Identification of two novel markers for alveolar epithelial type I and II cells
Abstract
Alveolar epithelial type I and type II cells (AEC I and II) are closely aligned in alveolar surface. There is much interest in the precise identification of AEC I and II in order to separate and evaluate functional and other properties of these two cells. This study aims to identify specific AEC I and AEC II cell markers by DNA microarray using the in vitro trans-differentiation of AEC II into AEC I-like cells as a model. Quantitative real-time PCR confirmed five AEC I genes: fibroblast growth factor receptor-activating protein 1, aquaporin 5, purinergic receptor P2X 7 (P2X7), interferon-induced protein, and Bcl2-associated protein, and one AEC II gene: gamma-aminobutyric acid receptor pi subunit (GABRP). Immunostaining on cultured cells and rat lung tissue indicated that GABRP and P2X7 proteins were specifically expressed in AEC II and AEC I, respectively. In situ hybridization of rat lung tissue confirmed the localization of GABRP mRNA in type II cells. P2X7 and GABRP identified in this study could be used as potential AEC I and AEC II markers for studying lung epithelial cell biology and monitoring lung injury.
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The P2X7 receptor is widely expressed through the body, including in immune, lung, and CNS cells, mainly microglia and oligodendrocytes.
“The P2X7 receptor is widely expressed through the body [47], including in immune [48], lung [49], and CNS cells, mainly microglia and oligodendrocytes [50–52], whereas its expression in neurons and astrocytes is still under discussion [50, 51, 53–60].”
From Hyperactivation of P2X7 receptors as a culprit of COVID-19 neuropathology (2021) · cites this paper as [49]
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- Authors
- Chen, Z., Jin, N., Narasaraju, T., Chen, J., McFarland, L. R., Scott, M., & Liu, L.
- Affiliations
- Oklahoma State University (US)
- Corresponding author
- Liu, Lin
- Venue
- Biochemical and Biophysical Research Communications
- Publisher
- Elsevier BV
- Published
- 2004-07
- Volume
- 319
- Issue
- 3
- Pages
- 774-780
- ISSN
- 0006-291X (ISSN-L)
- OpenAlex
- W2052250424
- Cited by
- 87
- References
- 31
- Citations by year
- 2026: 2 · 2025: 1 · 2024: 3 · 2023: 2 · 2022: 1 · 2021: 3 · 2020: 3 · 2019: 5 · 2018: 1 · 2017: 4 · 2016: 4 · 2015: 6 · 2014: 7 · 2013: 5 · 2012: 8
- FWCI
- 2.95
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Biochemical and Biophysical Research Communications (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Heart, Lung, and Blood Institute (R01HL071628, R01HL052146)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Physiology › Adenosine and Purinergic Signaling
- Health Sciences › Medicine › Pulmonary and Respiratory Medicine › Neonatal Respiratory Health Research
- Health Sciences › Medicine › Pulmonary and Respiratory Medicine › Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Keywords
- alveolar epithelial type I cells, alveolar type II cells, cell markers, P2X7 receptor, aquaporin 5, FGF19, interferon-induced proteins, transdifferentiation, lung injury, lung epithelium
- MeSH terms
- Animals, Cells, Cultured, Epithelial Cells, Immunohistochemistry, Male, Pulmonary Alveoli, Biomarkers, Rats, Sprague-Dawley, In Situ Hybridization, Receptors, Purinergic P2, Receptors, GABA, Oligonucleotide Array Sequence Analysis, Respiratory Mucosa, Gene Expression Profiling, Protein Subunits, Rats, Receptors, Purinergic P2X7
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