Minocycline attenuates 5-fluorouracil-induced small intestinal mucositis in mouse model
Abstract
Minocycline exerts anti-inflammatory and anti-apoptotic effects distinct from its antimicrobial function. In this study we investigated the effect of this drug on chemotherapy-induced gut damage. Body weight loss results, diarrhea scores, and villi measurements showed that minocycline attenuated the severity of intestinal mucositis induced by 5-fluorouracil (5-FU). Minocycline repressed the expression of TNF-alpha, IL-1beta, and iNOS, decreased the apoptotic index, and inhibited poly(ADP-ribose) polymerase-1 (PARP-1) activity in the mouse small intestine. In vitro experiments showed that minocycline suppressed the upregulation of PARP-1 activity in enterocyte IEC-6 cells treated with 5-FU. In addition, minocycline treatment appeared to enhance the antitumor effects of 5-FU in tumor CT-26 xenograft mice. Our results indicate that minocycline protects mice from gut injury induced by 5-FU and enhances the antitumor effects of 5-FU in xenograft mice. These observations suggest that minocycline treatment may benefit patients undergoing standard cancer chemotherapy by alleviating chemical-associated intestinal mucositis.
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- Authors
- Huang, T.-Y., Chu, H.-C., Lin, Y.-L., Ho, W.-H., Hou, H.-S., Chao, Y.-C., & Liao, C.-L.
- Affiliations
- Tri-Service General Hospital (TW)
- Institute of Biomedical Sciences, Academia Sinica (TW)
- National Defense Medical Center (TW)
- Taipei Tzu Chi Hospital (TW)
- Corresponding author
- Liao, Ching-Len
- Venue
- Biochemical and Biophysical Research Communications
- Publisher
- Elsevier BV
- Published
- 2009-11
- Volume
- 389
- Issue
- 4
- Pages
- 634-639
- ISSN
- 0006-291X (ISSN-L)
- OpenAlex
- W2032783440
- Cited by
- 59
- References
- 28
- Citations by year
- 2026: 1 · 2025: 2 · 2024: 2 · 2023: 3 · 2022: 3 · 2021: 4 · 2020: 2 · 2019: 5 · 2018: 7 · 2017: 3 · 2016: 4 · 2015: 6 · 2014: 3 · 2013: 8 · 2012: 3
- FWCI
- 1.40
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Biochemical and Biophysical Research Communications (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Science Council (NSC98-3112-B-016-003)
- National Health Research Institutes (NHRI-98A1-0809111)
- Topics
- Life Sciences › Pharmacology, Toxicology and Pharmaceutics › Toxicology › Chemotherapy-induced organ toxicity mitigation
- Health Sciences › Dentistry › General Dentistry › Oral health in cancer treatment
- Health Sciences › Medicine › Dermatology › Chemotherapy-related skin toxicity
- Keywords
- minocycline, 5-fluorouracil, chemotherapy-induced intestinal mucositis, small intestine, PARP1, apoptosis, TNF-α, IL-1β, inducible nitric oxide synthase, IEC-6 cells, anticancer activity
- MeSH terms
- Poly(ADP-ribose) Polymerase Inhibitors, Poly (ADP-Ribose) Polymerase-1, Animals, Anti-Inflammatory Agents, Non-Steroidal, Antimetabolites, Antineoplastic, Disease Models, Animal, Fluorouracil, Intestine, Small, Male, Mice, Inbred BALB C, Minocycline, Neoplasms, Cytokines, Apoptosis, Xenograft Model Antitumor Assays, Cell Proliferation, Mice, Mucositis, Nitric Oxide Synthase Type II
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
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