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Cited by 13

The DOI matches its Crossref record; 15 of the 15 references it cites were found in a registry or an index.

Lambda phagemids and their transducing properties

Melnikov, Anatolij A., Tchernov, Alexander P., Fodor, Istvan, Bayev, Alexander A.·1984·Gene
DOI 10.1016/0378-1119(84)90084-2
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Abstract

Two recombinant lambda DNAs, lambda gt::pMB9 and lambda NM::pBR322, containing, respectively, the pMB9 and pBR322 replicon were constructed and characterized. Both constructs (phagemid DNAs) transfect Escherichia coli cells, producing mature infectious phage progenies. Alternatively, drug-resistant colonies of transductants can be selected upon infection with these phages (phagemid particles) that maintain phagemid DNA in the cell in the form of covalently closed circular plasmids. The efficiency of transduction for nonlysogenic E. coli strains with lambda gt::pMB9 phage producing lambda repressor cIts ranges from 10(-7) to 10(-2) transductant colonies per input phage, depending on the temperature and strain used, while lambda NM::pBR322 phage carrying imm21 transduces with a frequency of up to 1. This means that each lambda NM::pBR322 phagemid particle is capable of establishing itself in the cell as a nonlethal plasmid, permitting formation of a resistant bacterial colony. The maximal level of transduction with lambda gt::pMB9 was obtained when E. coli cells lysogenic for lambda were used. Thus, we believe that the efficiency of transduction is determined by the turn-on of the phage repressor in the transductant. In addition, we have found that all lambda gt::pMB9-containing transductants under certain conditions harbor precisely excised pMB9; excision of pBR322 from lambda NM::pBR322 has not been observed.

Overview

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Crossref · 10.1016/0378-1119(84)90084-2

Cites

15 of 15 references verified0 citation errors · 0 not checkable

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Citations

Cites

Research works printed but not linked: 4

  1. Melnikov, 1977, In vitro construction of the vector molecule composed of the bacteriophage /gl DNA and plasmid carrying determinant for tetracycline resistance, Dokl. Acad. Nauk USSR, 236, 237
  2. Melnikov, 1984, λ phage-plasmid hybrid DNAs and their properties, Mol. Biol. (USSR), 18, N 2
  3. Melnikov, 1981, Instability of hybrid plasmids containing DNA of Drosophila melanogaster in rec+ and rec− strains of E. coli, Genetika (in Russian), 17, 45
  4. Tchernov, 1978, Studies on the structural organization of the genome of bacteriophage T5 by means of specific endonucleases, Mol. Biol. (USSR), 12, 1329

Cited by

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Metadata

Authors
Melnikov, A. A., Tchernov, A. P., Fodor, I., & Bayev, A. A.
Venue
Gene
Publisher
Elsevier BV
Published
1984-04
Volume
28
Issue
1
Pages
29-35
ISSN
0378-1119
DOI
10.1016/0378-1119(84)90084-2
Cited by
13
References
15
Language
en
Licence
www.elsevier.com/tdm/userlicense/1.0/ (tdm)

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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).

Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.