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Cited by 54Bone Tissue Engineering Materials3D Printing in Biomedical ResearchCellular Mechanics and Interactions

Effect of cyclic mechanical loading on immunoinflammatory microenvironment in biofabricating hydroxyapatite scaffold for bone regeneration

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Zhang, Penghui, Liu, Xizhe, Guo, Peng, Li, Xianlong, He, Zhongyuan, Li, Zhen, Stoddart, Martin J., Grad, Sibylle +5 more·2021·Bioactive Materials
DOI 10.1016/j.bioactmat.2021.02.024PMC PMC7960680
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Abstract

It has been proven that the mechanical microenvironment can impact the differentiation of mesenchymal stem cells (MSCs). However, the effect of mechanical stimuli in biofabricating hydroxyapatite scaffolds on the inflammatory response of MSCs remains unclear. This study aimed to investigate the effect of mechanical loading on the inflammatory response of MSCs seeded on scaffolds. Cyclic mechanical loading was applied to biofabricate the cell-scaffold composite for 15 min/day over 7, 14, or 21 days. At the predetermined time points, culture supernatant was collected for inflammatory mediator detection, and gene expression was analyzed by qRT-PCR. The results showed that the expression of inflammatory mediators ( IL1B and IL8) was downregulated ( p < 0.05) and the expression of ALP ( p < 0.01) and COL1A1 ( p < 0.05) was upregulated under mechanical loading. The cell-scaffold composites biofabricated with or without mechanical loading were freeze-dried to prepare extracellular matrix-based scaffolds (ECM-based scaffolds). Murine macrophages were seeded on the ECM-based scaffolds to evaluate their polarization. The ECM-based scaffolds that were biofabricated with mechanical loading before freeze-drying enhanced the expression of M2 polarization-related biomarkers ( Arginase 1 and Mrc1 , p < 0.05) of macrophages in vitro and increased bone volume/total volume ratio in vivo. Overall, these findings demonstrated that mechanical loading could dually modulate the inflammatory responses and osteogenic differentiation of MSCs. Besides, the ECM-based scaffolds that were biofabricated with mechanical loading before freeze-drying facilitated the M2 polarization of macrophages in vitro and bone regeneration in vivo. Mechanical loading may be a promising biofabrication strategy for bone biomaterials.

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  • It is also crucial to control degradation kinetics, mechanical stimuli, and oxygen concentrations in the transplanted site.

    “It is also crucial to control degradation kinetics, mechanical stimuli, and oxygen concentrations in the transplanted site [210].”

    From Resorbable Biomaterials Used for 3D Scaffolds in Tissue Engineering: A Review (2023) · cites this paper as [210]

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Cited by

Sorted by citations

Authors
Zhang, P., Liu, X., Guo, P., Li, X., He, Z., Li, Z., Stoddart, M. J., Grad, S., Tian, W., Chen, D., Zou, X., Zhou, Z., & Liu, S.
Affiliations
  • Sun Yat-sen University (CN)
  • The First Affiliated Hospital, Sun Yat-sen University (CN)
  • AO Foundation (CH)
  • Beijing Jishuitan Hospital (CN)
Corresponding author
  • Chen, Dafu
  • Zou, Xuenong
  • Zhou, Zhiyu
ORCID
Venue
Bioactive Materials
Publisher
Elsevier BV
Published
2021-10
Volume
6
Issue
10
Pages
3097-3108
ISSN
  • 2097-1192 (ISSN-L)
  • 2452-199X
DOI
10.1016/j.bioactmat.2021.02.024
PMC
PMC7960680
OpenAlex
W3150254463
Cited by
54
References
3
Citations by year
2025: 13 · 2024: 12 · 2023: 12 · 2022: 12 · 2021: 5
FWCI
3.11
Type
journal article
Language
en
Open access
Licence
Locations
  • Bioactive Materials (publishedVersion, cc-by-nc-nd)
  • PubMed Central (publishedVersion, cc-by-nc-nd)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
  • National Natural Science Foundation of China (31900583, 32071341, 32071351, 81772400)
  • Sanming Project of Medicine in Shenzhen (SZSM201911002)
  • Fundamental Research Funds for the Central Universities (19ykzd05)
  • Natural Science Foundation of Guangzhou City (201704030082, 201807010031)
Topics
  • Physical Sciences › Engineering › Biomedical Engineering › Bone Tissue Engineering Materials
  • Physical Sciences › Engineering › Biomedical Engineering › 3D Printing in Biomedical Research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular Mechanics and Interactions
Keywords
cyclic loading, hydroxyapatite scaffolds, bone regeneration, mesenchymal stem cells, osteogenic differentiation, macrophage polarization, M2 macrophages, freeze-drying, IL1B, interleukin-8, Alps, COL1A1, bone biomaterials

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