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Cited by 12Fungal and yeast genetics researchLipid metabolism and biosynthesisCancer, Lipids, and Metabolism

A role for MGA2, but not SPT23, in activation of transcription of ERG1 in Saccharomyces cerevisiae

Rice, Caitlin, Cooke, Matthew, Treloar, Natalie, Vollbrecht, Peter, Stukey, Joseph, McDonough, Virginia·2010·Biochemical and Biophysical Research Communications
DOI 10.1016/j.bbrc.2010.11.020
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Abstract

The SaccharomycescerevisiaeMGA2 gene encodes an important regulator of unsaturated fatty acid production, by controlling transcription and mRNA stability of OLE1, the gene encoding the Δ9 fatty acid desaturase. Lipid composition studies indicated that the mga2Δ strain contains elevated relative amounts of squalene when compared to wild-type cells. The deletion of the MGA2 homologue SPT23 did not impact squalene levels. To explore the role of MGA2 in the regulation of sterol synthesis, the transcription of the ERG1 gene, which encodes squalene epoxidase, was studied using an ERG1 promoter-lacZ reporter gene construct. We report here that in addition to MGA2's role in regulation of unsaturated fatty acids, MGA2 is required for full basal expression of ERG1. Mga2p was found to be controlled by a novel regulator in its activation of ERG1, as neither unsaturated fatty acids nor cobalt affected ERG1 expression, as had previously been shown for Mga2p's regulation of OLE1. Further, response to miconazole treatment, which inhibits production of ergosterol at a later step in the sterol biosynthetic pathway and results in up-regulation of several genes in ergosterol synthesis, was not affected in the mga2Δ mutant. In each case, the spt23Δ mutant strain shows similar ERG1 expression to wild-type cells, while the mga2Δ/spt23Δ strain shows reduced ERG1 expression, comparable to the mga2Δ, suggesting that the role of regulation of ERG1 transcription is unique to Mga2p.

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  • Mga2 is required for the full basal expression of ERG1.

    “Finally, Mga2, the transcription factor that controls the expression of the ∆9 fatty acid desaturase Ole1, which is responsible for the synthesis of unsaturated fatty acids, is required for the full basal expression of ERG1 [84].”

    From Regulation of Ergosterol Biosynthesis in Saccharomyces cerevisiae (2020) · cites this paper as [84]

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Authors
Rice, C., Cooke, M., Treloar, N., Vollbrecht, P., Stukey, J., & McDonough, V.
Affiliations
Hope College (US)
Corresponding author
McDonough, Virginia
ORCID
Venue
Biochemical and Biophysical Research Communications
Publisher
Elsevier BV
Published
2010-12
Volume
403
Issue
3-4
Pages
293-297
ISSN
0006-291X (ISSN-L)
DOI
10.1016/j.bbrc.2010.11.020
OpenAlex
W2055624674
Cited by
12
References
28
Citations by year
2026: 1 · 2025: 2 · 2024: 2 · 2020: 3 · 2019: 2 · 2016: 1 · 2013: 1
FWCI
0.13
Type
journal article
Language
en
Open access
closed
Licence
Locations
  • Biochemical and Biophysical Research Communications (publishedVersion)
  • PubMed (publishedVersion)
  • Hope College Digital Commons (Hope College) (submittedVersion)
Funders
Howard Hughes Medical Institute
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Cancer, Lipids, and Metabolism
Keywords
ERG1, squalene epoxidase, transcriptional regulation, sterol biosynthesis, squalene, unsaturated fatty acid biosynthesis, OLE1, ergosterol, Saccharomyces cerevisiae
MeSH terms
Cobalt, Ergosterol, Fatty Acids, Unsaturated, Membrane Proteins, Miconazole, Saccharomyces cerevisiae, Stearoyl-CoA Desaturase, Transcription Factors, Transcriptional Activation, Gene Expression Regulation, Fungal, Gene Expression Regulation, Enzymologic, Gene Deletion, Saccharomyces cerevisiae Proteins, Fatty Acid Desaturases, Squalene Monooxygenase

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