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Kaiso is a key regulator of spleen germinal center formation by repressing Bcl6 expression in splenocytes
Abstract
Kaiso was previously described as a methylated DNA-binding protein and a transcription repressor interacting with the corepressor protein complex NCoR. In the current study, we show that generation-3 Kaiso knockout mice show a phenotype of splenomegaly and large diffused germinal centers (GC). In the spleens of Kaiso knockout mice, Bcl6 (a transcriptional repressor that plays a critical role in GC development in spleen) and c-Myc were highly expressed, while the cell cycle arrest genes p27 (CDKN1B), p21 (CDKN1A) and Gadd45a were downregulated. Chromatin immunoprecipitation (ChIP) and transcription assays suggested that Kaiso represses Bcl6 expression, and in Kaiso knockout mice, derepressed Bcl6 increased cell proliferation by suppressing p27 (CDKN1B), p21 (CDKN1A) and Gadd45a, while upregulating the oncogene c-Myc. Further evidence for Kaiso regulation of splenomegaly was provided by B lymphocyte Ramos cells, in which ectopic KAISO repressed BCL6 and c-MYC expression, while concomitantly increasing the expression of the cell cycle arrestors p21, p27 and Gadd45a. In summary, derepressed Bcl6 expression may be responsible for increases in GC cell proliferation and splenomegaly of Kaiso knockout mice.
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In the citing paper
“KAISO has also been shown to act as a key regulator of spleen germinal centre (GC) formation by repressing Bcl-6 expression in splenocytes [20]”
From Deletion of murine Rhoh leads to de-repression of Bcl-6 via decreased KAISO levels and accelerates a malignancy phenotype in a murine model of lymphoma (2022) · cites this paper as [20]
In the citing paper
“As KAISO knockout mice develop splenomegaly and large diffuse germinal centres associated with increased expression of Bcl-6 and c-Myc”
From Deletion of murine Rhoh leads to de-repression of Bcl-6 via decreased KAISO levels and accelerates a malignancy phenotype in a murine model of lymphoma (2022) · cites this paper as [20]
In the citing paper
“The significant enrichment of KAISO binding at the Bcl-6 promoter by ChIP-qPCR shown here and decreased expression of Bcl-6 in KOTg lymphoma cells after RhoH re-expression strongly suggest that KAISO is a transcriptional repressor of Bcl-6 in this model, which is consistent with previous data published about the role of KAISO in normal GC B cells [20].”
From Deletion of murine Rhoh leads to de-repression of Bcl-6 via decreased KAISO levels and accelerates a malignancy phenotype in a murine model of lymphoma (2022) · cites this paper as [20]
In the citing paper
“Additional studies in human lymphoma cells interrogating RhoH and KAISO expression would be informative, particularly as splenomegaly and increased GC cell proliferation due to increased Bcl-6 and c-Myc expression has been noted in the absence of Kaiso in mice [20].”
From Deletion of murine Rhoh leads to de-repression of Bcl-6 via decreased KAISO levels and accelerates a malignancy phenotype in a murine model of lymphoma (2022) · cites this paper as [20]
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Research works printed but not linked: 19
- Roy, 2005, A role for KAISO–p120ctn complexes in cancer?, Nat. Rev. Cancer, 5, 956, 10.1038/nrc1752 · DOI
- Yoon, 2003, N-CoR mediates DNA methylation-dependent repression through a methyl CpG binding protein KAISO, Mol. Cell, 12, 723, 10.1016/j.molcel.2003.08.008 · DOI · Paper page
- Polo, 2004, Specific peptide interference reveals BCL6 transcriptional and oncogenic mechanisms in B-cell lymphoma cells, Nat. Med., 10, 1329, 10.1038/nm1134 · DOI
- Shaffer, 2000, BCL-6 represses genes that function in lymphocyte differentiation, inflammation, and cell cycle control, Immunity, 13, 199, 10.1016/S1074-7613(00)00020-0 · DOI · Paper page
- Tunyaplin, 2004, Direct repression of prdm1 by Bcl-6 inhibits plasmacytic differentiation, J. Immunol., 173, 1158, 10.4049/jimmunol.173.2.1158 · DOI · Paper page
- Allman, 1996, BCL-6 expression during B-cell activation, Blood, 87, 5257, 10.1182/blood.V87.12.5257.bloodjournal87125257 · DOI
- Baron, 2004, The human BCL6 transgene promotes the development of lymphomas in the mouse, Proc. Natl. Acad. Sci. USA, 101, 14198, 10.1073/pnas.0406138101 · DOI
- Kerckaert, 1993, LAZ3, a novel zinc-finger encoding gene, is disrupted by recurring chromosome 3q27 translocations in human lymphomas, Nat. Genet., 5, 66, 10.1038/ng0993-66 · DOI
- Ye, 1995, Chromosomal translocations cause deregulated BCL6 expression by promoter substitution in B cell lymphoma, EMBO J., 15, 6209, 10.1002/j.1460-2075.1995.tb00311.x · DOI
- Chen, 1998, Heterologous promoters fused to BCL6 by chromosomal translocations affecting band 3q27 cause its deregulated expression during B-cell differentiation, Blood, 91, 603, 10.1182/blood.V91.2.603 · DOI
- Pasqualucci, 1998, BCL-6 mutations in normal germinal center B cells: evidence of somatic hypermutation acting outside Ig loci, Proc. Natl. Acad. Sci. USA, 95, 11816, 10.1073/pnas.95.20.11816 · DOI
- Prokhortchouk, 2006, Kaiso-deficient mice show resistance to intestinal cancer, Mol. Cell. Biol., 26, 199, 10.1128/MCB.26.1.199-208.2006 · DOI
- Yeh, 2003, Abnormal mammary gland development and growth retardation in female mice and MCF7 breast cancer cells lacking androgen receptor, J. Exp. Med., 198, 1899, 10.1084/jem.20031233 · DOI
- Duy, 2011, BCL6 enables Ph+ acute lymphoblastic leukaemia cells to survive BCR-ABL1 kinase inhibition, Nature, 473, 384, 10.1038/nature09883 · DOI
- Duy, 2010, BCL6 is critical for the development of a diverse primary B cell repertoire, J. Exp. Med., 207, 1209, 10.1084/jem.20091299 · DOI
- Paik, 2005, Expression and prognostic implications of cell cycle regulatory molecules, p16, p21, p27, p14 and p53 in germinal centre and non-germinal centre B-like diffuse large B-cell lymphomas, Histopathology, 47, 281, 10.1111/j.1365-2559.2005.02222.x · DOI
- Furlan-Magaril, 2009, Sequential chromatin immunoprecipitation protocol: ChIP-reChIP, Methods Mol. Biol., 543, 253, 10.1007/978-1-60327-015-1_17 · DOI
- Park, 2005, Kaiso/p120-catenin and TCF/beta-catenin complexes coordinately regulate canonical Wnt gene targets, Dev Cell., 8, 843, 10.1016/j.devcel.2005.04.010 · DOI
- Shaffer, 2002, Blimp-1 orchestrates plasma cell differentiation by extinguishing the mature B cell gene expression program, Immunity, 17, 51, 10.1016/S1074-7613(02)00335-7 · DOI
Cited by
Horiguchi, H., Xu, H., Duvert, B., Ciuculescu, F., Yao, Q., Sinha, A., McGuinness, M., Harris, C., Brendel, C., Troeger, A., et al. (2022) · Small GTPases · doi:10.1080/21541248.2021.2019503 · Cited by 3
Metadata
- Authors
- Koh, D.-I., Yoon, J.-H., Kim, M.-K., An, H., Kim, M.-Y., & Hur, M.-W.
- Venue
- Biochemical and Biophysical Research Communications
- Publisher
- Elsevier BV
- Published
- 2013-12
- Volume
- 442
- Issue
- 3-4
- Pages
- 177-182
- ISSN
- 0006-291X
- Cited by
- 9
- References
- 0
- Language
- en
- Funders
- National Research Foundation of Korea
- Ministry of Education, Science and Technology
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- Koh, D.-I., Yoon, J.-H., Kim, M.-K., An, H., Kim, M.-Y., & Hur, M.-W. (2013). Kaiso is a key regulator of spleen germinal center formation by repressing Bcl6 expression in splenocytes. Biochemical and Biophysical Research Communications. https://doi.org/10.1016/j.bbrc.2013.11.046
