The ITIM-bearing CLECSF6 (DCIR) is down-modulated in neutrophils by neutrophil activating agents
Abstract
The recently discovered CLECSF6 protein displays the features of a receptor involved in the down-modulation of leukocyte activation. Although CLECSF6 has been the focus of the interest of many researchers lately, a Western blot characterization of the protein is still lacking. This highly reduces our ability to gain full knowledge of the biological relevance of this protein in cell responses. We produced two rabbit polyclonal antisera that detected a glycosylated protein at approximately 35kDa in neutrophils. Four different CLECSF6 mRNA species have been discovered to date. When deglycosylated, the protein displayed the molecular weight expected for the longest CLECSF6 form. Neutrophil membrane fractions were strongly enriched in the protein. We showed a down-modulation of the expression of this protein in neutrophils treated with granulocyte-macrophage-colony stimulating factor (GM-CSF), tumor necrosis factor (TNF-alpha), lipopolysaccharide (LPS), and interleukin (IL)-4. This work supports the hypothesis that CLECSF6 is involved in the control of inflammation in neutrophils.
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IL1R1 and IL15 were strongly co-expressed with the Mac-1 complex and with CLEC4A.
“To the same group of pro-inflammatory molecules belong also interleukin (IL)1 receptor type I (IL1R1), which modulates many cytokine induced immune and inflammatory responses, and IL15 (IL15), which regulates T and natural killer cell activation and proliferation [31,32]. Both of them were strongly co-expressed with the Mac-1 complex and with C-type lectin domain family 4 member A (CLEC4A), known”
From Adipose tissue transcriptomic signature highlights the pathological relevance of extracellular matrix in human obesity (2008) · cites this paper as [33]
Cites
Bradford, M. M. (1976) · Analytical Biochemistry · doi:10.1016/0003-2697(76)90527-3 · Cited by 255,302 · see citing works
Molecular Cloning: A Laboratory Manual
Sambrook, J., Fritsch, E., & Maniatis, T. (1989) · UCLA - Biblioteca de Ciencias de la Salud · Cited by 133,589 · see citing works
Molecular cloning: A laboratory manual
Deininger, P. L. (1990) · Analytical Biochemistry · doi:10.1016/0003-2697(90)90595-z · Cited by 84,684 · see citing works
Bates, E. E. M., Fournier, N., Garcia, E., Valladeau‐Guilemond, J., Durand, I., Pin, J., Zurawski, S., Patel, S., Abrams, J. S., Lebecque, S., et al. (1999) · The Journal of Immunology · doi:10.4049/jimmunol.163.4.1973 · Cited by 269 · see citing works
Vely, F., & Vivier, E. D. (1997) · The Journal of Immunology · doi:10.4049/jimmunol.159.5.2075 · Cited by 160 · see citing works
Hollister, J. R., Grabenhorst, E., Nimtz, M., Conradt, H. S., & Jarvis, D. L. (2002) · Biochemistry · doi:10.1021/bi026455d · Cited by 154 · see citing works
Kanazawa, N., Okazaki, T., Nishimura, H., et al. (2002) · Journal of Investigative Dermatology · doi:10.1046/j.0022-202x.2001.01633.x · Cited by 102 · see citing works
Isakov, N. (1997) · Immunologic Research · doi:10.1007/bf02786325 · Cited by 94 · see citing works
Nishiu, M., Yanagawa, R., Nakatsuka, S., Yao, M., Tsunoda, T., Nakamura, Y., & Aozasa, K. (2002) · Japanese Journal of Cancer Research · doi:10.1111/j.1349-7006.2002.tb01335.x · Cited by 43 · see citing works
Huang, X., Yuan, Z., Chen, G., et al. (2001) · Biochemical and Biophysical Research Communications · doi:10.1006/bbrc.2001.4322 · Cited by 36 · see citing works
Richard, M., Veilleux, P., Rouleau, M., Paquin, R., & Beaulieu, A. D. (2002) · Journal of Leukocyte Biology · doi:10.1189/jlb.71.5.871 · Cited by 36 · see citing works
Self-citationThibault, N., Harbour, D., Borgeat, P., Naccache, P. H., & Bourgoin, S. G. (2000) · Blood · doi:10.1182/blood.v95.2.519 · Cited by 26 · see citing works
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Henegar, C., Tordjman, J., Achard, V., Lacasa, D., Cremer, I., Guerre-Millo, M., Poitou, C., Basdevant, A., Stich, V., Viguerie, N., et al. (2008) · Genome Biology · doi:10.1186/gb-2008-9-1-r14 · Cited by 471 · see citing works
- Authors
- Richard, M., Thibault, N., Veilleux, P., Breton, R., & Beaulieu, A. D.
- Affiliations
- Centre hospitalier de l'Université Laval (CA)
- Corresponding author
- Beaulieu, André D
- Venue
- Biochemical and Biophysical Research Communications
- Publisher
- Elsevier BV
- Published
- 2003-10
- Volume
- 310
- Issue
- 3
- Pages
- 767-773
- ISSN
- 0006-291X (ISSN-L)
- OpenAlex
- W2052482830
- Cited by
- 30
- References
- 12
- Citations by year
- 2025: 1 · 2020: 1 · 2015: 3 · 2014: 1 · 2013: 5 · 2012: 2
- FWCI
- 0.65
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Biochemical and Biophysical Research Communications (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Immunology and Microbiology › Immunology › Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Life Sciences › Immunology and Microbiology › Immunology › Inflammation biomarkers and pathways
- Life Sciences › Immunology and Microbiology › Immunology › Immune Response and Inflammation
- Keywords
- DCIR, neutrophils, leukocyte activation, GM-CSF, TNF-α, lipopolysaccharide, IL-4, protein glycosylation, membrane protein expression, inflammation control, polyclonal antisera
- MeSH terms
- Animals, Cell Line, Cell Membrane, Electrophoresis, Polyacrylamide Gel, Genetic Vectors, Glycosylation, Humans, Insecta, Leukocytes, Lipopolysaccharides, Membrane Glycoproteins, Neutrophils, Peptides, Rabbits, Receptors, Immunologic, Recombinant Proteins, RNA, Messenger, Tumor Necrosis Factor-alpha, Immunoblotting, Blotting, Western, Down-Regulation, Interleukin-4, Cytokines, Baculoviridae, DNA, Complementary, Lectins, C-Type
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