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An automated quantitative assay for fungal growth inhibition
Open accessAbstract
A simple technique which enables the monitoring of fungal growth with the aid of a microplate reader is described. In the absorbance range of 0 to 0.6 units, a straight-line relationship exists between absorbance at 595 nm and dry weight of microplate cultures, indicating that culture absorbance is an accurate indicator of fungal biomass. The relative standard deviation of the absorbance measurements was low (typically between 2 and 6%) when spores were used for inoculum. With inoculi consisting of mycelial fragments, slightly higher standard deviations (ca. 10%) were found. The microplate reader technique is particularly suited for determination of growth inhibition curves, since it is extremely fast, reliable, and requires as little as 75 μl of total culture volume.
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Cited by
Hepcidin, a urinary antimicrobial peptide synthesized in the liver
Park, C. H., Valore, E. V., Waring, A. J., & Ganz, T. (2001) · Journal of Biological Chemistry · doi:10.1074/jbc.m008922200 · Cited by 2,168
Prost, I., Dhondt, S., Rothe, G., Vicente, J., Rodriguez, M. J., Kift, N., Carbonne, F., Griffiths, G., Esquerré-Tugayé, M.-T., Rosahl, S., et al. (2005) · Plant Physiology · doi:10.1104/pp.105.066274 · Cited by 438
Identification and Rational Design of Novel Antimicrobial Peptides for Plant Protection
Marcos, J. F., Muñoz, A., Pérez-Payá, E., Misra, S., & López-García, B. (2008) · Annual Review of Phytopathology · doi:10.1146/annurev.phyto.121307.094843 · Cited by 205
Sagaram, U. S., Pandurangi, R., Kaur, J., Smith, T. J., & Shah, D. M. (2011) · PLoS ONE · doi:10.1371/journal.pone.0018550 · Cited by 185
Metadata
- Authors
- Broekaert, W.
- Venue
- FEMS Microbiology Letters
- Publisher
- Oxford University Press (OUP)
- Published
- 1990-05
- Volume
- 69
- Issue
- 1-2
- Pages
- 55-59
- ISSN
- 0378-1097 (ISSN-L)
- OpenAlex
- W1964730417
- Cited by
- 437
- References
- 0
- FWCI
- 5.01
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Broekaert, W. (1990). An automated quantitative assay for fungal growth inhibition. FEMS Microbiology Letters. https://doi.org/10.1016/0378-1097(90)90412-j
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