Effects of the uncoupling agents FCCP and CCCP on the saltatory movements of cytoplasmic organelles
Abstract
Two potent uncoupling agents, carbonylcyanide-4-trifluoromethoxyphenylhydrazone (FCCP) and carbonylcyanide-3-chlorophenylhydrazone (CCCP) inhibit the movement of organelles in neurites of chick sensory neurones in culture. FCCP applied for 30 minutes at 10 microM reduces the number of moving organelles by 78% and a similar treatment with CCCP causes a reduction of 47%. At 100 microM either compound abolishes all directed movements both in neurites and in cultured 3T3 cells. These effects are probably not due to the discharge of proton gradients since 2,4-dinitrophenol (DNP), at concentrations shown to uncouple mitochondria by the discharge of the permeant cationic fluorescent probe rhodamine 123, fails to inhibit cytoplasmic movements. The inhibition of cytoplasmic movements by FCCP and CCCP is likely to be a consequence of their inhibitory action on a variety of enzymes, including dynein and myosin ATPases, through a reaction with sulfhydryl groups.
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FCCP and CCCP stop mitochondrial movements in 3T3 cells and chicken neurites via non-specific interaction with sulfhydryl groups of enzymes such as dynein and myosin ATPases.
“This effect relies on a non-specific interaction with sulfhydryl groups of enzymes, such as dynein and myosin ATPases [74].”
From Mitochondrial Uncoupling: A Key Controller of Biological Processes in Physiology and Diseases (2019) · cites this paper as [74]
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Mitochondrial Uncoupling: A Key Controller of Biological Processes in Physiology and Diseases
Demine, S., Renard, P., & Arnould, T. (2019) · Cells · doi:10.3390/cells8080795 · Cited by 476 · see citing works
“This effect relies on a non-specific interaction with sulfhydryl groups of enzymes, such as dynein and myosin ATPases [74].”
Background
- Authors
- HOLLENBECK, P., BRAY, D., & ADAMS, R.
- ORCID
- Venue
- Cell Biology International Reports
- Publisher
- Wiley
- Published
- 1985-02
- Volume
- 9
- Issue
- 2
- Pages
- 193-199
- ISSN
- 0309-1651 (ISSN-L)
- OpenAlex
- W2037587285
- Cited by
- 30
- References
- 15
- Citations by year
- 2024: 1 · 2023: 1 · 2022: 1 · 2021: 1 · 2020: 1 · 2019: 2 · 2018: 2 · 2017: 1 · 2016: 1 · 2015: 1 · 2013: 1 · 2012: 1
- FWCI
- 0.53
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Cell Biology International Reports (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Microtubule and mitosis dynamics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Mitochondrial Function and Pathology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
- Keywords
- FCCP, CCCP, uncoupling agents, 3T3 fibroblasts, dynein ATPase, myosin ATPase, sulfhydryl groups, proton gradient, 2,4-dinitrophenol, rhodamine 123, mitochondrial uncoupling
- MeSH terms
- Adenosine Triphosphatases, Animals, Carbonyl Cyanide m-Chlorophenyl Hydrazone, Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone, Cells, Cultured, Chick Embryo, Ganglia, Spinal, Hydrogen-Ion Concentration, Membrane Potentials, Movement, Organoids, Uncoupling Agents
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