Cited by 59Chemotherapy-induced organ toxicity mitigationOral health in cancer treatmentChemotherapy-related skin toxicity

Minocycline attenuates 5-fluorouracil-induced small intestinal mucositis in mouse model

Huang, Tien-Yu, Chu, Heng-Cheng, Lin, Yi-Ling, Ho, Whae-Hong, Hou, Hsien-San, Chao, You-Chen, Liao, Ching-Len·2009·Biochemical and Biophysical Research Communications

Abstract

Minocycline exerts anti-inflammatory and anti-apoptotic effects distinct from its antimicrobial function. In this study we investigated the effect of this drug on chemotherapy-induced gut damage. Body weight loss results, diarrhea scores, and villi measurements showed that minocycline attenuated the severity of intestinal mucositis induced by 5-fluorouracil (5-FU). Minocycline repressed the expression of TNF-alpha, IL-1beta, and iNOS, decreased the apoptotic index, and inhibited poly(ADP-ribose) polymerase-1 (PARP-1) activity in the mouse small intestine. In vitro experiments showed that minocycline suppressed the upregulation of PARP-1 activity in enterocyte IEC-6 cells treated with 5-FU. In addition, minocycline treatment appeared to enhance the antitumor effects of 5-FU in tumor CT-26 xenograft mice. Our results indicate that minocycline protects mice from gut injury induced by 5-FU and enhances the antitumor effects of 5-FU in xenograft mice. These observations suggest that minocycline treatment may benefit patients undergoing standard cancer chemotherapy by alleviating chemical-associated intestinal mucositis.

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Crossref · 10.1016/j.bbrc.2009.09.041

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Authors
Huang, T.-Y., Chu, H.-C., Lin, Y.-L., Ho, W.-H., Hou, H.-S., Chao, Y.-C., & Liao, C.-L.
Affiliations
  • Tri-Service General Hospital (TW)
  • Institute of Biomedical Sciences, Academia Sinica (TW)
  • National Defense Medical Center (TW)
  • Taipei Tzu Chi Hospital (TW)
Corresponding author
Liao, Ching-Len
Venue
Biochemical and Biophysical Research Communications
Publisher
Elsevier BV
Published
2009-11
Volume
389
Issue
4
Pages
634-639
ISSN
0006-291X (ISSN-L)
OpenAlex
W2032783440
Cited by
59
References
28
Citations by year
2026: 1 · 2025: 2 · 2024: 2 · 2023: 3 · 2022: 3 · 2021: 4 · 2020: 2 · 2019: 5 · 2018: 7 · 2017: 3 · 2016: 4 · 2015: 6 · 2014: 3 · 2013: 8 · 2012: 3
FWCI
1.40
Type
journal article
Language
en
Open access
closed
Locations
  • Biochemical and Biophysical Research Communications (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • National Science Council (NSC98-3112-B-016-003)
  • National Health Research Institutes (NHRI-98A1-0809111)
Topics
  • Life Sciences › Pharmacology, Toxicology and Pharmaceutics › Toxicology › Chemotherapy-induced organ toxicity mitigation
  • Health Sciences › Dentistry › General Dentistry › Oral health in cancer treatment
  • Health Sciences › Medicine › Dermatology › Chemotherapy-related skin toxicity
Keywords
minocycline, 5-fluorouracil, chemotherapy-induced intestinal mucositis, small intestine, PARP1, apoptosis, TNF-α, IL-1β, inducible nitric oxide synthase, IEC-6 cells, anticancer activity
MeSH terms
Poly(ADP-ribose) Polymerase Inhibitors, Poly (ADP-Ribose) Polymerase-1, Animals, Anti-Inflammatory Agents, Non-Steroidal, Antimetabolites, Antineoplastic, Disease Models, Animal, Fluorouracil, Intestine, Small, Male, Mice, Inbred BALB C, Minocycline, Neoplasms, Cytokines, Apoptosis, Xenograft Model Antitumor Assays, Cell Proliferation, Mice, Mucositis, Nitric Oxide Synthase Type II

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