CoChat
Sign Up
Cited by 68Photosynthetic Processes and MechanismsProtein Structure and DynamicsHeat shock proteins research

The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.

Structural changes associated with the acute thermal instability of Rubisco activase.

Barta, Csengele, Dunkle, Alison M., Wachter, Rebekka M., Salvucci, Michael E.·2010·Archives of Biochemistry and Biophysics
DOI 10.1016/j.abb.2010.04.022
Open this paper in CoChat — free account

Abstract

Inhibition of photosynthesis by heat has been linked to the instability of the ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) chaperone, Rubisco activase. Examination of the recombinant enzyme showed that ADP and ATP protected against inactivation, whereas Mg(2+) promoted inactivation. Heating caused aggregation of Rubisco activase characterized by disruption of secondary structure content and formation of insoluble protein. In contrast, incubation at room temperature without nucleotide caused the active approximately 660 kDa protein to form a soluble, but inactive aggregate of > 2 x 10(6) Da. Circular dichroism (CD) spectroscopy and fluorescence established that structural perturbations in the aggregate did not reduce alpha-helical content significantly. Differences in the thermal stability between wild type and mutant Rubisco activase were observed for the recombinant proteins and when the proteins were expressed in transgenic Arabidopsis. That the sensitivity of these plants to heat differs indicates that the thermal instability of Rubisco activase is a main determinant of the temperature-sensitivity of photosynthesis.

Overview

Integrity signals

Registry

Crossref · 10.1016/j.abb.2010.04.022

Cites

1 of 1 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Verification

Claims the paper makes

We couldn’t retrieve this paper’s full text, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Citations

Cites

Flagged first, then by citations

Research works printed but not linked: 42

  1. Berry, 1980, Annu. Rev. Plant Physiol., 31, 491, 10.1146/annurev.pp.31.060180.002423 · DOI · Paper page
  2. Lobell, 2003, Science, 229, 1032, 10.1126/science.1077838 · DOI
  3. Weis, 1981, Z. Pflanzenphysiol., 101, 169, 10.1016/S0044-328X(81)80051-7 · DOI
  4. Weis, 1981, Planta, 151, 33, 10.1007/BF00384234 · DOI
  5. Sharkey, 2000, Science, 287, 435, 10.1126/science.287.5452.435 · DOI
  6. Murakami, 2000, Science, 287, 476, 10.1126/science.287.5452.476 · DOI
  7. Armond, 1980, Biochim. Biophys. Acta, 601, 433, 10.1016/0005-2736(80)90547-7 · DOI
  8. Feller, 1998, Plant Physiol., 116, 539, 10.1104/pp.116.2.539 · DOI
  9. Crafts-Brandner, 2000, Planta, 212, 67, 10.1007/s004250000364 · DOI
  10. Schrader, 2004, Plant Cell Environ., 27, 725, 10.1111/j.1365-3040.2004.01172.x · DOI · Paper page
  11. Crafts-Brandner, 2000, Proc. Natl. Acad. Sci. USA, 97, 13430, 10.1073/pnas.230451497 · DOI · Paper page
  12. Sharkey, 2001, Photosyn. Res., 67, 147, 10.1023/A:1010633823747 · DOI
  13. Salvucci, 2004, Physiol. Plant., 122, 513, 10.1111/j.1399-3054.2004.00419.x · DOI
  14. Salvucci, 2004, Plant Physiol., 134, 1460, 10.1104/pp.103.038323 · DOI · Paper page
  15. Kim, 2005, Plant Cell Physiol., 46, 522, 10.1093/pcp/pci052 · DOI
  16. Salvucci, 2006, J. Exp. Bot., 57, 3793, 10.1093/jxb/erl140 · DOI · Paper page
  17. Salvucci, 2001, Plant Physiol., 127, 1053, 10.1104/pp.010357 · DOI
  18. Salvucci, 2008, J. Exp. Bot., 59, 1923, 10.1093/jxb/erm343 · DOI
  19. Sage, 2007, Plant Cell Environ., 30, 1086, 10.1111/j.1365-3040.2007.01682.x · DOI · Paper page
  20. Zhang, 2009, Plant Cell Environ., 32, 1538, 10.1111/j.1365-3040.2009.02018.x · DOI
  21. Kim, 2006, Photosyn. Res., 87, 195, 10.1007/s11120-005-8386-4 · DOI
  22. Robinson, 1989, Arch. Biochem. Biophys., 268, 93, 10.1016/0003-9861(89)90568-7 · DOI
  23. Ishijima, 2003, Arch. Biochem. Biophys., 412, 126, 10.1016/S0003-9861(03)00038-9 · DOI
  24. Chifflet, 1988, Anal. Biochem., 168, 1, 10.1016/0003-2697(88)90002-4 · DOI
  25. Bradford, 1976, Anal. Biochem., 72, 248, 10.1016/0003-2697(76)90527-3 · DOI · Paper page
  26. Wang, 1993, Biochim. Biophys. Acta, 1202, 47, 10.1016/0167-4838(93)90061-U · DOI
  27. Greenfield, 2006, Nat. Protoc., 1, 2876, 10.1038/nprot.2006.202 · DOI
  28. Whitmore, 2008, Biopolymers, 89, 392, 10.1002/bip.20853 · DOI
  29. Johnson, 1999, Proteins: Struct. Funct. Genet., 35, 307, 10.1002/(SICI)1097-0134(19990515)35:3<307::AID-PROT4>3.0.CO;2-3 · DOI
  30. Sreerama, 2000, Anal. Biochem., 287, 252, 10.1006/abio.2000.4880 · DOI
  31. Neuwald, 1999, Genome Res., 9, 27, 10.1101/gr.9.1.27 · DOI
  32. Snider, 2008, Biochem. Soc. Trans., 36, 72, 10.1042/BST0360072 · DOI
  33. Kobza, 1987, Plant Physiol., 83, 69, 10.1104/pp.83.1.69 · DOI
  34. Klein, 1995, Photosyn. Res., 43, 213, 10.1007/BF00029934 · DOI
  35. He, 1997, Plant Physiol., 115, 1569, 10.1104/pp.115.4.1569 · DOI
  36. Eckardt, 1997, Plant Physiol., 113, 575, 10.1104/pp.113.2.575 · DOI
  37. Andersson, 2008, Plant Physiol. Biochem., 46, 275, 10.1016/j.plaphy.2008.01.001 · DOI
  38. Wang, 1991, Biochim. Biophys. Acta, 1079, 263, 10.1016/0167-4838(91)90067-A · DOI
  39. Zhang, 2002, Proc. Natl. Acad. Sci. USA, 99, 3330, 10.1073/pnas.042529999 · DOI · Paper page
  40. Zhang, 2009, Photosyn. Res., 100, 29, 10.1007/s11120-009-9420-8 · DOI
  41. Kurek, 2007, Plant Cell, 19, 3230, 10.1105/tpc.107.054171 · DOI · Paper page
  42. Kumar, 2009, Photosyn. Res., 100, 143, 10.1007/s11120-009-9438-y · DOI

Cited by

Sorted by citations

Metadata

Authors
Barta, C., Dunkle, A. M., Wachter, R. M., & Salvucci, M. E.
Affiliations
  • Agricultural Research Service (US)
  • Arizona State University (US)
Corresponding author
Salvucci, Michael E.
ORCID
Venue
Archives of Biochemistry and Biophysics
Publisher
Elsevier BV
Published
2010-07
Volume
499
Issue
1-2
Pages
17-25
ISSN
0003-9861 (ISSN-L)
DOI
10.1016/j.abb.2010.04.022
OpenAlex
W2026214597
Cited by
68
References
1
Citations by year
2026: 1 · 2025: 4 · 2024: 1 · 2023: 7 · 2022: 3 · 2021: 8 · 2020: 4 · 2019: 3 · 2018: 2 · 2017: 8 · 2016: 3 · 2015: 2 · 2014: 5 · 2013: 5 · 2012: 7
FWCI
2.21
Type
journal article
Language
en
Open access
closed
Licence
www.elsevier.com/tdm/userlicense/1.0/ (tdm)
Locations
  • Archives of Biochemistry and Biophysics (publishedVersion)
  • PubMed (publishedVersion)
Funders
United States Department of Energy, Office of Basic Energy Sciences, Photosynthetic Systems (DE-FG02-08ER20268, DE-PS02-09ER09-02, FG02-09ER16123)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Photosynthetic Processes and Mechanisms
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Structure and Dynamics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Heat shock proteins research
Keywords
Rubisco activase, thermal instability, heat-induced protein aggregation, protein denaturation, secondary structure, photosynthesis inhibition, transgenic Arabidopsis, protein mutants, protein thermal stability
MeSH terms
Adenosine Diphosphate, Adenosine Triphosphate, Circular Dichroism, Enzyme Activation, Enzyme Stability, Hot Temperature, Magnesium, Photosynthesis, Plant Proteins, Protein Denaturation, Recombinant Proteins, Ribulose-Bisphosphate Carboxylase, Thermodynamics, Arabidopsis, Protein Structure, Secondary, Arabidopsis Proteins, Plants, Genetically Modified, Mutant Proteins, Protein Multimerization

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review