranean region for cheesemaking (Barros et al. 2003).”
From Plant proteases as milk-clotting enzymes in cheesemaking: a review (2014) · cites this paper as Barros et al. 2003
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Betancor, L., López‐Gallego, F., Hidalgo, A., Alonso‐Morales, N., Mateo, G. D.-O. C., Fernández‐Lafuente, R., & Guisan, J. M. (2006) · Enzyme and Microbial Technology · doi:10.1016/j.enzmictec.2006.01.014 · Cited by 415 · see citing works
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“Cardoon (Cynara cardunculus) flowers are traditionally used in the Mediterranean region for cheesemaking (Barros et al. 2003).”
BackgroundAlpha‐Lactalbumin: Its Production Technologies and Bioactive Peptides
Kamau, S. M., Cheison, S. C., Chen, W., Liu, X., & Lu, R. (2010) · Comprehensive Reviews in Food Science and Food Safety · doi:10.1111/j.1541-4337.2009.00100.x · Cited by 169 · see citing works
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“Cardoon flowers are traditionally used in the Mediterranean region for cheese making (Barros et al., 2003; De Sa and Barbosa, 1972).”
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“In addition, plant enzyme papain (Schmidt and van Markwijk, 1993), Cardosin A (Barros et al., 2003) as well as extracts from Cynara cardunculus (Barros and Malcata, 2002) were used.”
BackgroundMethodAlonso, N., López‐Gallego, F., Betancor, L., Hidalgo, A., Mateo, C., Guisan, J. M., & Fernández‐Lafuente, R. (2005) · Journal of Molecular Catalysis B Enzymatic · doi:10.1016/j.molcatb.2005.05.007 · Cited by 68 · see citing works
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Rocha, G. F., Kise, F., Rosso, A. M., & Parisi, M. G. (2017) · Food Chemistry · doi:10.1016/j.foodchem.2017.05.112 · Cited by 51 · see citing works
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Enzymatic activities of proteases immobilized on tri(4-formyl phenoxy) cyanurate
Rao, R. S., Borkar, P. S., Khobragade, C. N., & Sagar, A. K. (2006) · Enzyme and Microbial Technology · doi:10.1016/j.enzmictec.2006.03.020 · Cited by 28 · see citing works
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“Previously, aqueous extracts of C. cardunculus flowers were found to exhibit significant proteolytic activity when whole whey or whey protein concentrateswere tested (Barros et al., 2003; Barros, Ferreira, Silva, & Malcata, 2001; Barros & Malcata, 2002, 2004; Lamas, Barros, Balcão, & Malcata, 2000).”
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“Release of peptides by hydrolysis of β-LG and α-LA was monitored by RP-HPLC, as described by Barros et al. (2003).”
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“Cardosin A immobilized into agarose (6BCL)-glutaraldehyde presented the highest activity at pH 5.2 (Barros et al., 2003).”
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- Authors
- Barros, R. M., Extremina, C. I., Gonçalves, I. C., Braga, B. O., Balcão, V. M., & Malcata, F. X.
- Affiliations
- Universidade Católica Portuguesa (PT)
- Fernando Pessoa University (PT)
- Corresponding author
- Malcata, F.Xavier
- Venue
- Enzyme and Microbial Technology
- Publisher
- Elsevier BV
- Published
- 2003-12
- Volume
- 33
- Issue
- 7
- Pages
- 908-916
- ISSN
- 0141-0229 (ISSN-L)
- OpenAlex
- W1969192013
- Cited by
- 35
- References
- 34
- Citations by year
- 2025: 1 · 2024: 1 · 2022: 2 · 2021: 1 · 2020: 1 · 2019: 1 · 2018: 1 · 2017: 2 · 2015: 3 · 2014: 2 · 2013: 2 · 2012: 1
- FWCI
- 1.15
- Type
- journal article
- Language
- en
- Open access
- green
- hdl.handle.net
- Licence
- Locations
- Enzyme and Microbial Technology (publishedVersion)
- Portuguese National Funding Agency for Science, Research and Technology (RCAAP Project by FCT) (submittedVersion, other-oa)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Enzyme Catalysis and Immobilization
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biotechnology › Enzyme Production and Characterization
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Hydrolysis and Bioactive Peptides
- Keywords
- enzyme immobilization, plant proteases, whey processing, proteolysis, thermal stability, enzyme leaching, small peptides
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