Lipopolysaccharide delays demyelination and promotes oligodendrocyte precursor proliferation in the central nervous system
Open accessAbstract
Systemic infection can influence the course in many diseases of the central nervous system (CNS) such as multiple sclerosis (MS), yet the relationship between infection outside the CNS and potential damage and/or protection within the CNS is still not understood. Activation of microglia is a characteristic feature of most CNS autoimmune disorders, including MS, and both protective and degenerative functions of microglia have been proposed. Hence, we analyzed the effects of a systemic inflammatory reaction induced by peripheral treatment with lipopolysaccharide (LPS) on microglial reaction and cuprizone induced de- and remyelination. We found that LPS administration delayed demyelination, which was linked with inhibition of microglial proliferation and reduced numbers of activated microglia. The phenotype of microglia changed as an increase of Toll-like receptor 4 was found. During remyelination, LPS treatment delayed the onset of myelin protein re-expression, but later there was a beneficial effect via an increase of proliferating oligodendrocyte precursor cells (OPC) and mature oligodendrocytes. Moreover, the expression of ciliary neurotrophic factor was increased in response to LPS, a growth factor known to mediate OPC proliferation. Additional experiments showed that the time window to induce LPS effects was limited and associated with the presence of microglia. In conclusion, LPS delayed demyelination and caused beneficial effects on remyelination via increasing the proli…
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Once demyelination is complete, the amount of activated RCA-1/Mac-3-positive microglia starts to decrease.
“Once demyelination is complete the amount of activated RCA-1 (RCA, Ricinus communis agglutinin-1)/Mac-3-positive microglia starts to decrease (Mason et al., 2004; Skripuletz et al., 2011b).”
From Glial response during cuprizone-induced de- and remyelination in the CNS: lessons learned (2014) · cites this paper as Skripuletz et al., 2011b
After 3 weeks of cuprizone treatment, mature oligodendrocytes are almost completely depleted.
“After 3 weeks of cuprizone treatment mature oligodendrocytes are almost completely depleted (Hesse et al., 2010; Skripuletz et al., 2011b).”
From Glial response during cuprizone-induced de- and remyelination in the CNS: lessons learned (2014) · cites this paper as Skripuletz et al., 2011b
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Praet, J., Guglielmetti, C., Berneman, Z., Van der Linden, A., & Ponsaerts, P. (2014) · Neuroscience & Biobehavioral Reviews · doi:10.1016/j.neubiorev.2014.10.004 · Cited by 435 · see citing works
Glial response during cuprizone-induced de- and remyelination in the CNS: lessons learned
Gudi, V., Gingele, S., Skripuletz, T., & Stangel, M. (2014) · Frontiers in Cellular Neuroscience · doi:10.3389/fncel.2014.00073 · Cited by 375 · see citing works
Self-citation
- Authors
- Skripuletz, T., Miller, E., Grote, L., Gudi, V., Pul, R., Voss, E., Škuljec, J., Moharregh-Khiabani, D., Trebst, C., & Stangel, M.
- Affiliations
- Medizinische Hochschule Hannover (DE)
- Corresponding author
- Stangel, Martin
- Venue
- Brain, Behavior, and Immunity
- Publisher
- Elsevier BV
- Published
- 2011-11
- Volume
- 25
- Issue
- 8
- Pages
- 1592-1606
- ISSN
- 0889-1591 (ISSN-L)
- OpenAlex
- W2002764553
- Cited by
- 31
- References
- 7
- Citations by year
- 2026: 1 · 2025: 1 · 2024: 2 · 2023: 1 · 2022: 2 · 2021: 1 · 2020: 1 · 2019: 1 · 2018: 2 · 2017: 4 · 2015: 6 · 2014: 5 · 2013: 2 · 2012: 2
- FWCI
- 2.02
- Type
- journal article
- Language
- en
- Open access
- green
- zenodo.org
- Licence
- Locations
- Brain Behavior and Immunity (publishedVersion)
- PubMed (publishedVersion)
- Zenodo (CERN European Organization for Nuclear Research) (submittedVersion, other-oa)
- Funders
- Medizinischen Hochschule Hannover
- Topics
- Health Sciences › Medicine › Pathology and Forensic Medicine › Multiple Sclerosis Research Studies
- Life Sciences › Neuroscience › Neurology › Neuroinflammation and Neurodegeneration Mechanisms
- Life Sciences › Neuroscience › Developmental Neuroscience › Neurogenesis and neuroplasticity mechanisms
- Keywords
- lipopolysaccharide, demyelination, remyelination, oligodendrocyte precursor cells, microglia, TLR4, ciliary neurotrophic factor, cuprizone, multiple sclerosis, systemic infection, oligodendrocyte proliferation
- MeSH terms
- Animals, Axons, Cell Count, Central Nervous System, Chelating Agents, Cuprizone, Demyelinating Diseases, Flow Cytometry, Immunohistochemistry, Lipopolysaccharides, Male, Mice, Inbred C57BL, Myelin Sheath, Oligodendroglia, Phagocytosis, Rats, Sprague-Dawley, Microglia, Cell Proliferation, Mice, Rats, Neural Stem Cells, Real-Time Polymerase Chain Reaction
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