The DOI matches its Crossref record; 32 of the 32 references it cites were found in a registry or an index.
Optimization of fibrin scaffolds for differentiation of murine embryonic stem cells into neural lineage cells
Open accessAbstract
The objective of this research was to determine the appropriate cell culture conditions for embryonic stem (ES) cell proliferation and differentiation in fibrin scaffolds by examining cell seeding density, location, and the optimal concentrations of fibrinogen, thrombin, and aprotinin (protease inhibitor). Mouse ES cells were induced to become neural progenitors by adding retinoic acid for 4 days to embryoid body (EB) cultures. For dissociated EBs, the optimal cell seeding density and location was determined to be 250,000 cells/cm(2) seeded on top of fibrin scaffolds. For intact EBs, three-dimensional (3D) cultures with one EB per 400 microL fibrin scaffold resulted in greater cell proliferation and differentiation than two-dimensional (2D) cultures. Optimal concentrations for scaffold polymerization were 10mg/mL of fibrinogen and 2 NIH units/mL of thrombin. The optimal aprotinin concentration was determined to be 50 microg/mL for dissociated EBs (2D) and 5 microg/mL for intact EBs in 3D fibrin scaffolds. Additionally, after 14 days in 3D culture EBs differentiated into neurons and astrocytes as indicated by immunohistochemisty. These conditions provide an optimal fibrin scaffold for evaluating ES cell differentiation and proliferation in culture, and for use as a platform for neural tissue engineering applications, such as the treatment for spinal cord injury.
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Cites
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Self-citationSpinal Cord Repair in Adult Paraplegic Rats: Partial Restoration of Hind Limb Function
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Self-citationControlled release of nerve growth factor enhances sciatic nerve regeneration
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Cited by
Fibrin: A versatile scaffold for tissue engineering applications
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Scaffold: A Novel Carrier for Cell and Drug Delivery
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Advances in 3D cell culture technologies enabling tissue‐like structures to be created in vitro
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Polymer Scaffolds for Biomaterials Applications
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Metadata
- Authors
- Willerth, S. M., Arendas, K. J., Gottlieb, D. I., & Sakiyama-Elbert, S. E.
- Venue
- Biomaterials
- Publisher
- Elsevier BV
- Published
- 2006-12
- Volume
- 27
- Issue
- 36
- Pages
- 5990-6003
- ISSN
- 0142-9612 (ISSN-L)
- OpenAlex
- W2002095196
- Cited by
- 258
- References
- 32
- FWCI
- 6.91
- Type
- journal article
- Language
- en
- Open access
- green
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