Differentiation of human adipose stromal cells into hepatic lineage in vitro and in vivo
Abstract
Embryonic stem cells (ES cells), bone marrow-derived mesenchymal stem cells, umbilical cord blood-derived mesenchymal stem cells, and hepatic stem cells in liver have been known as a useful source that can induce to differentiate into hepatocytes. In this study, we examined whether human adipose tissue-derived stromal cells (hADSC) can differentiate into hepatic lineage in vitro. hADSC, that were induced to differentiate into hepatocyte-like cells by the treatment of HGF and OSM, had morphology similar to hepatocytes. Addition of DMSO enhanced differentiation into hepatocytes. RT-PCR and immunocytochemical analysis showed that hADSC express albumin and alpha-fetoprotein during differentiation. Differentiated hADSC showed LDL uptake and production of urea. Additionally, transplanted hADSC to CCl4-injured SCID mouse model were able to be differentiated into hepatocytes and they expressed albumin in vivo. Mesenchymal stem cells isolated from human adipose tissue are immunocompatible and are easily isolated. Therefore, hADSC may become an alternative source to hepatocyte regeneration or liver cell transplantation.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
MSCs derived from various sources can differentiate into functional HLCs along the endodermal lineage under both in vitro and in vivo conditions in experimental animal models and humans.
“It has been reported in experimental animal models and humans that MSCs derived from various sources can differentiate into functional HLCs along the endodermal lineage under both in vitro and in vivo conditions [90–92].”
From Mechanisms, efficacy, and future perspectives of cellular-based therapies for liver fibrosis/cirrhosis: focusing on mesenchymal stromal cells (2025) · cites this paper as [92]
Cited by
Sorted by citations
Kern, S., Eichler, H., Stoeve, J., Klüter, H., & Bieback, K. (2006) · STEM CELLS · doi:10.1634/stemcells.2005-0342 · Cited by 3,370 · see citing works
Adipose-derived stem cells for regenerative medicine
Gimble, J. M., Katz, A. J., & Bunnell, B. A. (2007) · Circulation Research · doi:10.1161/01.res.0000265074.83288.09 · Cited by 2,332 · see citing works
Comparative Analysis of Mesenchymal Stem Cells from Bone Marrow, Cartilage, and Adipose Tissue
Peng, L., Jia, Z., Yin, X., Zhang, X., Liu, Y., Chen, P., Ma, K., & Zhou, C. (2008) · Stem Cells and Development · doi:10.1089/scd.2007.0217 · Cited by 368 · see citing works
Tissue-resident stem cells promote breast cancer growth and metastasis
Muehlberg, F. L., Song, Y.-H., Krohn, A., Pinilla, S. P., Droll, L. H., Leng, X., Seidensticker, M., Ricke, J., Altman, A. M., Devarajan, E., et al. (2009) · Carcinogenesis · doi:10.1093/carcin/bgp036 · Cited by 330 · see citing works
Human Adipose Stem Cells: From Bench to Bedside
De Francesco, F., Ricci, G., D'Andrea, F., Nicoletti, G. F., & Ferraro, G. A. (2015) · Tissue Engineering Part B: Reviews · doi:10.1089/ten.teb.2014.0608 · Cited by 163 · see citing works
Jansen, B. J. H., Gilissen, C., Roelofs, H., Schaap-Oziemlak, A., Veltman, J. A., Raymakers, R. A. P., Jansen, J. H., Kögler, G., Figdor, C. G., Torensma, R., et al. (2010) · Stem Cells and Development · doi:10.1089/scd.2009.0288 · Cited by 139 · see citing works
Quirici, N., Scavullo, C., de Girolamo, L., Lopa, S., Arrigoni, E., Deliliers, G. L., & Brini, A. T. (2010) · Stem Cells and Development · doi:10.1089/scd.2009.0408 · Cited by 117 · see citing works
Regenerative therapy after cancer: what are the risks?
Donnenberg, V. S., Zimmerlin, L., Rubin, J. P., & Donnenberg, A. D. (2010) · Tissue Engineering Part B: Reviews · doi:10.1089/ten.teb.2010.0352 · Cited by 95 · see citing works
Stem Cell-Based Strategies: The Future Direction of Bioartificial Liver Development
Feng, L., Wang, Y., Fu, Y., Li, T., & He, G. (2024) · Stem Cell Reviews and Reports · doi:10.1007/s12015-023-10672-5 · Cited by 9 · see citing works
Pan, X., Gao, T., & Wang, B. (2025) · Cell & Bioscience · doi:10.1186/s13578-025-01520-9 · Cited by 2 · see citing works
“It has been reported in experimental animal models and humans that MSCs derived from various sources can differentiate into functional HLCs along the endodermal lineage under both in vitro and in vivo conditions [90–92].”
- Authors
- Seo, M. J., Suh, S. Y., Bae, Y. C., & Jung, J. S.
- Affiliations
- Pusan National University (KR)
- Dong-A University (KR)
- Corresponding author
- Jung, Jin Sup
- Venue
- Biochemical and Biophysical Research Communications
- Publisher
- Elsevier BV
- Published
- 2005-03
- Volume
- 328
- Issue
- 1
- Pages
- 258-264
- ISSN
- 0006-291X (ISSN-L)
- OpenAlex
- W2118458879
- Cited by
- 530
- References
- 0
- Citations by year
- 2026: 1 · 2025: 3 · 2024: 12 · 2023: 9 · 2022: 13 · 2021: 10 · 2020: 18 · 2019: 22 · 2018: 22 · 2017: 19 · 2016: 20 · 2015: 31 · 2014: 39 · 2013: 44 · 2012: 40
- FWCI
- 15.19
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Biochemical and Biophysical Research Communications (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Genetics › Mesenchymal stem cell research
- Health Sciences › Medicine › Hepatology › Liver physiology and pathology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Pluripotent Stem Cells Research
- Keywords
- adipose-derived stromal cells, hepatocyte differentiation, hepatocyte-like cells, hepatocyte regeneration, hepatocyte transplantation, hepatocyte growth factor, albumin, alpha-fetoprotein, LDL uptake, urea synthesis, carbon tetrachloride-induced liver injury, SCID mice, mesenchymal stem cells
- MeSH terms
- Adult, Animals, Cell Differentiation, Dimethyl Sulfoxide, Female, Humans, Lipoproteins, LDL, Male, Middle Aged, Peptides, Urea, Mice, SCID, Mice, Inbred NOD, Stromal Cells, Hepatocyte Growth Factor, Adipocytes, Hepatocytes, Cell Size, Mice, Oncostatin M
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
