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General Outline and Strategies for IR and Raman Spectral Interpretation
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SD-FTIR and SD-Raman spectra revealed the presence of all four characteristic stretches of hydrocarbons (νasCH3, νasCH2, νsCH3, νsCH2) in the expected region 3000-2800 cm-1.
“As SD spectra enhance the spectral resolution, the presence of all the four characteristic stretches of hydrocarbons ( ν asCH3 , ν asCH2 , ν sCH3 , ν sCH2) was revealed by the SD-FTIR (Fig. 1B) and SD-Raman spectra (Fig. 1D) in the expected region 3000 -2800 cm -1 [23,52,54].”
From Response to nutrient variation on lipid productivity in green microalgae captured using second derivative FTIR and Raman spectroscopy (2022) · cites this paper as [54]
Biomolecular groups in Chlamydomonas sp. were identified and variation in peak intensity between NP treatments and days of growth was assessed.
“Similarly, for Chlamydomonas sp ., (Fig.3 and 4) their biomolecular groups were identified and the variation in the intensity of the peaks between NP treatments and the days of growth was assessed and is tabulated in Table 3 [9,20,23,52 -54].”
From Response to nutrient variation on lipid productivity in green microalgae captured using second derivative FTIR and Raman spectroscopy (2022) · cites this paper as [54]
The ~2924 cm−1 IR band is assigned to (CH2) asymmetric stretches from hydrocarbons.
“~ 2924 | ~ 2912 | s + | vw + | m + | vw + | m + | vw + | s + |”
From Response to nutrient variation on lipid productivity in green microalgae captured using second derivative FTIR and Raman spectroscopy (2022) · cites this paper as [54]
The intensity of Raman scattering and the corresponding derivative (SD-Raman) intensity as a function of Raman shift is proportional to the analyte concentration.
“Similarly, the intensity of Raman scattering and the corresponding derivative (SD-Raman) intensity as a function of Raman shift is proportional to the analyte concentration [43,54].”
From Response to nutrient variation on lipid productivity in green microalgae captured using second derivative FTIR and Raman spectroscopy (2022) · cites this paper as [54]
The ~3010 cm−1 IR band is assigned to (=CH) stretches from unsaturated lipids.
“~ 3010 | ~ 3014 | w + | w + | w + | vw + | w + | vw + | w + |”
From Response to nutrient variation on lipid productivity in green microalgae captured using second derivative FTIR and Raman spectroscopy (2022) · cites this paper as [54]
The ~1740 cm−1 IR band is assigned to ν(C=O) stretches of esters from lipids - Triacylglycerides (TAGs).
“~ 1740 | | m + | | m + | | m + | | s + |”
From Response to nutrient variation on lipid productivity in green microalgae captured using second derivative FTIR and Raman spectroscopy (2022) · cites this paper as [54]
The ~2850 cm−1 IR band is assigned to νs(CH2) symmetric stretches.
“~ 2846 | ~ 2820 | s + | m + | s + | w + | s + | m + | vs + |”
From Response to nutrient variation on lipid productivity in green microalgae captured using second derivative FTIR and Raman spectroscopy (2022) · cites this paper as [54]
The ~1654 cm−1 IR band is assigned to ν(C=O), ν(C-N) stretches and δ(N-H) deformations of Amide I from proteins.
“~ 1654 | ~ 1658 | s + | w + | s + | vw + | s + | vw + | m + |”
From Response to nutrient variation on lipid productivity in green microalgae captured using second derivative FTIR and Raman spectroscopy (2022) · cites this paper as [54]
The ~1440 cm−1 Raman band is assigned to δ(CH2) scissoring from saturated lipids and hydrocarbons.
“| ~ 1440 | | s + | | s + | | s + |”
From Response to nutrient variation on lipid productivity in green microalgae captured using second derivative FTIR and Raman spectroscopy (2022) · cites this paper as [54]
The ~1658 cm−1 Raman band is assigned to cis ν(C=C) from unsaturated lipids.
“~ 1654 | ~ 1658 | s + | w + | s + | vw + | s + | vw + | m + |”
From Response to nutrient variation on lipid productivity in green microalgae captured using second derivative FTIR and Raman spectroscopy (2022) · cites this paper as [54]
The ~1380 cm−1 IR band is assigned to δs(CH3) symmetric bend from lipids and proteins.
“~ 1380 | | vs - | | s - | | m - | | w - |”
From Response to nutrient variation on lipid productivity in green microalgae captured using second derivative FTIR and Raman spectroscopy (2022) · cites this paper as [54]
The ~1266 cm−1 IR band is assigned to δ(=CH) deformations from unsaturated lipids.
“~ 1266 | ~ 1264 | vw + | w + | vw + | w + | vw + | w + | vw + |”
From Response to nutrient variation on lipid productivity in green microalgae captured using second derivative FTIR and Raman spectroscopy (2022) · cites this paper as [54]
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Cited by 47
Metadata
- Authors
- Larkin, P.
- Corresponding author
- Larkin, Peter
- ORCID
- Larkin, Peter
- Venue
- Infrared and Raman Spectroscopy
- Publisher
- Elsevier
- Published
- 2011
- Pages
- 117-133
- OpenAlex
- W2490613816
- Cited by
- 47
- References
- 0
- Citations by year
- 2026: 1 · 2025: 1 · 2024: 4 · 2023: 2 · 2022: 13 · 2020: 9 · 2019: 9 · 2018: 4 · 2016: 3 · 2015: 1
- FWCI
- 0.00
- Type
- book chapter
- Language
- en
- Open access
- closed
- Locations
- Elsevier eBooks (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biophysics › Spectroscopy Techniques in Biomedical and Chemical Research
- Physical Sciences › Chemistry › Analytical Chemistry › Spectroscopy and Chemometric Analyses
- Physical Sciences › Physics and Astronomy › Atomic and Molecular Physics, and Optics › Spectroscopy and Quantum Chemical Studies
- Keywords
- vibrational spectroscopy, sample preparation, reference spectral library, characteristic frequency, chemometrics, microanalysis, normal mode analysis, impurity identification
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