Cited by 15Protein Hydrolysis and Bioactive PeptidesFood and Agricultural SciencesAquaculture Nutrition and Growth

Chemical Characteristics and Amino Acids Profile of Protein Hydrolysates of Nile Tilapia (Oreochromis niloticus) Viscera.

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Riyadi, Putut Har, Suprayitno, Eddy, Aulanni'am, Aulanni'am, Sulistiyati, Titik Dwi·2019·Journal of World's Poultry Research

Abstract

Research on protein hydrolysate has been performed by using various types of fish and enzymes, but there is limited research on the nutritive value of visceral waste proteins of tilapia.The present study aimed to determine amino acid profile and composition (water, protein, fat and ash content) of protein hydrolysates prepared from viscera of Nile tilapia (Oreochromis niloticus).Alcalase enzyme was used as the hydrolytic enzyme at a concentration of 1.5 % (w/v), pH 7.9, and temperature of 55.80 °C for 1.5 h.Fresh Nile tilapia viscera had a high protein content of 35.14% ± 0.02 (dry basis) and the defatting process reduced fat content from 60.24 ± 0.04 to 57.81% ± 0.01 (dry basis).The results indicated that the hydrolysis of Nile tilapia viscera led to an increase in the protein content (62.81% ± 0.18) (dry basis).Furthermore, hydrolysis process also decreased the moisture content (11.56 % ± 0.49), fat content (16% ± 0.14), and ash content (5% ± 0.17) (dry basis).Glutamine had the highest amino acid level in hydrolysates (3.85 g/100g), whereas cysteine the lowest level (0.32 g/100g).In conclusion, Nile tilapia protein hydrolysates contain sufficient quantities of the essential amino acids that can be used as a source for fish feed protein.Moreover, chemical characteristics and amino acid profile of Nile tilapia protein hydrolysates indicated a high nutritional value which could be met adult human nutritional needs.

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  1. [13]13. Nurhayati T, Salamah E and Nugraha R (2014). Optimization Process Production Hydrolysates of Protein Barramudi Viscera. Jurnal Pengolah Hasil Perikanan, 17(1): 42-52. Available at: https://journal.ipb.ac.id/index.php/jphpi/article/download/8136/6385
  2. [16]16. Roslan J, Kamal M, Yunos KF and Abdullah N (2015). Optimization of enzymatic hydrolysis of tilapia (Oreochromis niloticus) by- product using response surface methodology. International Food Research Journal, 22(3): 1117-1123. Available at: http://www.ifrj.upm.edu.my/22%20(03)%202015/(34).pdf

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Authors
Riyadi, P. H., Suprayitno, E., Aulanni'am, A., & Sulistiyati, T. D.
Affiliations
  • Diponegoro University (ID)
  • University of Brawijaya (ID)
Corresponding author
Riyadi, Putut Har
Venue
Journal of World's Poultry Research
Published
2019-12-25
Volume
9
Issue
4
Pages
324-328
ISSN
2322-455X (ISSN-L)
OpenAlex
W3016475387
Cited by
15
References
19
Citations by year
2026: 4 · 2025: 3 · 2024: 3 · 2022: 1 · 2021: 3 · 2020: 1
FWCI
0.41
Type
journal article
Language
en
Open access
Locations
  • Journal of World s Poultry Research (publishedVersion)
  • CyberLeninK (CyberLeninka) (submittedVersion, cc-by)
Funders
Lembaga Pengelola Dana Pendidikan
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Hydrolysis and Bioactive Peptides
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Food and Agricultural Sciences
  • Life Sciences › Agricultural and Biological Sciences › Aquatic Science › Aquaculture Nutrition and Growth
Keywords
Oreochromis niloticus, fish viscera, protein hydrolysates, alcalase hydrolysis, amino acid composition, essential amino acids, fish feed protein, fish waste utilization, nutritional value

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