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Cited by 49Kruppel-like factors researchImmune Response and InflammationCytokine Signaling Pathways and Interactions

KLF4 regulates the expression of interleukin-10 in RAW264.7 macrophages

Liu, Junwen, Zhang, Huali, Liu, Ying, Wang, Kangkai, Feng, Yansheng, Liu, Meidong, Xiao, Xianzhong·2007·Biochemical and Biophysical Research Communications
DOI 10.1016/j.bbrc.2007.07.157
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Abstract

Krüppel-like factor 4 (KLF4) is an evolutionarily conserved zinc finger-containing transcription factor. In the current study, RAW264.7 murine macrophages were treated with Escherichia coli lipopolysaccharide (LPS) to determine the expression of KLF4. A full-length cDNA or antisense oligonucleotides of KLF4 was transfected into RAW264.7 macrophages, and the expression and release of IL-10 were analyzed by RT-PCR and ELISA. The transcription and DNA binding activities of KLF4 to the IL-10 promoter were detected by the luciferase reporter and electrophoretic mobility shift assays. The results showed that treatment of RAW264.7 macrophages with LPS resulted in a dose- and time-dependent increase in KLF4 protein levels; KLF4 overexpression increased the expression of IL-10, while KLF4 inhibition decreased the expression of IL-10. The overexpression of KLF4 promoted the transcription and DNA binding activities of KLF4 to the IL-10 promoter. These results indicate that KLF4 plays an important role in regulating the expression of IL-10.

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Crossref · 10.1016/j.bbrc.2007.07.157

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  • KLF4 drives M2 polarization and regulates the expression of interleukin-10, and is targeted by miR-26a.

    “KLF4, a transcription factor that drives M2 polarization and regulates the expression of interleukin-10, is a crucial factor of the polarization course [199] and is targeted by miR-26a.”

    From MicroRNAs as Regulators of Phagocytosis (2022) · cites this paper as [199]

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Authors
Liu, J., Zhang, H., Liu, Y., Wang, K., Feng, Y., Liu, M., & Xiao, X.
Affiliations
Central South University (CN)
Corresponding author
Xiao, Xianzhong
ORCID
Venue
Biochemical and Biophysical Research Communications
Publisher
Elsevier BV
Published
2007-10
Volume
362
Issue
3
Pages
575-581
ISSN
0006-291X (ISSN-L)
DOI
10.1016/j.bbrc.2007.07.157
OpenAlex
W2030796291
Cited by
49
References
1
Citations by year
2025: 5 · 2023: 2 · 2022: 3 · 2021: 4 · 2019: 2 · 2018: 2 · 2017: 3 · 2016: 4 · 2015: 5 · 2013: 2 · 2012: 6
FWCI
1.06
Type
journal article
Language
en
Open access
closed
Licence
Locations
  • Biochemical and Biophysical Research Communications (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • National Natural Science Foundation of China (30330280, 30400169, 30571746, 30571846)
  • Ministry of Education of the People's Republic of China (20060533009)
  • National Key Research and Development Program of China (2007CB512007)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Kruppel-like factors research
  • Life Sciences › Immunology and Microbiology › Immunology › Immune Response and Inflammation
  • Health Sciences › Medicine › Oncology › Cytokine Signaling Pathways and Interactions
Keywords
KLF4, IL-10, macrophages, RAW 264.7 macrophages, lipopolysaccharide, transcriptional regulation, IL-10 promoter, zinc finger transcription factor, anti-inflammatory gene expression
MeSH terms
Kruppel-Like Factor 4, Animals, Cell Line, Cell Nucleus, Dose-Response Relationship, Drug, Gene Expression Regulation, Luciferases, Macrophages, Oligonucleotides, Promoter Regions, Genetic, Transcription, Genetic, Transfection, Interleukin-10, DNA, Complementary, Mice, Kruppel-Like Transcription Factors

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