Cited by 2Microbial metabolism and enzyme functionMicrobial Fuel Cells and BioremediationBacterial Genetics and Biotechnology

The DOI matches its Crossref record; 43 of the 43 references it cites were found in a registry or an index.

Histidine betaine trimethylammonia-lyase, enzyme coupled with terminal urocanate reductase in Shewanella woodyi grown anaerobically

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Bertsova, Yulia V., Serebryakova, Marina V., Baykov, Alexander A., Bogachev, Alexander V.·2026·Biochimica et Biophysica Acta (BBA) - General Subjects

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Abstract

Bacteria coping with oxygen deficiency can switch to alternative terminal electron acceptors, which can be normal metabolic intermediates or products of dedicated coupled reactions. In the latter case, the genes for the respective terminal reductase and coupling enzyme are expected to cluster in the genome. Here, we determined the roles of two uncharacterized periplasmic proteins encoded by the swoo_3912-swoo_3913 gene cluster in the facultatively anaerobic marine bacterium Shewanella woodyi. We confirmed the current database annotation of the former protein as "urocanate reductase" but identified the latter protein as a histidine betaine trimethylammonia-lyase (HBTL). HBTL converts histidine betaine into urocanate and trimethylamine and is remarkably specific for histidine betaine as substrate. HBTL requires Mg 2+ for activity and undergoes slow reversible inactivation at low Mg 2+ concentrations. HBTL activity was not evident in S. woodyi cells grown aerobically but was induced in cells grown anaerobically. Both histidine betaine and urocanate supported anaerobic S. woodyi growth and, hence, respiration. Similar gene clusters are found in many anaerobic bacteria, suggesting a wide occurrence of the anaerobic respiration pathway discovered in this work in the bacterial world.

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Crossref · 10.1016/j.bbagen.2025.130886

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Metadata

Authors
Bertsova, Y. V., Serebryakova, M. V., Baykov, A. A., & Bogachev, A. V.
Affiliations
Lomonosov Moscow State University (RU)
Corresponding author
Bogachev, Alexander V.
Venue
Biochimica et Biophysica Acta (BBA) - General Subjects
Publisher
Elsevier BV
Published
2026-01
Volume
1870
Issue
1
Pages
130886
ISSN
0304-4165 (ISSN-L)
OpenAlex
W4416276307
Cited by
2
References
43
Citations by year
2026: 2
FWCI
0.60
Type
journal article
Language
en
Open access
Locations
  • Biochimica et Biophysica Acta (BBA) - General Subjects (publishedVersion)
  • bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion)
  • PubMed (publishedVersion)
Funders
Russian Science Foundation (24-24-00043)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial metabolism and enzyme function
  • Physical Sciences › Environmental Science › Environmental Engineering › Microbial Fuel Cells and Bioremediation
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
Keywords
anaerobic respiration, gene clusters, periplasmic proteins, trimethylamine, magnesium-dependent enzyme, enzyme inactivation, facultative anaerobes, marine bacteria
MeSH terms
Ammonia-Lyases, Anaerobiosis, Bacterial Proteins, Betaine, Multigene Family, Histidine, Methylamines, Oxidoreductases, Shewanella

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