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Analysis of Cholesterol Trafficking with Fluorescent Probes

Maxfield, Frederick R., Wüstner, Daniel·2012·Methods in Cell Biology
DOI 10.1016/b978-0-12-386487-1.00017-1PMC PMC3626500
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  • Increased cholesterol in APOE4 astrocytes is due to slower exchange to HDL.

    “When astrocytes were incubated with the cholesterol efflux acceptor HDL, the percent dehydroergosterol (DHE), a natural fluorescent sterol that mimics cellular/membrane cholesterol (Maxfield and Wustner, 2012), remained significantly increased in APOE4 astrocytes after 24h (Figure S6P), indicating that increased cholesterol is due to slower exchange to HDL.”

    From Cholesterol and matrisome pathways dysregulated in astrocytes and microglia (2022) · cites this paper as Maxfield and Wustner, 2012

  • When astrocytes were incubated with the cholesterol efflux acceptor HDL, the percent dehydroergosterol (DHE) remained significantly increased in APOE4 astrocytes after 24h.

    “When astrocytes were incubated with the cholesterol efflux acceptor HDL, the percent dehydroergosterol (DHE), a natural fluorescent sterol that mimics cellular/membrane cholesterol (Maxfield and Wustner, 2012), remained significantly increased in APOE4 astrocytes after 24h (Figure S6P), indicating that increased cholesterol is due to slower exchange to HDL.”

    From Cholesterol and matrisome pathways dysregulated in astrocytes and microglia (2022) · cites this paper as Maxfield and Wustner, 2012

  • Dehydroergosterol (DHE) is a naturally occurring sterol that closely mimics the behavior of cholesterol in cells and membranes.

    “Dehydroergosterol (DHE) is a naturally-occurring sterol that closely mimics the behavior of cholesterol in cells and membranes (Hao et al., 2002; Maxfield and Wustner, 2012).”

    From Cholesterol and matrisome pathways dysregulated in astrocytes and microglia (2022) · cites this paper as Maxfield and Wustner, 2012

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Authors
Maxfield, F. R., & Wüstner, D.
Venue
Methods in Cell Biology
Publisher
Elsevier
Published
2012
Pages
367-393
ISSN
0091-679X
DOI
10.1016/b978-0-12-386487-1.00017-1
PMC
PMC3626500
Cited by
240
References
0
Language
en

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