The DOI matches its Crossref record; 2 of the 2 references it cites were found in a registry or an index.
Quantifying Autophagic Structures in Mammalian Cells Using Confocal Microscopy
Abstract
Autophagy relies on the sequential, hierarchical association of proteins with phagophores, and forming autophagosomes to allow completion of the process. Additionally, the trafficking of the unique transmembrane autophagy-related protein ATG9 is vital for autophagy progression. In this chapter, we discuss methods to monitor autophagosome number using confocal microscopy, by following the association of different autophagosomal markers with the phagophore and completed autophagosome. We also discuss methods to monitor the trafficking of ATG9 in mammalian cells under starvation conditions.
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“An alternative way to quantify LC3 immunofluorescence staining is to estimate the percentage of LC3 signals originating from puncta over total LC3 signals in the same cell [403].”
From Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition) 1 (2021) · cites this paper as [403]
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Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition).
Klionsky, D. J., Abdelmohsen, K., Abe, A., Abedin, M. J., Abeliovich, H., Acevedo Arozena, A., Adachi, H., Adams, C. M., Adams, P. D., Adeli, K., et al. (2016) · Autophagy · doi:10.1080/15548627.2015.1100356 · Cited by 5,894
Self-citationAn Atg9-containing compartment that functions in the early steps of autophagosome biogenesis
Mari, M., Griffith, J., Rieter, E., Krishnappa, L., Klionsky, D. J., & Reggiori, F. (2010) · Journal of Cell Biology · doi:10.1083/jcb.200912089 · Cited by 491
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Research works printed but not linked: 11
- Axe, 2008, Autophagosome formation from membrane compartments enriched in phosphatidylinositol 3-phosphate and dynamically connected to the endoplasmic reticulum, The Journal of Cell Biology, 182, 685, 10.1083/jcb.200803137 · DOI · Paper page
- Birgisdottir, 2013, The LIR motif—Crucial for selective autophagy, Journal of Cell Science, 126, 3237, 10.1242/jcs.126128 · DOI
- Dooley, 2014, WIPI2 links LC3 conjugation with PI3P, autophagosome formation, and pathogen clearance by recruiting Atg12-5-16L1, Molecular Cell, 55, 238, 10.1016/j.molcel.2014.05.021 · DOI
- Kabeya, 2004, LC3, GABARAP and GATE16 localize to autophagosomal membrane depending on form-II formation, Journal of Cell Science, 117, 2805, 10.1242/jcs.01131 · DOI · Paper page
- Knævelsrud, 2013, Membrane remodeling by the PX-BAR protein SNX18 promotes autophagosome formation, The Journal of Cell Biology, 202, 331, 10.1083/jcb.201205129 · DOI
- Lamb, 2016, TBC1D14 regulates autophagy via the TRAPP complex and ATG9 traffic, The EMBO Journal, 35, 281, 10.15252/embj.201592695 · DOI
- Longatti, 2012, TBC1D14 regulates autophagosome formation via Rab11- and ULK1-positive recycling endosomes, The Journal of Cell Biology, 197, 659, 10.1083/jcb.201111079 · DOI
- Orsi, 2012, Dynamic and transient interactions of Atg9 with autophagosomes, but not membrane integration, are required for autophagy, Molecular Biology of the Cell, 23, 1860, 10.1091/mbc.E11-09-0746 · DOI
- Polson, 2010, Mammalian Atg18 (WIPI2) localizes to omegasome-anchored phagophores and positively regulates LC3 lipidation, Autophagy, 6, 506, 10.4161/auto.6.4.11863 · DOI · Paper page
- Puri, 2013, Diverse autophagosome membrane sources coalesce in recycling endosomes, Cell, 154, 1285, 10.1016/j.cell.2013.08.044 · DOI
- Young, 2006, Starvation and ULK1-dependent cycling of mammalian Atg9 between the TGN and endosomes, Journal of Cell Science, 119, 3888, 10.1242/jcs.03172 · DOI · Paper page
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Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition) 1
Klionsky, D. J., Abdel-Aziz, A. K., Abdelfatah, S., Abdellatif, M., Abdoli, A., Abel, S., Abeliovich, H., Abildgaard, M. H., Abudu, Y. P., Acevedo-Arozena, A., et al. (2021) · Autophagy · doi:10.1080/15548627.2020.1797280 · Cited by 2,897
Self-citation
Metadata
- Authors
- Lamb, C. A., Joachim, J., & Tooze, S. A.
- Venue
- Methods in Enzymology
- Publisher
- Elsevier
- Published
- 2017
- Pages
- 21-42
- ISSN
- 0076-6879
- Cited by
- 8
- References
- 2
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