Cited by 87Lipid metabolism and biosynthesisCancer, Lipids, and MetabolismFungal and yeast genetics research

Dynamics of neutral lipid storage and mobilization in yeast

Daum, G., Wagner, A., Czabany, T., Athenstaedt, K.·2007·Biochimie

Abstract

We make use of the yeast Saccharomyces cerevisiae as a flexible experimental system to investigate coordinate pathways of neutral lipid synthesis, storage and mobilization with special emphasis on the role of different organelles in these processes. Recently, a number of new gene products involved in triacylglycerol (TAG) and steryl ester (STE) metabolism were identified in our laboratory and by other groups. STE are synthesized by the two STE synthases Are1p and Are2p, whereas TAG are formed mainly through the action of the two TAG synthases Dga1p and Lro1p with minor contributions of Are1p and Are2p. Once formed, TAG and STE are stored in so-called lipid particles. A dga1Deltalro1Deltaare1Deltaare2Delta quadruple mutant which lacks neutral lipid synthesis and is consequently devoid of lipid particles turned out to be a valuable tool for studying the physiological role of storage lipids and lipid particles. Mobilization of neutral lipid depots occurs through catalysis of TAG lipases and STE hydrolases. Three TAG lipases named Tgl3p, Tgl4p and Tgl5p, and three STE hydrolases named Tgl1p, Yeh1p and Yeh2p were recently identified at the molecular level. Although these hydrolases exhibit overlapping function within the enzyme families, they are specific for TAG and STE, respectively. With the exception of Dga1p, whose activity is partially localized to lipid particles, TAG and STE forming enzymes are restricted to the endoplasmic reticulum. TAG lipases and STE hydrolases are components of lipid particles with the exception of Yeh2p, which is plasma membrane located. Thus, neutral lipid metabolism is not only regulated at the enzyme level but also by the distribution of the components to organelles. The fact that neutral lipid homeostasis is linked to a number of cell biological processes confirms the important role of this class of lipids as cellular modulators or effectors.

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Crossref · 10.1016/j.biochi.2006.06.018

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Authors
Daum, G., Wagner, A., Czabany, T., & Athenstaedt, K.
Affiliations
Graz University of Technology (AT)
Corresponding author
Daum, G.
Venue
Biochimie
Publisher
Elsevier BV
Published
2007-02
Volume
89
Issue
2
Pages
243-248
ISSN
  • 0037-9042 (ISSN-L)
  • 0300-9084
OpenAlex
W2036510236
Cited by
87
References
41
Citations by year
2025: 4 · 2024: 3 · 2023: 2 · 2022: 4 · 2021: 4 · 2020: 2 · 2019: 5 · 2018: 1 · 2017: 3 · 2016: 9 · 2015: 4 · 2014: 3 · 2013: 4 · 2012: 7
FWCI
0.53
Type
journal article
Language
en
Open access
closed
Locations
  • Biochimie (publishedVersion)
  • PubMed (publishedVersion)
Funders
Austrian Science Fund
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Cancer, Lipids, and Metabolism
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
Keywords
neutral lipid storage, lipid mobilization, triglycerides, sterol esters, lipid particles, triacylglycerol lipase, endoplasmic reticulum, organelle targeting, lipid homeostasis, Saccharomyces cerevisiae
MeSH terms
Enzymes, Esters, Hydrolysis, Saccharomyces cerevisiae, Sterols, Triglycerides, Saccharomyces cerevisiae Proteins, Lipid Metabolism

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