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Cited by 2443D Printing in Biomedical ResearchHydrogels: synthesis, properties, applicationsCellular Mechanics and Interactions

Biomaterials / bioinks and extrusion bioprinting

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Chen, X.B., Fazel Anvari-Yazdi, A., Duan, X., Zimmerling, A., Gharraei, R., Sharma, N.K., Sweilem, S., Ning, L.·2023·Bioactive Materials
DOI 10.1016/j.bioactmat.2023.06.006PMC PMC10331419
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Abstract

Bioinks are formulations of biomaterials and living cells, sometimes with growth factors or other biomolecules, while extrusion bioprinting is an emerging technique to apply or deposit these bioinks or biomaterial solutions to create three-dimensional (3D) constructs with architectures and mechanical/biological properties that mimic those of native human tissue or organs. Printed constructs have found wide applications in tissue engineering for repairing or treating tissue/organ injuries, as well as in vitro tissue modelling for testing or validating newly developed therapeutics and vaccines prior to their use in humans. Successful printing of constructs and their subsequent applications rely on the properties of the formulated bioinks, including the rheological, mechanical, and biological properties, as well as the printing process. This article critically reviews the latest developments in bioinks and biomaterial solutions for extrusion bioprinting, focusing on bioink synthesis and characterization, as well as the influence of bioink properties on the printing process. Key issues and challenges are also discussed along with recommendations for future research.

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  • Three-dimensional bioprinting builds constructs layer-by-layer using bioinks, typically hydrogels that can include living cells or therapeutic agents, enabling custom-shaped barriers and incorporation of biological components to aid healing.

    “Three-dimensional bioprinting (an additive manufacturing technique) builds constructs layer-by-layer using bioinks—typically hydrogels that can include living cells or therapeutic agents. This technique enables custom-shaped barriers and the incorporation of biological components to aid healing [22].”

    From Biomaterials in Postoperative Adhesion Barriers and Uterine Tissue Engineering (2025) · cites this paper as [22]

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  • Type I collagen has been extensively utilized in anti-adhesion barriers due to its structural stability and capacity to modulate tissue repair processes.

    “Among these, type I collagen has been extensively utilized in anti-adhesion barriers due to its structural stability and capacity to modulate tissue repair processes [22].”

    From Biomaterials in Postoperative Adhesion Barriers and Uterine Tissue Engineering (2025) · cites this paper as [22]

  • Alginate has been widely explored in biomedical applications due to its excellent biocompatibility, non-toxicity, and ability to form hydrogels.

    “Alginate has been widely explored in biomedical applications due to its excellent biocompatibility, non-toxicity, and ability to form hydrogels.”

    From Biomaterials in Postoperative Adhesion Barriers and Uterine Tissue Engineering (2025) · cites this paper as [22]

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Authors
Chen, X. B., Fazel Anvari-Yazdi, A., Duan, X., Zimmerling, A., Gharraei, R., Sharma, N. K., Sweilem, S., & Ning, L.
Affiliations
  • University of Saskatchewan (CA)
  • Cleveland State University (US)
ORCID
Venue
Bioactive Materials
Publisher
Elsevier BV
Published
2023-10
Volume
28
Pages
511-536
ISSN
  • 2097-1192 (ISSN-L)
  • 2452-199X
DOI
10.1016/j.bioactmat.2023.06.006
PMC
PMC10331419
OpenAlex
W4382197409
Cited by
244
References
3
Citations by year
2026: 64 · 2025: 105 · 2024: 72 · 2023: 3
FWCI
5.31
Type
journal article
Language
en
Open access
Licence
Locations
  • Bioactive Materials (publishedVersion, cc-by-nc-nd)
  • PubMed Central (publishedVersion, cc-by-nc-nd)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
  • Saskatchewan Health Research Foundation
  • Canada Foundation for Innovation
  • University of Saskatchewan
  • Natural Sciences and Engineering Research Council of Canada
Topics
  • Physical Sciences › Engineering › Biomedical Engineering › 3D Printing in Biomedical Research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Medicine › Hydrogels: synthesis, properties, applications
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular Mechanics and Interactions
Keywords
bioinks, extrusion-based bioprinting, biomaterials, tissue engineering, 3D tissue constructs, rheology, bioink formulation, growth factors

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