Cited by 83Cancer, Hypoxia, and MetabolismCancer and biochemical researchRedox biology and oxidative stress

High activity of mitochondrial glycerophosphate dehydrogenase and glycerophosphate-dependent ROS production in prostate cancer cell lines

Chowdhury, Subir K.R., Gemin, Adam, Singh, Gurmit·2005·Biochemical and Biophysical Research Communications

Abstract

Most malignant cells are highly glycolytic and produce high levels of reactive oxygen species (ROS) compared to normal cells. Mitochondrial glycerophosphate dehydrogenase (mGPDH) participates in the reoxidation of cytosolic NADH by delivering reducing equivalents from this molecule into the electron transport chain, thus sustaining glycolysis. Here, we investigate the role of mGPDH in maintaining an increased rate of glycolysis and evaluate glycerophosphate-dependent ROS production in prostate cancer cell lines (LNCaP, DU145, PC3, and CL1). Immunoblot, polarographic, and spectrophotometric analyses revealed that mGPDH abundance and activity was significantly elevated in prostate cancer cell lines when compared to the normal prostate epithelial cell line PNT1A. Furthermore, both the glycolytic capacity and glycerophosphate-dependent ROS production was increased 1.68- to 4.44-fold and 5- to 7-fold, respectively, in prostate cancer cell lines when compared to PNT1A cells. Overall, these data demonstrate that mGPDH is involved in maintaining a high rate of glycolysis and is an important site of electron leakage leading to ROS production in prostate cancer cells.

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Crossref · 10.1016/j.bbrc.2005.06.017

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  • Increased GPDH activity has been detected in prostate cancer cells, insulinomas, and carcinoid tumors.

    “In fact, increased activity of GPDH has been detected in prostate cancer cells, insulinomas and carcinoid tumors (Chowdhury et al., 2005; MacDonald et al., 1990).”

    From The emerging hallmarks of cancer metabolism (2016) · cites this paper as Chowdhury et al., 2005

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Authors
Chowdhury, S. K. R., Gemin, A., & Singh, G.
Affiliations
Juravinski Cancer Centre (CA)
Corresponding author
Singh, Gurmit
Venue
Biochemical and Biophysical Research Communications
Publisher
Elsevier BV
Published
2005-08
Volume
333
Issue
4
Pages
1139-1145
ISSN
0006-291X (ISSN-L)
OpenAlex
W2092715210
Cited by
83
References
38
Citations by year
2026: 2 · 2025: 7 · 2024: 4 · 2023: 3 · 2022: 4 · 2021: 4 · 2020: 1 · 2019: 2 · 2018: 3 · 2017: 2 · 2016: 2 · 2015: 3 · 2014: 4 · 2013: 4 · 2012: 6
FWCI
2.31
Type
journal article
Language
en
Open access
closed
Locations
  • Biochemical and Biophysical Research Communications (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • Canadian Institutes of Health Research
  • Congressionally Directed Medical Research Programs
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Cancer, Hypoxia, and Metabolism
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Cancer and biochemical research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Redox biology and oxidative stress
Keywords
prostate cancer, reactive oxygen species, glycolysis, electron transport chain, NADH reoxidation, electron leakage, cancer metabolism
MeSH terms
Enzyme Activation, Glycerolphosphate Dehydrogenase, Glycerophosphates, Glycolysis, Humans, Male, Mitochondria, Oxygen, Oxygen Consumption, Prostatic Neoplasms, Reactive Oxygen Species, Cell Line, Tumor

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