Cited by 123Protein Hydrolysis and Bioactive PeptidesAquaculture Nutrition and GrowthFood Nutrient Composition and Product Development

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Utilization of tilapia processing waste for the production of fish protein hydrolysate

Silva, J.F.X., Ribeiro, K., Silva, J.F., Cahú, T.B., Bezerra, R.S.·2014·Animal Feed Science and Technology

DOI 10.1016/j.anifeedsci.2014.06.010

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Abstract

Viscera and carcasses represent 60–70% of whole fish body weight, and this processing waste is a known source of bioactive molecules, including proteases. These enzymes can be employed in various biotechnological processes, such as, for preparing fish protein hydrolysate (FPH). Therefore, the objective of this study was to evaluate the use of processing waste from Nile tilapia (Oreochromis niloticus) as a source of protein and proteases to produce FPH. Three FPH production conditions were evaluated: two conditions used autolysis with enzymes extracted from the tilapia intestine at different concentrations (FPH100, 100mg of tissue/mL and FPH600, 600mg of tissue/mL) and the third used 0.5% (v/v) Alcalase (FPHcom), a commercial protease preparation. Protein, amino acids and fatty acids content were calculated as DM basis. After a 4-h reaction, maximum hydrolysis percentages from FPHcom, FPH100, and FPH600 systems were 34.73±1.44%, 29.21±0.79%, and 41.66±1.33%, respectively. The protein content in the resulting FPS was 584.8g/kg, 492.3g/kg, and 508.2g/kg for FPHcom, FPH100, and FPH600, respectively. Methionine and lysine were found at levels of 32.0 and 77.0g/kg (FPHcom), 31.0 and 64.0g/kg (FPH100), and 33.0 and 69.0g/kg (FPH600), respectively. Polyunsaturated fatty acid contents of FPHcom, FPH100, and FPH600 were 101.0g/kg, 138.0g/kg, and 70g/kg, respectively, with a predominance of linoleic acid (C18:2n−6). Amino acid composition, lipid profile, and amino acid score suggested that all of the experimental FPHs could be employed as a protein source in diets for aquatic organisms and other farmed animals.

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Authors: Silva, J. F. X., Ribeiro, K., Silva, J. F., Cahú, T. B., & Bezerra, R. S.
Affiliations: Universidade Federal de Pernambuco (BR)
Universidade Federal do Rio Grande do Norte (BR)
ORCID: Ribeiro, K.
Cahú, T.B.
Bezerra, R.S.
Venue: Animal Feed Science and Technology
Publisher: Elsevier BV
Published: 2014-10
Volume: 196
Pages: 96-106
ISSN: 0377-8401 (ISSN-L)
DOI: 10.1016/j.anifeedsci.2014.06.010
OpenAlex: W1997895484
Cited by: 123
References: 45
Citations by year: 2026: 5 · 2025: 11 · 2024: 14 · 2023: 9 · 2022: 6 · 2021: 20 · 2020: 9 · 2019: 14 · 2018: 9 · 2017: 12 · 2016: 9 · 2015: 5
FWCI: 3.27
Type: journal article
Language: en
Open access: closed
Licence: www.elsevier.com/tdm/userlicense/1.0/ (tdm)
Locations: Animal Feed Science and Technology (publishedVersion)
Funders: Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Ministério da Pesca e Aquicultura (MPA)
Fundação de Amparo a Ciência e Tecnologia do Estado de Pernambuco (FACEPE)
Topics: Life Sciences › Agricultural and Biological Sciences › Food Science › Protein Hydrolysis and Bioactive Peptides
Life Sciences › Agricultural and Biological Sciences › Aquatic Science › Aquaculture Nutrition and Growth
Life Sciences › Agricultural and Biological Sciences › Food Science › Food Nutrient Composition and Product Development
Keywords: fish protein hydrolysate, Oreochromis niloticus, fish waste, fish viscera, fish carcasses, proteolytic enzymes, autolysis, alcalase, proteolysis, amino acid composition, fatty acid composition, polyunsaturated fatty acids, methionine, lysine, fish feed protein source

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Utilization of tilapia processing waste for the production of fish protein hydrolysate

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