Daily variations in the expression of miR-16 and miR-181a in human leukocytes
Abstract
Circadian rhythms are controlled by a molecular mechanism that is organized in transcriptional and translational feedback loops of gene expression. Recent studies have been demonstrating the involvement of microRNAs (miRs) in post-transcriptional/translational control of circadian rhythms. In the present study we aimed to analyze the daily variations of miR-16 and miR-181a expression in human leukocytes. These miRs were independently associated with hematopoiesis and circadian rhythms in previous studies using experimental models. Peripheral blood from 6 subjects was sampled in a 24 hour period for expression analysis using quantitative real-time PCR (RT-qPCR). Initially, we evaluated the expression stability of RNU6-2, RNU1A-1, RNU5A-1, SNORD-25, SCARNA-17 and SNORA-73A as candidate genes for normalization of RT-qPCR data. The combination of the four most stable genes (SNORA-73A/SCARNA-17/SNORD-25/RNU6-2) was indicated to provide a better normalization of miRs expressions. The results show a daily variation of miR-181a and miR-16 expression in human leukocytes, suggesting a potential participation of these genes in the modulation of the circadian rhythms present in blood cells.
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A 48-hour interval between CSF collections was selected to minimize any effect of an acute immune response after lumbar puncture and to rule out any effect of circadian rhythm.
“Therefore, we selected a time interval of 48 hours to minimize any effect of an acute immune response after lumbar puncture procedures and to rule out any effect of circadian rhythm.”
From Intra- and Inter-individual Variability of microRNA Levels in Human Cerebrospinal Fluid: Critical Implications for Biomarker Discovery (2017) · cites this paper as [30]
A few miRNAs were reported to fluctuate during circadian rhythm, menstrual cycle, or exercise.
“A few miRNAs were reported to fluctuate during circadian rhythm30, menstrual cycle35 or exercise36.”
From Intra- and Inter-individual Variability of microRNA Levels in Human Cerebrospinal Fluid: Critical Implications for Biomarker Discovery (2017) · cites this paper as [30]
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A circadian clock in macrophages controls inflammatory immune responses
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Salemi, M., Mogavero, M. P., Lanza, G., Mongioì, L. M., Calogero, A. E., & Ferri, R. (2022) · Cells · doi:10.3390/cells11121930 · Cited by 39 · see citing works
Yoon, H., Belmonte, K. C., Kasten, T., Bateman, R., & Kim, J. (2017) · Scientific Reports · doi:10.1038/s41598-017-13031-w · Cited by 26 · see citing works
- Authors
- de Siqueira Figueredo, D., Gitaí, D. L. G., & de Andrade, T. G.
- Affiliations
- Universidade Federal de Alagoas (BR)
- Venue
- Blood Cells, Molecules, and Diseases
- Publisher
- Elsevier BV
- Published
- 2015-04
- Volume
- 54
- Issue
- 4
- Pages
- 364-368
- ISSN
- 1079-9796 (ISSN-L)
- OpenAlex
- W2045972166
- Cited by
- 27
- References
- 1
- Citations by year
- 2026: 1 · 2025: 5 · 2024: 1 · 2023: 3 · 2022: 3 · 2019: 3 · 2018: 2 · 2017: 4 · 2016: 4 · 2015: 1
- FWCI
- 2.03
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Blood Cells Molecules and Diseases (publishedVersion)
- PubMed (publishedVersion)
- Funders
- CAPES (PEB-20110329-002-0004-0131)
- CNPq (475597/2009-5)
- Topics
- Life Sciences › Neuroscience › Endocrine and Autonomic Systems › Circadian rhythm and melatonin
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › MicroRNA in disease regulation
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Molecular Biology Research
- Keywords
- miR-16, miR-181a, circadian rhythm, human leukocytes, microRNA expression, diurnal variation, qRT-PCR normalization, reference genes, post-transcriptional regulation, hematopoiesis
- MeSH terms
- Circadian Rhythm, Gene Expression Regulation, Humans, Leukocytes, Male, Reference Standards, Ribonucleoproteins, Genes, Essential, RNA, Small Nucleolar, Gene Expression Profiling, MicroRNAs, Young Adult, Real-Time Polymerase Chain Reaction
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