Adrenocorticotropic hormone, blood pressure, and serum erythropoietin concentrations in the rat
Abstract
BACKGROUND: This study was designed to investigate the effects of adrenocorticotropic hormone (ACTH) on systolic blood pressure (BP) and serum erythropoietin (EPO) concentrations in two strains of rats. We hypothesized that ACTH-induced hypertension in the rat is characterized by increased EPO production. METHODS: Male Sprague-Dawley (SD) and Wistar out-bred (Wistar) rats were treated with saline or ACTH (0.2 mg/kg/d). Systolic BP was measured using the tail-cuff method. Serum EPO concentrations were assayed using an ELISA kit for human EPO, which cross-reacts with but underestimates rat EPO. Thymus weight was used as a marker of glucocorticoid activity. RESULTS: In SD rats, ACTH increased systolic BP (from 109 +/- 4 to 142 +/- 5 mm Hg, P <.0005), significantly greater than in saline-treated rats (P <.01). Systolic BP in ACTH-treated Wistar increased from 120 +/- 3 to 133 +/- 4 mm Hg (P <.05), but was not significantly different from saline-treated Wistar rats. The ACTH-induced increase in systolic BP was greater in SD than in Wistar rats (P <.05). Serum EPO levels were 5.6 +/- 0.4 in SD and 5.9 +/- 0.3 IU/L in Wistar rats, and decreased to undetectable levels with ACTH treatment in 10 of 10 SD and 7 of 10 Wistar rats. The ACTH treatment increased hemoglobin and hematocrit, and decreased thymus weight in both strains. CONCLUSIONS: 1) ACTH decreased serum EPO concentrations in both strains; 2) EPO is inversely related to ACTH-induced hypertension in the rat; and 3) Wistar rats are relatively resistant to the BP raising effects of ACTH treatment but not to the ACTH-induced decrease in thymus weight. These data suggest that EPO is not causal in ACTH-induced hypertension.
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Chronic high circulating levels of ACTH, caused by excess anterior pituitary secretion such as Cushing's disease or by infusion of ACTH, are associated with hypertension in humans and experimental animals.
“Chronic high circulating levels of ACTH, caused by excess anterior pituitary secretion (e.g. Cushing’s disease) or by infusion of ACTH, are associated with hypertension in humans and experimental animals [10–12].”
From Role of the brain melanocortins in blood pressure regulation (2017) · cites this paper as [11]
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Role of the brain melanocortins in blood pressure regulation
do Carmo, J. M., da Silva, A. A., Wang, Z., Fang, T., Aberdein, N., Perez de Lara, C. E., & Hall, J. E. (2017) · Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease · doi:10.1016/j.bbadis.2017.03.003 · Cited by 38 · see citing works
- Authors
- Zhang, Y.
- Affiliations
- Australian National University (AU)
- Corresponding author
- Zhang, Y
- ORCID
- Zhang, Y
- Venue
- American Journal of Hypertension
- Publisher
- Oxford University Press (OUP)
- Published
- 2004-05
- Volume
- 17
- Issue
- 5
- Pages
- 457-461
- ISSN
- 0895-7061 (ISSN-L)
- OpenAlex
- W1984276414
- Cited by
- 10
- References
- 31
- Citations by year
- 2020: 1 · 2017: 2 · 2013: 1
- FWCI
- 1.05
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- American Journal of Hypertension (publishedVersion)
- PubMed (publishedVersion)
- ANU Open Research (Australian National University) (submittedVersion)
- Funders
- Novartis Australia
- Topics
- Health Sciences › Medicine › Hematology › Erythropoietin and Anemia Treatment
- Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Hormonal Regulation and Hypertension
- Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Adrenal Hormones and Disorders
- Keywords
- ACTH, ACTH-induced hypertension, erythropoietin, systolic blood pressure, rat strain differences, Sprague-Dawley rats, Wistar rats, thymus weight, glucocorticoid activity, hemoglobin, hematocrit, rat model of hypertension
- MeSH terms
- Adrenocorticotropic Hormone, Animals, Blood Glucose, Blood Pressure, Body Weight, Erythropoietin, Hematocrit, Hemoglobins, Injections, Intravenous, Male, Models, Cardiovascular, Placebos, Sodium Chloride, Systole, Thymus Gland, Biomarkers, Rats, Sprague-Dawley, Rats, Wistar, Models, Animal, Rats
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