Profiling and authentication of human cell lines using short tandem repeat (STR) loci: Report from the National Cell Bank of Iran
Abstract
Assurance of cell line homogeneity and capability of cell contamination detection are among the most essential steps of cell based research. Due to high discriminatory efficiency, low cost and reliability, analysis of short tandem repeats (STR) has been introduced as a method of choice for human cell line authentication. In the present study 13 Combined DNA Index System (CODIS) based STRs along with the gender determination (Amelogenin) gene were utilized to establish a reproducible approach for the authentication of 100 human cell lines deposited in the National Cell Bank of Iran (NCBI), using the polymerase chain reaction (PCR) method. PCR products were subsequently analyzed by polyacrylamide gel electrophoresis (PAGE) and visualized by silver staining followed by gel documentation and software analysis. STR profiles obtained were compared with those of the American Type Culture Collection (ATCC) and the Japanese Collection of Research Bioresource (JCRB) as STR references. We detected 18.8% cross contamination among the NCBI human cell lines. To our knowledge, this is the first report of authentication of human cell lines using the 13 CODIS core STRs combined with Amelogenin.
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Four examples in the non-Chinese model were notably false cell lines (U-251, Hep2, Ej and HBL-100) for which the actual identity had been previously reported, contributing 6.3% (4/64) of the contamination rate.
“including four examples that were notably false cell lines (U-251, Hep2, Ej and HBL-100) for which the actual identity had been previously reported [8–12], contributing 6.3% (4/64) of the contamination rate.”
From Investigation of Cross-Contamination and Misidentification of 278 Widely Used Tumor Cell Lines (2017) · cites this paper as [9]
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- Authors
- Azari, S., Ahmadi, N., Tehrani, M. J., & Shokri, F.
- Affiliations
- Pasteur Institute of Iran (IR)
- Venue
- Biologicals
- Publisher
- Elsevier BV
- Published
- 2007-06
- Volume
- 35
- Issue
- 3
- Pages
- 195-202
- ISSN
- 1045-1056 (ISSN-L)
- OpenAlex
- W2062814183
- Cited by
- 56
- References
- 0
- Citations by year
- 2026: 3 · 2025: 1 · 2021: 2 · 2020: 5 · 2019: 3 · 2018: 3 · 2017: 4 · 2016: 5 · 2015: 2 · 2014: 2 · 2013: 4 · 2012: 4
- FWCI
- 2.32
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Biologicals (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Pasteur Institute of Iran
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Forensic and Genetic Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Molecular Biology Techniques and Applications
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Genetic diversity and population structure
- Keywords
- microsatellite markers, amelogenin, cell line cross-contamination
- MeSH terms
- Base Sequence, Cell Line, Cell Line, Transformed, Female, Humans, Iran, Male, Tissue Banks, Polymerase Chain Reaction, DNA Primers, Microsatellite Repeats, Cell Line, Tumor, Amelogenin
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