In utero exposure to gestational diabetes mellitus conditions TLR4 and TLR2 activated IL-1beta responses in spleen cells from rat offspring
Open accessAbstract
Fetal exposure to gestational diabetes mellitus (GDM) is associated with a higher risk of youth-onset insulin resistance and type 2 diabetes. We have previously shown that the rat offspring of GDM dams are insulin resistant when compared to the offspring of lean dams. Since inflammation influences insulin sensitivity, we examined the impact of fetal exposure to GDM on inflammatory responses in the offspring. In rats, we compared inflammatory activity in newborn pups as well as 16week-old young-adult offspring from lean control dams with offspring from high fat and sucrose diet (HFS)-induced GDM dams. To determine whether there are additive effects of exposure to GDM and post-weaning diets, offspring of lean and GDM dams were fed either low fat or HFS diets until 16weeks of age. Plasma levels of interleukin(IL)-1β were elevated in the offspring of GDM dams. To determine whether this was related to immune reactivity, spleen cells from both the newborn and 16week-old offspring were isolated and reactivity to the toll-like receptor activators, pam3CSK4 and lipopolysaccharides were measured over a 72h timeframe. Spleen cells of GDM dams exhibited sustained stimulation of interleukin(IL)-1β and IL-10 production, whereas IL-1β and IL-10 synthesis diminished over time in spleen cells from the offspring of lean dams. Additive effects of GDM exposure and post-weaning HFS diet were not observed, suggesting the effects of GDM on cytokine production are independent of the post-weaning diet. Thus, we conclude that exposure to GDM in utero may condition the immune reactivity of spleen cells.
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Offspring from the gestational diabetes mellitus model exhibit a hyperactive immune response that manifests in neuroinflammation, which was correlated with cognitive impairment and reduced synaptic integrity.
“Further studies using this GDM model have demonstrated that the offspring exhibit a hyperactive immune response [108] that manifests in neuroinflammation, which was correlated with cognitive impairment and reduced synaptic integrity [109].”
From Recent Experimental Studies of Maternal Obesity, Diabetes during Pregnancy and the Developmental Origins of Cardiovascular Disease (2022) · cites this paper as [108]
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Cites
Rausch, M. E., Weisberg, S., Vardhana, P., & Tortoriello, D. V. (2008) · International Journal of Obesity · doi:10.1038/sj.ijo.0803744 · Cited by 585 · see citing works
Jayashree, B., Bibin, Y. S., Prabhu, D., Shanthirani, C. S., Gokulakrishnan, K., Lakshmi, B. S., Mohan, V., & Balasubramanyam, M. (2014) · Molecular and Cellular Biochemistry · doi:10.1007/s11010-013-1911-4 · Cited by 353 · see citing works
Vuong, B., Odero, G., Rozbacher, S., Stevenson, M., Kereliuk, S. M., Pereira, T. J., Dolinsky, V. W., & Kauppinen, T. M. (2017) · Journal of Neuroinflammation · doi:10.1186/s12974-017-0859-9 · Cited by 147 · see citing works
Self-citationMouralidarane, A., Soeda, J., Visconti-Pugmire, C., Samuelsson, A.-M., Pombo, J., Maragkoudaki, X., Butt, A., Saraswati, R., Novelli, M., Fusai, G., et al. (2013) · Hepatology · doi:10.1002/hep.26248 · Cited by 145 · see citing works
Alfaradhi, M. Z., Fernandez-Twinn, D. S., Martin-Gronert, M. S., Musial, B., Fowden, A., & Ozanne, S. E. (2014) · American Journal of Physiology-Regulatory, Integrative and Comparative Physiology · doi:10.1152/ajpregu.00049.2014 · Cited by 136 · see citing works
Flagged first, then by citations
Research works printed but not linked: 2
- D. Agarwal, R.B. Dange, J. Vila, A.J. Otamendi, J. Francis, Detraining differentially pre- served beneficial effects of exercise on hypertension: effects on blood pressure, car- diac function, brain inflammatory cytokines and oxidative stress, PLoS One 7 (2012), e52569.
- S. Hawkesworth, S.E. Moore, A.J. Fulford, G.R. Barclay, A.A. Darboe, H. Mark, O.A. Nyan, A.M. Prentice, Evidence for metabolic endotoxemia in obese and diabetic Gambian women, Nutr. Diabetes 3 (2013), e83.
Cited by
Sorted by citations
Vuong, B., Odero, G., Rozbacher, S., Stevenson, M., Kereliuk, S. M., Pereira, T. J., Dolinsky, V. W., & Kauppinen, T. M. (2017) · Journal of Neuroinflammation · doi:10.1186/s12974-017-0859-9 · Cited by 147 · see citing works
Self-citationKereliuk, S. M., & Dolinsky, V. W. (2022) · International Journal of Molecular Sciences · doi:10.3390/ijms23084467 · Cited by 34 · see citing works
“Further studies using this GDM model have demonstrated that the offspring exhibit a hyperactive immune response [108] that manifests in neuroinflammation, which was correlated with cognitive impairment and reduced synaptic integrity [109].”
Self-citationAgarwal, P., Brar, N., Morriseau, T. S., Kereliuk, S. M., Fonseca, M. A., Cole, L. K., Jha, A., Xiang, B., Hunt, K. L., Seshadri, N., et al. (2019) · Endocrinology · doi:10.1210/en.2019-00232 · Cited by 34 · see citing works
Self-citation
- Authors
- Li, Q., Pereira, T. J., Moyce, B. L., Mahood, T. H., Doucette, C. A., Rempel, J., & Dolinsky, V. W.
- Affiliations
- University of Manitoba (CA)
- Children's Hospital Research Institute of Manitoba (CA)
- Corresponding author
- Dolinsky, Vernon W.
- Venue
- Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease
- Publisher
- Elsevier BV
- Published
- 2016-11
- Volume
- 1862
- Issue
- 11
- Pages
- 2137-2146
- ISSN
- 0925-4439 (ISSN-L)
- OpenAlex
- W2508796801
- Cited by
- 18
- References
- 5
- Citations by year
- 2025: 4 · 2024: 2 · 2023: 3 · 2022: 2 · 2021: 1 · 2020: 1 · 2019: 1 · 2018: 1 · 2017: 3
- FWCI
- 2.15
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Children's Hospital Research Institute of Manitoba (OG 2012-04)
- Canadian Institutes of Health Research (MOP-136885)
- Topics
- Health Sciences › Medicine › Obstetrics and Gynecology › Gestational Diabetes Research and Management
- Health Sciences › Medicine › Pediatrics, Perinatology and Child Health › Birth, Development, and Health
- Life Sciences › Immunology and Microbiology › Immunology › Immune Response and Inflammation
- Keywords
- gestational diabetes mellitus, prenatal exposure, offspring, type 2 diabetes risk, insulin resistance, inflammation, IL-1β, IL-10, TLR4, TLR2, splenocytes, immunoreactivity, maternal diet, neonatal inflammation
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
