The DOI matches its Crossref record; 3 of the 3 references it cites were found in a registry or an index.
Fusion protein linkers: Property, design and functionality
Open accessAbstract
As an indispensable component of recombinant fusion proteins, linkers have shown increasing importance in the construction of stable, bioactive fusion proteins. This review covers the current knowledge of fusion protein linkers and summarizes examples for their design and application. The general properties of linkers derived from naturally-occurring multi-domain proteins can be considered as the foundation in linker design. Empirical linkers designed by researchers are generally classified into 3 categories according to their structures: flexible linkers, rigid linkers, and in vivo cleavable linkers. Besides the basic role in linking the functional domains together (as in flexible and rigid linkers) or releasing free functional domain in vivo (as in in vivo cleavable linkers), linkers may offer many other advantages for the production of fusion proteins, such as improving biological activity, increasing expression yield, and achieving desirable pharmacokinetic profiles.
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Cited by
Davidsson, M., Wang, G., Aldrin-Kirk, P., Cardoso, T., Nolbrant, S., Hartnor, M., Mudannayake, J., Parmar, M., & Björklund, T. (2019) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1910061116 · Cited by 142
Generation and validation of homozygous fluorescent knock-in cells using CRISPR-Cas9 genome editing
Koch, B., Nijmeijer, B., Kueblbeck, M., Cai, Y., Walther, N., & Ellenberg, J. (2018) · Nature Protocols · doi:10.1038/nprot.2018.042 · Cited by 134
Tyrosinase-Mediated Oxidative Coupling of Tyrosine Tags on Peptides and Proteins
Marmelstein, A. M., Lobba, M. J., Mogilevsky, C. S., Maza, J. C., Brauer, D. D., & Francis, M. B. (2020) · Journal of the American Chemical Society · doi:10.1021/jacs.9b12002 · Cited by 78
Design of a Cereblon construct for crystallographic and biophysical studies of protein degraders
Kroupova, A., Spiteri, V. A., Rutter, Z. J., Furihata, H., Darren, D., Ramachandran, S., Chakraborti, S., Haubrich, K., Pethe, J., Gonzales, D., et al. (2024) · Nature Communications · doi:10.1038/s41467-024-52871-9 · Cited by 47
Formation of a structurally-stable conformation by the intrinsically disordered MYC:TRRAP complex
Feris, E. J., Hinds, J. W., & Cole, M. D. (2019) · PLOS ONE · doi:10.1371/journal.pone.0225784 · Cited by 29
Metadata
- Authors
- Chen, X., Zaro, J. L., & Shen, W.-C.
- Venue
- Advanced Drug Delivery Reviews
- Publisher
- Elsevier BV
- Published
- 2013-10
- Volume
- 65
- Issue
- 10
- Pages
- 1357-1369
- ISSN
- 0169-409X (ISSN-L)
- OpenAlex
- W2020719323
- Cited by
- 2,056
- References
- 3
- FWCI
- 1.52
- Type
- journal article
- Language
- en
- Open access
- green
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