Alpha-glucosidase inhibitor 1-Deoxynojirimycin promotes beige remodeling of 3T3-L1 preadipocytes via activating AMPK
Abstract
Obesity is a serious health challenge in the world, and searching effective drugs to cure obesity is of great importance. 1-Deoxynojirimycin (DNJ) is extracted from mulberry leaves and acts as an α-glucosidase inhibitor to lower blood glucose. Recent studies demonstrated that it also has anti-obesity effect, but the mechanisms remain unknown. In our present study, we mainly examined the effects of DNJ on beige remodeling of 3T3-L1 preadipocytes. We observed that DNJ didn't affect the mRNA levels of fatty acid binding protein 4 (aP2), peroxisome proliferator-activated receptor γ (PPARγ), preadipocyte factor-1 (Pref-1) as well as the mitochondrial uncoupling protein 1 (UCP1), PR domain containing protein 16 (PRDM16), transmembrane protein 26 (TMEM26) in undifferentiated preadipocytes. But after inducing 3T3-L1 preadipocytes to differentiation with white or beige adipogenic medium, DNJ significantly reduced aP2, PPARγ and Pref-1 expressions, while up-regulated the expressions of UCP1, PRDM16 and TMEM26, accompanying with decreased lipid deposition. The ratio of p-AMPK/AMPK was up-regulated by DNJ (10 μM) treatment for 10 days, and the effects of DNJ on p-AMPK/AMPK, UCP1 and PRDM16 could be blocked by AMPK inhibitor Compound C. These results demonstrated that hypoglycemic agent DNJ could suppress the adipogenesis during the differentiation of white preadipocytes, and promote the switch of white preadipocytes to beige adipocytes via activating AMPK, which provided new mechanisms for explaining the benefits of DNJ on obesity-related disorders.
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Obesity is influenced by numerous factors, such as energy balance, biological factors, and hormones/cytokines/other factors.
“Evidences showed obesity is influenced by numerous factors, such as the energy balance, biological factors, and hormones/cytokines/other factors [15,16].”
From Separation and Lipid Inhibition Effects of a Novel Decapeptide from Chlorella pyenoidose (2019) · cites this paper as [16]
AMPK is an important mediator in regulating energy balance, and activating AMPK could be a therapy for inhibiting fat accumulation in adipocytes.
“Additionally, adenosine monophosphate-activated protein kinase (AMPK) is an important mediator involved in regulating energy balance in the human body [16], and activating AMPK could be a therapy for inhibiting fat accumulation in adipocytes.”
From Separation and Lipid Inhibition Effects of a Novel Decapeptide from Chlorella pyenoidose (2019) · cites this paper as [16]
Cites
Novel insights of dietary polyphenols and obesity
Wang, S. F., Moustaïd‐Moussa, N., Chen, L., Mo, H., Shastri, A. A., Su, R., Bapat, P., Kwun, I., & Shen, C. (2013) · The Journal of Nutritional Biochemistry · doi:10.1016/j.jnutbio.2013.09.001 · Cited by 903 · see citing works
AMPK: a key regulator of energy balance in the single cell and the whole organism
Hardie, D. G. (2008) · International Journal of Obesity · doi:10.1038/ijo.2008.116 · Cited by 709 · see citing works
Human BAT Possesses Molecular Signatures That Resemble Beige/Brite Cells
Sharp, L. Z., Shinoda, K., Ohno, H., Scheel, D. W., Tomoda, E., Ruiz, L., Hu, H., Wang, L. L., Pavlova, Z., Gilsanz, V., et al. (2012) · PLoS ONE · doi:10.1371/journal.pone.0049452 · Cited by 627 · see citing works
Shan, T., Liang, X., Bi, P., & Kuang, S. (2013) · The FASEB Journal · doi:10.1096/fj.12-225755 · Cited by 301 · see citing works
Wang, S., Liang, X., Yang, Q., Fu, X., Rogers, C. J., Zhu, M. J., Rodgers, B. D., Jiang, Q., Dodson, M. V., & Du, . (2015) · International Journal of Obesity · doi:10.1038/ijo.2015.23 · Cited by 280 · see citing works
PPARγ activation in adipocytes is sufficient for systemic insulin sensitization
Sugii, S., Olson, P., Sears, D. D., Saberi, M., Atkins, A. R., Barish, G. D., Hong, S.-H., Castro, G. L., Yin, Y.-Q., Nelson, M. C., et al. (2009) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0912487106 · Cited by 255 · see citing works
Phytochemistry, pharmacology, and clinical trials of Morus alba
Eric, E., Wei-Chiang, Chan, E. W. C., et al. (2016) · 中国天然药物 · Cited by 242 · see citing works
Pref-1, a Gatekeeper of Adipogenesis
Hudak, C. S. S., & Sul, H. S. (2013) · Frontiers in Endocrinology · doi:10.3389/fendo.2013.00079 · Cited by 169 · see citing works
Deutsch, M. J., Schriever, S. C., Roscher, A. A., et al. (2013) · Analytical Biochemistry · doi:10.1016/j.ab.2013.10.001 · Cited by 145 · see citing works
Ricquier, D. (2016) · Biochimie · doi:10.1016/j.biochi.2016.10.018 · Cited by 135 · see citing works
Pharmacotherapy for the management of obesity
Patel, D. K. (2015) · Metabolism · doi:10.1016/j.metabol.2015.08.001 · Cited by 114 · see citing works
Brite/beige fat and UCP1 — is it thermogenesis?
Keipert, S., & Jastroch, M. (2014) · Biochimica et Biophysica Acta (BBA) - Bioenergetics · doi:10.1016/j.bbabio.2014.02.008 · Cited by 114 · see citing works
Tsuduki, T., Kikuchi, I., Kimura, T., Nakagawa, K., & Miyazawa, T. (2013) · Food Chemistry · doi:10.1016/j.foodchem.2013.02.025 · Cited by 105 · see citing works
Choi, S., Kim, E. S., Jung, J., Marciano, D. P., Jo, A., Koo, J. Y., Choi, S. Y., Yang, Y. R., Jang, H., Kim, E., et al. (2016) · Diabetes · doi:10.2337/db15-1382 · Cited by 100 · see citing works
Carey, A. L., Vorlander, C., Reddy-Luthmoodoo, M., Natoli, A. K., Formosa, M. F., Bertovic, D. A., Anderson, M. J., Duffy, S. J., & Kingwell, B. A. (2014) · PLoS ONE · doi:10.1371/journal.pone.0091997 · Cited by 82 · see citing works
Li, S., Bouzar, C., Cottet‐Rousselle, C., Zagotta, I., Lamarche, F., Wabitsch, M., Tokarska-Schlattner, M., Fischer‐Posovszky, P., Schlattner, U., & Rousseau, D. L. (2016) · Biochimica et Biophysica Acta (BBA) - Bioenergetics · doi:10.1016/j.bbabio.2016.03.009 · Cited by 74 · see citing works
Fat mass- and obesity-associated gene Fto affects the dietary response in mouse white adipose tissue
Ronkainen, J., Huusko, T. J., Soininen, R., Mondini, E., Cinti, F., Mäkelä, K. A., Kovalainen, M., Herzig, K., Järvelin, M., Sebert, S. P., et al. (2015) · Scientific Reports · doi:10.1038/srep09233 · Cited by 67 · see citing works
Yu, W., Gill, T., Wang, L., Du, Y., Ye, H., Qu, X., Guo, J., Cuconati, A., Zhao, K., Block, T. M., et al. (2012) · Journal of Medicinal Chemistry · doi:10.1021/jm300171v · Cited by 59 · see citing works
Ju, H., Chung, J. H., Hwang, J. W., Kim, O. Y., Lee, J.-Y., & Shin, M. (2014) · Food and Chemical Toxicology · doi:10.1016/j.fct.2014.11.001 · Cited by 57 · see citing works
Functions of Prdm16 in thermogenic fat cells
Ishibashi, J., & Seale, P. (2015) · Temperature · doi:10.4161/23328940.2014.974444 · Cited by 56 · see citing works
Lee, S. M. (2013) · Journal of Microbiology and Biotechnology · doi:10.4014/jmb.1209.09043 · Cited by 29 · see citing works
Adipocyte transdifferentiation and its molecular targets
Rajan, S., Gupta, A., Beg, M., Shankar, K., Srivastava, A., Varshney, S., Kumar, D., & Gaikwad, A. N. (2014) · Differentiation · doi:10.1016/j.diff.2014.07.002 · Cited by 29 · see citing works
What Can ‘Brown-ing’ Do For You?
Singh, A. M., & Dalton, S. (2018) · Trends in Endocrinology and Metabolism · doi:10.1016/j.tem.2018.03.002 · Cited by 17 · see citing works
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Separation and Lipid Inhibition Effects of a Novel Decapeptide from Chlorella pyenoidose
Zhang, R., Chen, J., Mao, X., Qi, P., & Zhang, X. (2019) · Molecules · doi:10.3390/molecules24193527 · Cited by 34 · see citing works
Self-citation
- Authors
- Li, A.-N., Chen, J.-J., Li, Q.-Q., Zeng, G.-Y., Chen, Q.-Y., Chen, J.-L., Liao, Z.-M., Jin, P., Wang, K.-S., & Yang, Z.-C.
- Affiliations
- Central South University (CN)
- Corresponding author
- Yang, Zhi-Chun
- Venue
- Biochemical and Biophysical Research Communications
- Publisher
- Elsevier BV
- Published
- 2019-02
- Volume
- 509
- Issue
- 4
- Pages
- 1001-1007
- ISSN
- 0006-291X (ISSN-L)
- OpenAlex
- W2909051369
- Cited by
- 69
- References
- 23
- Citations by year
- 2026: 36 · 2025: 17 · 2024: 5 · 2023: 1 · 2022: 4 · 2021: 3 · 2020: 2 · 2019: 1
- FWCI
- 1.71
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Biochemical and Biophysical Research Communications (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Natural Science Foundation of China (81001464, 81673491)
- National Natural Science Foundation of Hunan Province (2015JJ3140)
- Outstanding Youth Project of the Education Department of Hunan Province (17B036)
- Topics
- Health Sciences › Medicine › Physiology › Adipose Tissue and Metabolism
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Peroxisome Proliferator-Activated Receptors
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Metabolism, Diabetes, and Cancer
- Keywords
- 1-deoxynojirimycin, alpha-glucosidase inhibitors, beige adipocytes, white adipocyte browning, 3T3-L1 preadipocytes, adipogenesis, AMPK signaling, UCP1, PRDM16, obesity, anti-obesity drugs, lipid accumulation, PPARγ
- MeSH terms
- Adipocytes, Beige, Animals, Cell Differentiation, Hypoglycemic Agents, Obesity, Up-Regulation, 1-Deoxynojirimycin, Adipocytes, 3T3-L1 Cells, Adipogenesis, Mice, AMP-Activated Protein Kinases, Glycoside Hydrolase Inhibitors
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