Is Kir6.1 a subunit of mitoKATP?
Open accessAbstract
The subunit composition of the mitochondrial ATP-sensitive K(+)-channel (mitoK(ATP)) is unknown, though some suspect a role for the inward rectifier, Kir6.1, based largely on antibody studies of heart mitochondria. To ascertain the molecular identity of mitoK(ATP) we therefore sought to purify this putative mitochondrial Kir6.1, and conclusively identify the subunits by mass spectrometry. Immunoblots, conducted with two commercially available antibodies, revealed two distinct signals in isolated heart mitochondria, of 51 and 48kDa, respectively. Localization was confirmed by either immuno-gold electron microscopy or by immunofluorescence. Each putative Kir6.1 species was extracted, purified, and identified by LC-MS/MS. The 51kDa band was identified as NADH-dehydrogenase flavoprotein 1, while the preponderant protein in the 48-kDa band was mitochondrial isocitrate dehydrogenase (NADP form). 1D-, 2D-, and native gel analyses were consistent with these assignments. The data suggest it is premature to assign Kir6.1 a role in mitoK(ATP) on the basis of immunoreactivity alone.
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KATP channel subunits could not be identified in highly purified mitochondria, challenging the existence of mitoKATP.
“Especially, the fact that the K ATP channel subunits could not be identified so far in highly purified mitochondria challenges its existence [145,146].”
From Differential effects of complex II on mitochondrial ROS production and their relation to cardioprotective pre- and postconditioning (2013) · cites this paper as [146]
Cites
Mass spectrometric sequencing of proteins silver-stained polyacrylamide gels.
Shevchenko, A., Wilm, M., Vorm, O., & Mann, M. F. (1996) · Analytical Chemistry · doi:10.1021/ac950914h · Cited by 9,047 · see citing works
A TECHNIQUE FOR ULTRACRYOTOMY OF CELL SUSPENSIONS AND TISSUES
Tokuyasu, K. T. (1973) · The Journal of Cell Biology · doi:10.1083/jcb.57.2.551 · Cited by 1,080 · see citing works
Cardioprotective Effect of Diazoxide and Its Interaction With Mitochondrial ATP-Sensitive K+Channels
Garlid, K., Pauček, P., Yarov-Yarovoy, V. M., Murray, H. N., Darbenzio, R. B., DʼAlonzo, A. J., Lodge, N. J., Smith, M. A., & Grover, G. J. (1997) · Circulation Research · doi:10.1161/01.res.81.6.1072 · Cited by 1,019 · see citing works
Blockade of ATP-sensitive potassium channels prevents myocardial preconditioning in dogs.
Gross, G. J., & Auchampach, J. A. (1992) · Circulation Research · doi:10.1161/01.res.70.2.223 · Cited by 953 · see citing works
KATP channels as molecular sensors of cellular metabolism
Nichols, C. G. (2006) · Nature · doi:10.1038/nature04711 · Cited by 849 · see citing works
Mitochondrial ATP-Dependent Potassium Channels
Liu, Y., Sato, T., O'Rourke, B. P., & Marbán, E. (1998) · Circulation · doi:10.1161/01.cir.97.24.2463 · Cited by 816 · see citing works
Self-citationATP-sensitive K+ channel in the mitochondrial inner membrane
Inoue, I., Nagase, H., Kishi, K., & Higuti, T. (1991) · Nature · doi:10.1038/352244a0 · Cited by 751 · see citing works
ATP-sensitive potassium channelopathies: focus on insulin secretion
Ashcroft, F. M. (2005) · Journal of Clinical Investigation · doi:10.1172/jci25495 · Cited by 627 · see citing works
[16] Isolation of subcellular organelles
Storrie, B., & Amadden, E. (1990) · Methods in Enzymology · doi:10.1016/0076-6879(90)82018-w · Cited by 555 · see citing works
The Mitochondrial K Channel as a Receptor for Potassium Channel Openers
Garlid, K., Pauček, P., Yarov-Yarovoy, V. M., Sun, X., & Schindler, P. A. (1996) · Journal of Biological Chemistry · doi:10.1074/jbc.271.15.8796 · Cited by 445 · see citing works
Evidence for Mitochondrial K + Channels and Their Role in Cardioprotection
O'Rourke, B. P. (2004) · Circulation Research · doi:10.1161/01.res.0000117583.66950.43 · Cited by 444 · see citing works
Recovery of brain mitochondrial function in the rat after complete and incomplete cerebral ischemia.
Rehncrona, S., Mela, L., & Siesjö, B. K. (1979) · Stroke · doi:10.1161/01.str.10.4.437 · Cited by 382 · see citing works
HANLEY, P., & DAUT, J. (2005) · Journal of Molecular and Cellular Cardiology · doi:10.1016/j.yjmcc.2005.04.002 · Cited by 218 · see citing works
Cardiac K channels in health and disease
Kane, G. C., Liu, X., Yamada, S., Olson, T. M., & Terzic, A. (2005) · Journal of Molecular and Cellular Cardiology · doi:10.1016/j.yjmcc.2005.02.026 · Cited by 196 · see citing works
Kir6.1: A Possible Subunit of ATP-Sensitive K+Channels in Mitochondria
Suzuki, M., Kotake, K., Fujikura, K., Inagaki, N., Suzuki, T., Gonoi, T., Seino, S., & Takata, K. (1997) · Biochemical and Biophysical Research Communications · doi:10.1006/bbrc.1997.7891 · Cited by 151 · see citing works
Liu, Y., Ren, G., O'Rourke, B. P., et al. (2001) · Molecular Pharmacology · doi:10.1124/mol.59.2.225 · Cited by 143 · see citing works
Seharaseyon, J., Ohler, A., Sasaki, N., et al. (2000) · Journal of Molecular and Cellular Cardiology · doi:10.1006/jmcc.2000.1226 · Cited by 98 · see citing works
Heart mitochondria contain functional ATP-dependent K+ channels
Lacza, Z. (2003) · Journal of Molecular and Cellular Cardiology · doi:10.1016/s0022-2828(03)00249-9 · Cited by 92 · see citing works
Lamping, K. G., & Gross, G. J. (1985) · Journal of Cardiovascular Pharmacology · doi:10.1097/00005344-198501000-00026 · Cited by 88 · see citing works
Lacza, Z., Snipes, J. A., Kis, B., Szabó, C., Grover, G. J., & Busija, D. W. (2003) · Brain Research · doi:10.1016/j.brainres.2003.09.046 · Cited by 87 · see citing works
Singh, H. (2003) · Journal of Molecular and Cellular Cardiology · doi:10.1016/s0022-2828(03)00041-5 · Cited by 86 · see citing works
Effects of Nicorandil and Nifedipine on Protection of Ischemic Myocardium
Lamping, K. G., CHRISTENSEN, C. W., Pelc, L. R., et al. (1984) · Journal of Cardiovascular Pharmacology · doi:10.1097/00005344-198405000-00024 · Cited by 65 · see citing works
Purification of pig heart mitochondrial membranes
Maı̈sterrena, B., Comte, J., & Gautheron, D. C. (1974) · Biochimica et Biophysica Acta (BBA) - Biomembranes · doi:10.1016/0005-2736(74)90036-4 · Cited by 52 · see citing works
Jiang, M. T., Ljubković, M., Nakae, Y., Shi, Y., Kwok, W., Stowe, D. F., & Bosnjak, Z. J. (2005) · American Journal of Physiology-Heart and Circulatory Physiology · doi:10.1152/ajpheart.01084.2005 · Cited by 36 · see citing works
Subunit composition of ATP-sensitive potassium channels in mitochondria of rat hearts
Cuong, D. V., Kim, N., Joo, H., Youm, J. B., Chung, J., Lee, Y.-S., Park, W. S., Kim, E., Park, Y. S., & Han, J. (2005) · Mitochondrion · doi:10.1016/j.mito.2004.12.001 · Cited by 35 · see citing works
Kuniyasu, A., Kaneko, K., Kawahara, K., et al. (2003) · FEBS Letters · doi:10.1016/s0014-5793(03)00936-0 · Cited by 28 · see citing works
Daban, J. (2001) · Electrophoresis · doi:10.1002/1522-2683()22:5 · Cited by 26 · see citing works
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Dröse, S. (2013) · Biochimica et Biophysica Acta (BBA) - Bioenergetics · doi:10.1016/j.bbabio.2013.01.004 · Cited by 201 · see citing works
Wojtovich, A. P., & Brookes, P. S. (2009) · Basic Research in Cardiology · doi:10.1007/s00395-009-0001-y · Cited by 107 · see citing works
- Authors
- Brian Foster, D., Rucker, J. J., & Marbán, E.
- Affiliations
- Johns Hopkins University (US)
- Cedars-Sinai Medical Center (US)
- Corresponding author
- Marbán, Eduardo
- ORCID
- Marbán, Eduardo
- Venue
- Biochemical and Biophysical Research Communications
- Publisher
- Elsevier BV
- Published
- 2008-02
- Volume
- 366
- Issue
- 3
- Pages
- 649-656
- ISSN
- 0006-291X (ISSN-L)
- PMC
- PMC2276631
- OpenAlex
- W1994968070
- Cited by
- 71
- References
- 27
- Citations by year
- 2026: 1 · 2023: 1 · 2019: 1 · 2018: 4 · 2017: 2 · 2016: 1 · 2015: 4 · 2014: 7 · 2013: 14 · 2012: 10
- FWCI
- 3.56
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Biochemical and Biophysical Research Communications (publishedVersion)
- PubMed (publishedVersion)
- PubMed Central (acceptedVersion)
- Funders
- National Heart, Lung, and Blood Institute (P01HL081427)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Mitochondrial Function and Pathology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Ion channel regulation and function
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › ATP Synthase and ATPases Research
- Keywords
- Kir6.1, mitochondrial ATP-sensitive potassium channel, inwardly rectifying potassium channels, subunit composition, heart mitochondria, antibody cross-reactivity, protein purification, mass spectrometry identification
- MeSH terms
- Amino Acid Sequence, Animals, Cells, Cultured, Mitochondria, Heart, Molecular Sequence Data, Potassium Channels, Protein Subunits, Potassium Channels, Inwardly Rectifying, KATP Channels
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