The DOI matches its Crossref record; 19 of the 24 references it cites were found in a registry or an index.
Introduction of plasmids into whole cells of Clostridium acetobutylicum by electroporation
Open accessAbstract
A method is presented for the introduction of plasmids into Clostridium acetobutylicum ATCC 8052 by electroporation. A plasmid shuttle vector, pMTL500E, which contains the erythromycin resistance gene and replication machinery of plasmid pAMβ1, was constructed and introduced into C. acetobutylicum by electroporation. The vector was then used to introduce a 2.2 kb ClaI/SphI chromosomal fragment from C. pasteurianum into a leucine requiring mutant of C. acetobutylicum, SBA9, where complementation of auxotrophy was observed. Plasmid DNA indistinguishable from that introduced, on the basis of agarose gel electrophoresis, was observed in transformants containing either plasmid.
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“Some of the well-studied, non-acetogenic clostridia, such as the ABE model organisms C. acetobutylicum (Mermelstein et al., 1992) and C. beijerinckii (Oultram et al., 1988), and important pathogens, such as C. difficile (Ackermann et al., 2001) and C. botulinum (Zhou and Johnson, 1993), have transformation protocols that were established as early as 1988.”
From Gas Fermentation—A Flexible Platform for Commercial Scale Production of Low-Carbon-Fuels and Chemicals from Waste and Renewable Feedstocks (2016) · cites this paper as Oultram et al., 1988
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Cites
Vieira, J., & Messing, J. W. (1982) · Gene · doi:10.1016/0378-1119(82)90015-4 · Cited by 5,984
A rapid boiling method for the preparation of bacterial plasmids
Holmes, D. S., & Quigley, M. E. (1981) · Analytical Biochemistry · doi:10.1016/0003-2697(81)90473-5 · Cited by 3,198
Stable transformation of maize after gene transfer by electroporation
Fromm, M. E., Taylor, L. P., & Walbot, V. (1986) · Nature · doi:10.1038/319791a0 · Cited by 639
Improved oligonudeotide site-directed rautagenesis using M13 vectors
Carter, P. M., Bedouelle, H., & Winter, G. P. (1985) · Nucleic Acids Research · doi:10.1093/nar/13.12.4431 · Cited by 529
Chambers, S. P., Prior, S. E., Barstow, D. A., & Minton, N. P. (1988) · Gene · doi:10.1016/0378-1119(88)90606-3 · Cited by 413
Plasmid transfer by conjugation from Escherichia coli to Gram-positive bacteria
Trieu‐Cuot, P., Carlier, C., Martin, P., & Courvalin, P. M. (1987) · FEMS Microbiology Letters · doi:10.1111/j.1574-6968.1987.tb02558.x · Cited by 290
Transformation of Lactobacillus casei by electroporation
Chassy, B. M., & Flickinger, J. L. (1987) · FEMS Microbiology Letters · doi:10.1111/j.1574-6968.1987.tb02263.x · Cited by 229
Miller, J. F., Dower, W. J., & Tompkins, L. S. (1988) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.85.3.856 · Cited by 218
A Simple and Rapid Method for Genetic Transformation of Lactic Streptococci by Electroporation
Powell, I. B., Achen, M. G., Hillier, A. J., & Davidson, B. E. (1988) · Applied and Environmental Microbiology · doi:10.1128/aem.54.3.655-660.1988 · Cited by 176
Are single‐stranded circles intermediates in plasmid DNA replication?
Riele, H. T., Michel, B., & Ehrlich, S. D. (1986) · The EMBO Journal · doi:10.1002/j.1460-2075.1986.tb04257.x · Cited by 148
Research works printed but not linked: 8
- Oultram, 1985, FEMS Microbiol. Lett., 27, 129
- Oultram, 1987, FEMS Microbiol. Lett., 42, 113
- Jones, 1985, J. Gen. Microbiol., 131, 1213
- Reysset, 1988, Anaerobes Today, 239
- Somkuti, 1987, Eur. Proc. Eur. Cong. on Biotechnol., 1, 412
- Miller, 1988, Proc. Natl. Acad. Sci. USA, 85, 856
- Dower, 1987, Bio-Rad Molecular Biology Reports, 1, 5
- Oultram, 1988, Mol. Gen. Genet.
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Köpke, M., Held, C., Hujer, S., Liesegang, H., Wiezer, A., Wollherr, A., Ehrenreich, A., Liebl, W., Gottschalk, G., & Dürre, P. (2010) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1004716107 · Cited by 660
Liew, F., Martin, M. E., Tappel, R. C., Heijstra, B. D., Mihalcea, C., & Köpke, M. (2016) · Frontiers in Microbiology · doi:10.3389/fmicb.2016.00694 · Cited by 485
Bacterial alginates: from biosynthesis to applications
Remminghorst, U., & Rehm, B. H. A. (2006) · Biotechnology Letters · doi:10.1007/s10529-006-9156-x · Cited by 389
New insights and novel developments in clostridial acetone/butanol/isopropanol fermentation
Dürre, P. (1998) · Applied Microbiology and Biotechnology · doi:10.1007/s002530051226 · Cited by 336
Mermelstein, L. D., & Papoutsakis, E. T. (1993) · Applied and Environmental Microbiology · doi:10.1128/aem.59.4.1077-1081.1993 · Cited by 291
Metadata
- Authors
- Oultram, J. D., Loughlin, M., Swinfield, T.-J., Brehm, J. K., Thompson, D. E., & Minton, N. P.
- ORCID
- Minton, N.P.
- Venue
- FEMS Microbiology Letters
- Publisher
- Oxford University Press (OUP)
- Published
- 1988-11
- Volume
- 56
- Issue
- 1
- Pages
- 83-88
- ISSN
- 0378-1097 (ISSN-L)
- 1574-6968
- OpenAlex
- W1979262511
- Cited by
- 133
- References
- 24
- Citations by year
- 2024: 1 · 2023: 2 · 2022: 3 · 2021: 1 · 2019: 2 · 2017: 3 · 2016: 8 · 2015: 5 · 2014: 7 · 2013: 3 · 2012: 4
- FWCI
- 3.55
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- FEMS Microbiology Letters (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biotechnology › Microbial Inactivation Methods
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
- Keywords
- Clostridium acetobutylicum, electroporation, plasmid transformation, shuttle vector, erythromycin resistance, leucine auxotrophy
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- CoChat. (2026). Citation check: Introduction of plasmids into whole cells of Clostridium acetobutylicum by electroporation. https://app.cochat.ai/papers/10.1111/j.1574-6968.1988.tb03154.x
- The paper
- Oultram, J. D., Loughlin, M., Swinfield, T.-J., Brehm, J. K., Thompson, D. E., & Minton, N. P. (1988). Introduction of plasmids into whole cells of Clostridium acetobutylicum by electroporation. FEMS Microbiology Letters. https://doi.org/10.1111/j.1574-6968.1988.tb03154.x
