CoChat
Sign Up
Cited by 53Fungal and yeast genetics researchPlant biochemistry and biosynthesisLipid metabolism and biosynthesis

A systematic study of yeast sterol biosynthetic protein-protein interactions using the split-Ubiquitin system

Mo, Caiqing, Bard, Martin·2005·Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids
DOI 10.1016/j.bbalip.2005.11.002
Open this paper in CoChat — free account

Abstract

Sterol biosynthesis occurs in the ER and most sterol biosynthetic enzymes have transmembrane domains. However, due to difficulties in characterizing membrane protein-protein interactions, the nature of the sterol biosynthetic complex as well as in vivo interactions between various enzymes have not been described. We employed a split-ubiquitin membrane protein yeast two-hybrid system to characterize interactions between sterol biosynthetic proteins. Fourteen bait constructs were co-transformed into a reporter yeast strain with 14 prey constructs representing all sterol enzymatic reactions beginning with the synthesis of squalene. Our results not only confirmed several previous interactions, but also allowed us to identify novel interactions. Based on these results, ergosterol biosynthetic enzymes display specific protein-protein interactions forming a functional complex we designate, the ergosome. In this complex, Erg11p, Erg25p, Erg27p, and Erg28p appear to form a core center that can interact with other enzymes in the pathway. Also Erg24p and Erg2p, two enzymes that are sensitive to morpholine antifungals, appear to interact with one another; however, the profile of protein interaction partners appears to be unique. Erg2p and Erg3p, two enzymes catalyzing sequential reactions also appear to have different interaction partners. Our results provide a working model as to how sterol biosynthetic enzymes are topologically organized not only in yeast but in plant and animal systems that share many of these biosynthetic reactions.

Integrity signals

Registry

Crossref · 10.1016/j.bbalip.2005.11.002

Cites

34 of 34 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

  • Multiple enzymes that participate in the late stage of ergosterol biosynthesis are transmembrane-containing proteins that associate into a functional complex, denoted the ergosome, to facilitate catalysis.

    “Multiple enzymes that participate in the late stage of ergosterol biosynthesis are transmembrane-containing proteins that associate into a functional complex, denoted the ergosome, to facilitate catalysis [53].”

    From Regulation of Ergosterol Biosynthesis in Saccharomyces cerevisiae (2020) · cites this paper as [53]

Cites

Flagged first, then by citations

Cited by

Sorted by citations

Related

Authors
Mo, C., & Bard, M.
Affiliations
Indiana University – Purdue University Indianapolis (US)
Corresponding author
Bard, Martin
Venue
Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids
Publisher
Elsevier BV
Published
2005-12
Volume
1737
Issue
2-3
Pages
152-160
ISSN
1388-1981 (ISSN-L)
DOI
10.1016/j.bbalip.2005.11.002
OpenAlex
W2038739996
Cited by
53
References
34
Citations by year
2026: 2 · 2025: 1 · 2024: 2 · 2022: 3 · 2021: 7 · 2020: 4 · 2019: 2 · 2018: 5 · 2017: 3 · 2016: 4 · 2015: 4 · 2014: 3 · 2013: 1 · 2012: 1
FWCI
0.63
Type
journal article
Language
en
Open access
closed
Licence
www.elsevier.com/tdm/userlicense/1.0/ (tdm)
Locations
  • Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • National Institutes of Health
  • National Institute of General Medical Sciences (R01GM062104, GM 62104)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Plant biochemistry and biosynthesis
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
Keywords
sterol biosynthesis, protein-protein interaction, ergosterol biosynthesis, membrane protein complexes, squalene synthesis, endoplasmic reticulum
MeSH terms
Base Sequence, DNA, Fungal, Ergosterol, Genes, Fungal, Lac Operon, Protein Binding, Saccharomyces cerevisiae, Sterols, Genes, Reporter, DNA Primers, Two-Hybrid System Techniques, Ubiquitin, Saccharomyces cerevisiae Proteins

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review